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Advances in Mid-Infrared Spectroscopy-Based Sensing Techniques for Exhaled Breath Diagnostics
摘要： Human exhaled breath consists of more than 3000 volatile organic compounds, many of which are relevant biomarkers for various diseases. Although gas chromatography has been the gold standard for volatile organic compound (VOC) detection in exhaled breath, recent developments in mid-infrared (MIR) laser spectroscopy have led to the promise of compact point-of-care (POC) optical instruments enabling even single breath diagnostics. In this review, we discuss the evolution of MIR sensing technologies with a special focus on photoacoustic spectroscopy, and its application in exhaled breath biomarker detection. While mid-infrared point-of-care instrumentation promises high sensitivity and inherent molecular selectivity, the lack of standardization of the various techniques has to be overcome for translating these techniques into more widespread real-time clinical use.
关键词： point-of-care (POC),infrared lasers,MIR,photoacoustic spectroscopy,biomarkers,exhaled breath analysis,non-invasive diagnostics,QCL,quantum cascade lasers,mid-infrared
更新于2025-09-23 15:21:01
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Design and preparation of centrifugal microfluidic chip integrated with SERS detection for rapid diagnostics
摘要： A microfluidic SERS chip integrated with blood separation and in-situ detection was designed and fabricated for the rapid detection of clinical blood samples. Each functional unit in the microfluidic SERS chip consist of separation-decantation cavity based on centrifugal separation principle, mixing channels and SERS detection chamber built with integrated nano-Au on Ag film microstructure. The serum creatinine was selected as a typical sample to demonstrate the capability of microfluidic SERS chip. It was found that the creatinine SERS characteristic peaks at 678 cm-1 can be effectively identified and the detection limit could be as low as 4.42 × 10-3 μmol mL-1 in water. The blood samples were also tested in microfluidic SERS chip. The whole separation and test process could be completed within 2 min, which is a significant improvement in the field of creatinine detection. The whole blood of six cases clinical blood samples were also tested, and the results were consistent with the enzymatic results. The developed microfluidic SERS chip has advantages including reduction of the required quantity of blood sample, reusable and easy to operate. It is expected to provide a new method for rapid diagnostics.
关键词： Surface enhanced Raman spectroscopy (SERS),blood separation,centrifugal microfluidic chip,Ag film@nano-Au substrate,rapid diagnostics
更新于2025-09-23 15:21:01
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Diagnostics of polarization purity of x rays by means of Rayleigh scattering
摘要： Synchrotron radiation is commonly known to be completely linearly polarized when observed in the orbital plane of the synchrotron motion. Under actual experimental conditions, however, the degree of polarization of the synchrotron radiation may be lower than the ideal 100%. We demonstrate that even tiny impurities of polarization of the incident radiation can drastically affect the polarization of the elastically scattered light. We propose to use this effect as a precision tool for the diagnostics of the polarization purity of the synchrotron radiation. Two variants of the diagnostics method are proposed. The ?rst one is based on the polarization measurements of the scattered radiation and relies on theoretical calculations of the transition amplitudes. The second one involves simultaneous measurements of the polarization and the cross sections of the scattered radiation and is independent of theoretical amplitudes.
关键词： polarization purity,diagnostics,Rayleigh scattering,x rays,synchrotron radiation
更新于2025-09-23 15:21:01
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Deep transfer learning-based hologram classification for molecular diagnostics
摘要： Lens-free digital in-line holography (LDIH) is a promising microscopic tool that overcomes several drawbacks (e.g., limited field of view) of traditional lens-based microcopy. However, extensive computation is required to reconstruct object images from the complex diffraction patterns produced by LDIH. This limits LDIH utility for point-of-care applications, particularly in resource limited settings. We describe a deep transfer learning (DTL) based approach to process LDIH images in the context of cellular analyses. Specifically, we captured holograms of cells labeled with molecular-specific microbeads and trained neural networks to classify these holograms without reconstruction. Using raw holograms as input, the trained networks were able to classify individual cells according to the number of cell-bound microbeads. The DTL-based approach including a VGG19 pretrained network showed robust performance with experimental data. Combined with the developed DTL approach, LDIH could be realized as a low-cost, portable tool for point-of-care diagnostics.
