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Amine-functionalized, porous silica-coated NaYF4:Yb/Er upconversion nanophosphors for efficient delivery of doxorubicin and curcumin
摘要： Upconversion nanoparticles (UCNP) with unique multi-photon excitation photo-luminescence properties have been extensively explored as novel contrast agents for low-background biomedical imaging. There is an increasing interest in employing UCNPs as carrier for drug delivery as these offers a unique opportunity to combine therapy and diagnostics in one platform (theranostics). In the present work, we report microwave-assisted synthesis of hexagonal NaYF4:Yb/Er UCNPs coated with porous silica and functionalized with amine (UCNP@mSiO2). The UCNP@mSiO2 were investigated for controlled delivery of a chemotherapeutic agent, doxorubicin (DOX, hydrophilic), and a chemosensitizing agent, curcumin (CCM, hydrophobic). The drug loading was relatively higher for DOX (17.4%), in comparison to CCM (8.1%). The cumulative drug release from DOX-loaded UCNP@mSiO2 were 30 and 41% at physiological (7.4) and tumoral (6.4) pH, following a pseudo Fickian release pattern, whereas the release from CCM-loaded UCNP@mSiO2 were 27 and 50% at pH 7.4 and 6.4, following a non-Fickian and pseudo-Fickian release patterns. Both DOX and CCM-loaded UCNP@mSiO2 exhibited pH-dependent controlled drug delivery but the effect was more pronounced for CCM, the hydrophobic chemosensitizer. Cell viability assay using HeLa cells showed that DOX-loaded UCNP@mSiO2 inhibit cell growth in a dose-dependent manner, similar to free DOX, but the cell inhibition activity of free CCM was lower than CCM passively entrapped in UCNP@mSiO2. Confocal microscopy studies revealed cell uptake of both the drug by HeLa cells. Thus, UCNP@mSiO2 exhibited the unique capability to deliver hydrophilic and hydrophobic drugs, individually. UCNP@mSiO2 carrier, equipped with theranostic capabilities, may potentially be used for pH-responsive release of chemotherapeutic agents in cancer environment.
关键词： curcumin,porous silica,doxorubicin,drug delivery,Upconversion nanoparticles
更新于2025-09-23 15:21:01
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One-pot hydrothermal synthesis of nitrogen and sulfur co-doped carbon dots and their application for sensitive detection of curcumin and temperature
摘要： In this study, excitation-independent emission nitrogen and sulfur co-doped fluorescence carbon dots (N,S-CDs) was fabricated by using a simple and efficient one-step hydrothermal treatment with sodium citrate and thiourea as precursor. We found that the obtained N,S-CDs displayed excellent optical properties and emitted strong blue fluorescence under the 365 nm UV lamp. The relative quantum yield was as high as 26.9% using quinine sulfate as reference. The fluorescence of N,S-CDs could be effectively quenched when curcumin(CM) was added into the solution based on the inner filter effect (IFE). The as-prepared N,S-CDs without any modification could be as switch-off fluorescent probe for fluorescence turn-off detection of CM in the range of 0.15–18.0 μmol L?1 with the detection limit of about 0.04 μmol L?1. Moreover, the as-prepared N,S-CDs were successfully employed to the detection of CM in urine samples with satisfactory results. Noticeably, the N,S-CDs showed a distinct temperature-sensitive feature and used to construct fluorescent temperature sensor. Therefore, a convenient approach was proposed that N,S-CDs could be used as a fluorescent probe for rapid and sensitive detection of curcumin and temperature.
