Quantum Dots for Monitoring Choline Consumption Process of Living Cells via an Electrostatic Force-Mediated Energy Transfer

摘要： In this work, a ratiometric nanoprobe CdS/ZnS-FB was designed for H2O2 detection based on FRET assay. Furthermore, CdS/ZnS-FB could work for detecting choline (Ch) and acetylcholine (ACh) since H2O2 is the enzyme cascade reaction product. Significantly, choline consumption could also be quantitatively measured by monitoring FRET ratio (I522 /I426). Thus, the biosensor could be applied as a universal tool for the detection of choline consumption of living cells, which provides a good potential for the applications in detecting chemical transmitter and cancer diagnosis.