关键词： LDIH,VGG19,hologram classification,molecular diagnostics,deep transfer learning
更新于2025-09-23 15:21:01
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Fluorescence in situ hybridization (FISH): History, limitations and what to expect from micro-scale FISH?
摘要： In this article, we review an important cytogenetic technique - fluorescence in situ hybridization (FISH) - which is used for obtaining spatial genomic and transcriptomic information. FISH is widely utilized in genomic and cell biological research as well as for diagnostic applications in preventive and reproductive medicine, and oncology. It is the gold standard technique for the detection of chromosomal abnormalities. Despite the high specificity of FISH and the possibility of direct quantitative imaging, some of its key limitations prevent its regular use in diagnostics. To promote the extensive use of FISH for these applications, limitations in assay time and probe consumption will need to be addressed. Microfluidic technologies hold great promise in improving exactly these parameters. In the past two decades, microtechnology has matured and enabled a new line of analysis tools for biomedical and chemical sciences. Incidentally, the convergence of microtechnology with microfluidics is starting to have a decisive impact in the field of medical diagnostics. By miniaturizing implementations of diagnostic assays, the special characteristics of fluid flow in small volumes can be leveraged to modify reaction kinetics and thus reagent delivery time of assays. Here we highlight selected important historical views on FISH, review its current implementations, and provide a perspective on the future developments and the micro-scale implementations of FISH.
关键词： Diagnostics,Hybridization kinetics,Microfluidics,Tissue sections,Fluorescence in situ hybridization
更新于2025-09-23 15:21:01
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Comparison of the Optical Connectivity Method to X-Ray spray measurements in the near field of a diesel injector
摘要： For diesel sprays, the primary breakup processes are only rarely understood due to the high optical density and the resulting difficulties to measure them with extremely high spatial and sufficient temporal resolution. The Optical Connectivity Method (OCM) has been proposed in the last years to allow the determination of the breakup length of the connected liquid core, thus giving a measurement quantity of the primary breakup. In this work, an improved optical setup of the OCM is applied to a three-hole test injector nozzle where several measurement techniques are compared currently under well-defined conditions up to 100 MPa injection pressure. In this work, the direct comparison with X-Ray measurements done at the Advanced Photon Source of the Argonne National Laboratory will be described. This allows an evaluation of the OCM technique and a comparison of the different measurement quantities in the first 500 μm range of the spray. The structure of the spray is measured by X-Ray phase contrast imaging and the fuel mass distribution is measured by X-Ray absorption imaging. A detailed comparison of the two X-Ray techniques and the OCM technique has been possible for the first time. It is found that the measurement data of the spray near field are very congruent with all three methods. Due to this comparison, the measurement of the non-perturbed length, which describes the distance from the nozzle orifice up to the point where the formation of surface disturbances is starting, by the OCM is validated for the first time. Within this non-perturbed length of the spray, the OCM signal is weak before it starts to illuminate from the scattering of the perturbed surface. Thus, the OCM technique can deliver two characteristic length scales, the non-perturbed length and the breakup length , characterizing the primary spray breakup.
关键词： X-Ray diagnostics,Non-perturbed length,Breakup length,Primary breakup,Optical Connectivity Method
更新于2025-09-23 15:21:01
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Laser-generated plasmas in length scales relevant for thin film growth and processing: simulation and experiment
摘要： In pulsed laser deposition, thin film growth is mediated by a laser-generated plasma, whose properties are critical for controlling the film microstructure. The advent of 2D materials has renewed the interest in how this ablation plasma can be used to manipulate the growth and processing of atomically thin systems. For such purpose, a quantitative understanding of the density, charge state, and kinetic energy of plasma constituents is needed at the location where they contribute to materials processes. Here, we study laser-induced plasmas over expansion distances of several centimeters from the ablation target, which is the relevant length scale for materials growth and modification. The study is enabled by a fast implementation of a laser ablation/plasma expansion model using an adaptive Cartesian mesh solver. Simulation outcomes for KrF excimer laser ablation of Cu are compared with Langmuir probe and optical emission spectroscopy measurements. Simulation predictions for the plasma-shielding threshold, the ionization state of species in the plasma, and the kinetic energy of ions, are in good correspondence with experimental data. For laser fluences of 1–4 J cm?2, the plume is dominated by Cu0, with small concentrations of Cu+ and electrons at the expansion front. Higher laser fluences (e.g. 7 J cm?2) lead to a Cu+ -rich plasma, with a fully ionized leading edge where Cu2+ is the dominant species. In both regimes, simulations indicate the presence of a low-density, high-temperature plasma expansion front with a high degree of ionization that may play a significant role in doping, annealing, and kinetically-driven phase transformations in 2D materials.