关键词： N,S-CDs,Curcumin,Fluorescent sensor,Temperature,Inner filter effect
更新于2025-09-19 17:15:36
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Photoexcitation triggering via semiconductor Graphene Quantum Dots by photochemical doping with Curcumin versus perio-pathogens mixed biofilms
摘要： Background: Recently, antimicrobial photodynamic therapy (aPDT) as an alternative treatment modality has been used adjunctively in the treatment of periodontitis and peri-implantitis. Photosensitizing agents in the form of nanoparticles have been designed for improving the e?ciency of aPTD. Graphene quantum dots are a special type of nanocrystals that can promote aPDT when coupled with curcumin (Cur). The main objective of the present study was to investigate the e?ects of photoexcited GQD-Cur on the metabolic activity of perio-pathogen mixed bio?lms. Materials and methods: GQD-Cur was synthesized and characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS), fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible spectrometry (UV–Vis), and X-ray di?raction (XRD). The cell cytotoxicity e?ect of GQD-Cur was evaluated on primary human gingival ?broblast (HuGu) cells. Perio-pathogen mixed bio?lms including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia photosensitized with GQD doped with Cur were irradiated with a blue LED at a wavelength of 435 ± 20 nm for 1 min, and then bacterial viability measurements were performed. The antimicrobial susceptibility pro?le, bio?lm formation ability, amount of reactive oxygen species (ROS) released, and variations of gene expressions involved in bio?lm formation were assessed. Results: The SEM, DLS, FTIR, UV–Vis spectrometry, and XRD pattern con?rmed that GQD-Cur was synthesized successfully. According to the results, GQD-Cur exhibited no cytotoxicity against HuGu cells. Photoexcited GQD-Cur resulted in a signi?cant reduction in cell viability (93%) and bio?lm formation capacity (76%) of peri-pathogens compared to the control group (P < 0.05). According to the results, a signi?cant concentration-dependent increase in the ROS generation was observed in perio-pathogens mixed cells treated with di?erent doses of GQD-Cur-aPDT. Moreover, rcpA, ?mA, and inpA gene expression pro?les were downregulated by 8.1-, 9.6-, and 11.8-folds, respectively. Conclusions: Based on the results, photoexcited GQD-Cur have a high potency of perio-pathogens suppression in planktonic and bio?lm forms and downregulation of the bio?lm genes expression pattern was exploited as a nanoscale-based platform for periodontitis.
关键词： Curcumin,Antimicrobial photodynamic therapy,Graphene Quantum Dot,Peri-implantitis,Periodontitis
更新于2025-09-19 17:13:59
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Monitoring of active constituents of turmeric ( <i>Curcuma longa</i> L.) rhizome stored under supplemented white LED-light with different light intensities
摘要： Previous research showed possible applications of supplemented light to increase active compounds in harvested underground part of medicinal plants. Accordingly, the effect of supplementing white LED (light emitting diode) at different light intensities on the main constituents (curcumin, demethoxycurcumin, bisdemethoxycurcumin) of turmeric (Curcuma longa L.) rhizomes was investigated every week during storage. Rhizomes were stored for six weeks at room temperature of 24.7-34.5°C and 56-76% RH. During storage, rhizomes were illuminated with varied number (0 (control), 1, 2 or 3 tube (s)) of white LEDs at light intensities of 0 μmol m-2 s-1, 14-15 μmol m-2 s-1, 41-42 μmol m-2 s-1 and 61-62 μmol m-2 s-1, respectively. The LEDs were operated for 6 h (3:00 to 6:00 am and 6:00 to 9:00 pm) daily. Concentrations of curcuminoid, crude extracts and volatile oil fluctuated during storage. There was an increase in these attributes during the first week of storage and then a decline thereafter till week 4. The highest increase in all parameters occurred in week 5 and decreased at week 6 where there were significant differences among treatments (p<.05). Under the treatment using 61-62 μmol m-2 s-1 of white light LEDs, curcuminoid content (4.42% w/w) and crude extract (5.72% w/v) were 10.78 and 16.02% greater than the control. There was no significant difference in volatile oil content (1.10-2.10%).
