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Diode Laser Spectroscopy for Creating Effective Measuring Systems and Their Use in Biological and Medical Research

DOI:10.1134/s0006350919060186 期刊:Biophysics 出版年份:2019 更新时间:2025-09-23 15:19:57
摘要: In this paper, we present the most important principles of diode laser spectroscopy, which allow one to create fundamentally new measuring systems for diverse applications in biology and medicine. Using the diode laser spectroscopy method and its primary hardware implementation the emission of gas molecules from skin of the hand was registered and ammonia skin emission was found to be dominant. The properties of exhaled air from patients in different states were determined with another special measuring complex of diode laser spectroscopy. It was shown that when the functional state of the body changes, the gas composition of molecular substances that are present in the exhaled air is altered indirectly and their levels vary significantly depending on the health of the individual. Our findings suggest that it is possible to study the specific functional properties of the human body to facilitate assessment of the health of an individual to determine whether pathological processes occur in the body at various stages of their severity using diode laser spectroscopy. This paper presents clinical and experimental data on this issue.
作者: Ya. Ya. Ponurovskiia,V. Ya. Zaslavskiia,A. I. Nadezhdinskiia,M. V. Spiridonova,D. B. Stavrovskiia,Yu. P. Shapovalova,A. A. Karabinenkob,Yu. M. Petrenkob
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To highlight the most important issues, with an emphasis on the importance of understanding how measurement systems based on the principles of diode laser spectroscopy are created and operate, as well as to analyze new experimental data that were obtained using different hardware implementations of the DLS method.

Measurement of biomarkers in exhaled air by the DLS method characterizes a type of 'metabolic profile' and confirms the natural relationship between the detected biomarkers and metabolic processes that occur in the body, depending on the functional state. The study proves the diagnostic significance of investigating biomarkers in exhaled air in different states of health of people, which does not require special preparation and conditions.

The technical and application constraints of the experiments, as well as potential areas for optimization.

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