关键词： pulsed laser deposition,plasma diagnostics,plasma processing of 2D materials,laser plasma simulation,2D materials,laser ablation,plasma assisted processing
更新于2025-09-23 15:19:57
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Spectroscopic diagnostics of continuous and transient microplasma formed in a millimeter wave photonic crystal
摘要： We present spectroscopic measurements of a microplasma formed within a photonic crystal (PhC) at argon gas pressures up to 750 Torr. Continuous and pulsed millimeter waves (MMW) generate plasma at a vacancy defect within the crystal. Rotational gas temperatures of 300-1050 K and electron densities of 0.5-2×1020 m-3 are obtained from the CH rotational emission spectrum and from Stark broadening of the ???? atomic transition. Electron density is found to be weakly dependent on MMW power because the plasma expands within the defect volume at higher power. Pulsed MMWs slightly increase the peak electron density compared to continuous waves with the same peak power. With MMW pulses, a rapid decrease of wave transmission through the PhC is observed at the beginning of plasma formation. During plasma ignition two distinct plasmas are formed, one on the conducting wall of the PhC near the optical access port and a second within the center of the PhC defect. With longer periods between pulses, the weak wall plasma is observed to form before the principal plasma in the defect, which aids in initiating the main plasma.
关键词： photonic crystal,millimeter waves,electron density,spectroscopic diagnostics,microplasma,gas temperature
更新于2025-09-23 15:19:57
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Diode Laser Spectroscopy for Creating Effective Measuring Systems and Their Use in Biological and Medical Research
摘要： In this paper, we present the most important principles of diode laser spectroscopy, which allow one to create fundamentally new measuring systems for diverse applications in biology and medicine. Using the diode laser spectroscopy method and its primary hardware implementation the emission of gas molecules from skin of the hand was registered and ammonia skin emission was found to be dominant. The properties of exhaled air from patients in different states were determined with another special measuring complex of diode laser spectroscopy. It was shown that when the functional state of the body changes, the gas composition of molecular substances that are present in the exhaled air is altered indirectly and their levels vary significantly depending on the health of the individual. Our findings suggest that it is possible to study the specific functional properties of the human body to facilitate assessment of the health of an individual to determine whether pathological processes occur in the body at various stages of their severity using diode laser spectroscopy. This paper presents clinical and experimental data on this issue.
关键词： non-invasive diagnostics,diode lasers,gas biomarkers,diode laser spectroscopy
更新于2025-09-23 15:19:57
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State-of-the-Art Laser Gas Sensing Technologies
摘要： The increasing desire to detect and monitor in different fields such as in environmental air, life sciences, medical diagnostics, and planetary exploration demand the development of innovative sensing systems. Laser spectroscopy-based techniques have the advantages of high sensitivity, non-invasiveness and in situ, real-time observation. Because of these merits, we introduced state-of-the-art laser gas sensing technologies in this Special Issue. A total of 30 papers was received for consideration of publication. Among them, six manuscripts were rejected by the editor in the initial check process without peer review. The remaining manuscripts were all reviewed by at least two reputed reviewers in related fields from the USA, France, Italy, Germany, Russia, and so on. Finally, 16 manuscripts were accepted for publication in Applied Sciences-Basel. We would like to thank all of these numerous reviewers for their effort.
关键词： laser gas sensing,environmental monitoring,spectroscopy,medical diagnostics,planetary exploration
更新于2025-09-23 15:19:57