关键词： crude extract,storage,Curcuma longa,curcumin,Zingiberaceae,dormancy,volatile oil
更新于2025-09-16 10:30:52
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Enhancement of curcumin antitumor efficacy and further photothermal ablation of tumor growth by single-walled carbon nanotubes delivery system <i>in vivo</i>
摘要： Curcumin, a commonly used natural product for antitumor therapy, is unable to achieve full potential due to poor bioavailability. Based on our previous report of a novel delivery system for curcumin using functionalized single-walled carbon nanotubes by phosphatidylcholine and polyvinylpyrrolidone (SWCNT-Cur), we further evaluated SWCNT-Cur’s performance in vivo and characteristics in vitro. SWCNT-Cur significantly increased the blood concentration of curcumin, up to 18-fold, in mice. And in a murine S180 tumor model, SWCNT-Cur exhibited significantly higher inhibition efficacy on tumor growth and no obvious toxicity in main organs. Moreover, photothermal therapy induced by SWCNT under near-infrared radiation further facilitated SWCNT-Cur to inhibit the tumor growth in vivo. In addition, solvent residue is negligible in SWCNT-Cur formulation, and hydrogen bonding was formed between void carriers and curcumin, as demonstrated by GC chromatograph and IR spectra. Furthermore, experiments of confocal microscopy and spectrofluorometer showed that SWCNT-Cur gave a six-fold higher uptake for curcumin compared to native curcumin in human prostate cancer PC-3 cells. In conclusion, curcumin delivery with functionalized SWCNT is a promising strategy to enhance anticancer activity in vivo by enhancing cell uptake and blood concentration, changing physicochemical properties of curcumin and combining phototherapeutic with chemotherapeutic effects.
关键词： cancer therapy,Curcumin,nanocarriers,single-walled carbon nanotubes,photothermal effect
更新于2025-09-16 10:30:52
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Effect of combination of natural dyes and post-TiCl4 treatment in improving the photovoltaic performance of dye-sensitized solar cells
摘要： Herein, dye-sensitized solar cells (DSSCs) have been sensitized with natural red dye (extracted from red spinach) and yellow dye (extracted from turmeric) and five different combinations of red and yellow dyes (at different volume ratios) to determine the optimum combination of red and yellow dyes for the maximum cell efficiency. In addition, the effect of post-TiCl4 treatment in improving cell efficiency has been studied. From UV-vis absorption spectrum, higher absorbance and cumulative absorption properties over the entire visible region show that the combination of red and yellow dyes absorbs light better than the individual red or yellow dyes. DSSCs sensitized with red and yellow dyes have a photoconversion efficiency of 0.416% and 0.921%, respectively. DSSCs sensitized with the combination of red and yellow dyes showed higher cell parameters than the DSSCs sensitized with the individual yellow or red dye. The photoelectrical parameters of DSSCs cosensitized with the optimum combination of dyes (40% red + 60% yellow) were as follows: short-circuit current (Isc) = 5.343 mA, open-circuit voltage (Voc) = 0.493 V, fill factor (FF) = 0.481, and overall photoconversion efficiency = 1.267%. However, after the treatment of FTO (fluorine doped tin oxide) / TiO2 with TiCl4, a significant amount of improvement in the Isc, Voc, and FF was observed. DSSCs fabricated with TiCl4-treated FTO/TiO2 and sensitized with the optimum combination of red and yellow dyes showed the following cell parameters: Isc= 5.829 mA, Voc= 0.498 V, and F$F = 0.541. Hence, the efficiency obtained was 1.572%, which is almost 1.24 times higher than that of the DSSCs fabricated without TiCl4 treatment. Finally, the possible effect of water content in the combination of dyes has been observed with a fixed amount of red dye in the combination.
关键词： Curcumin,Anthocyanin,Combination of dyes,Blocking layer,Natural red dye,Natural yellow dye,DSSC
更新于2025-09-12 10:27:22
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High Amplification of the Antiviral Activity of Curcumin through Transformation into Carbon Quantum Dots
摘要： It is demonstrated that carbon quantum dots derived from curcumin (Cur-CQDs) through one-step dry heating are effective antiviral agents against enterovirus 71 (EV71). The surface properties of Cur-CQDs, as well as their antiviral activity, are highly dependent on the heating temperature during synthesis. The one-step heating of curcumin at 180 °C preserves many of the moieties of polymeric curcumin on the surfaces of the as-synthesized Cur-CQDs, resulting in superior antiviral characteristics. It is proposed that curcumin undergoes a series of structural changes through dehydration, polymerization, and carbonization to form core–shell CQDs whose surfaces remain a pyrolytic curcumin-like polymer, boosting the antiviral activity. The results reveal that curcumin possesses insignificant inhibitory activity against EV71 infection in RD cells [half-maximal effective concentration (EC50) >200 μg mL?1] but exhibits high cytotoxicity toward RD cells (half-maximal cytotoxic concentration (CC50) <13 μg mL?1). The EC50 (0.2 μg mL?1) and CC50 (452.2 μg mL?1) of Cur-CQDs are >1000-fold lower and >34-fold higher, respectively, than those of curcumin, demonstrating their far superior antiviral capabilities and high biocompatibility. In vivo, intraperitoneal administration of Cur-CQDs significantly decreases mortality and provides protection against virus-induced hind-limb paralysis in new-born mice challenged with a lethal dose of EV71.
关键词： carbon quantum dots,curcumin,enterovirus 71,antiviral agents,viral infection
更新于2025-09-11 14:15:04
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Paper sensor of curcumin by fluorescence resonance energy transfer on nitrogen-doped carbon quantum dot
摘要： Paper Sensor detection methods are attractive in wide analytical applications. Presented herein is a paper sensor and ?uorescence methods that was ?rstly developed to detect curcumin (Cur) based on ?uorescence resonance energy transfer (FRET) between nitrogen-doped carbon quantum dots (NCQDs) and Cur. The facile ?uorescent method was demonstrated to detect Cur in the range of 0e2600 mM with a detection limit of 0.13 mM. And facile paper sensor of Cur was fabricated and displayed at concentration of 0 mM, 100 mM, 200 mM, 300 mM, 400 mM, 500 mM and 600 mM, respectively. In additions, it was realized for determination of Cur in real samples including orange juice and curry solution. Compared with the reported methods, the present method is simple, rapid and sensitive for detecting Cur.
关键词： Nitrogen-doped carbon quantum dots (NCQDs),Curcumin (Cur),Fluorescence resonance energy transfer (FRET),Paper-based sensor
更新于2025-09-11 14:15:04
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A curcumin-based NIR fluorescence probe for detection of amyloid-beta (Aβ) plaques in Alzheimer's disease
摘要： Amyloid-beta (Aβ) peptide as one of the main components of senile plaques is closely related to the onset and progression of incurable Alzheimer's disease (AD). In recent years, it is reported that curcumin derivatives have been used as the near-infrared (NIR) fluorescence imaging probes of Aβ plaques for the early diagnosis of AD. To further develop a curcumin-based NIR fluorescent probe for Aβ plaques, in this work, we have synthesized three novel curcumin-based NIR Dyes. Among them, the curcumin derivative Dye 2 showed a significant enhancement in its fluorescence intensity (λem, 635 nm; 19.5-fold increase in quantum yield, Φ = 0.36; Kd, 1.13 μM) after binding to Aβ plaques. Additionally, in vitro and in vivo fluorescence imaging of Aβ plaques strained with Dye 2 confirmed that the compound was a potential probe to detect Aβ plaques in AD. This work opens a perspective to rationally design novel curcumin-based NIR compound for Aβ detection.
关键词： Alzheimer’s Disease,Fluorescence imaging,Amyloid-beta deposits,NIR fluorescence probe,Curcumin,Amyloid-beta plaques
更新于2025-09-09 09:28:46