研究目的
Developing a sensitive sensing strategy to detect choline in serving disease diagnosis and therapy or regulations of life process.
研究成果
The CdS/ZnS-FB probe could realize the dynamically monitoring the living cells’ choline consumption process, which exhibits a promising potential in biomedical research and early clinical analysis or diagnoses of cancer.
1：Experimental Design and Method Selection:
A ratiometric nanoprobe CdS/ZnS-FB was designed for H2O2 detection based on FRET assay.
2：Sample Selection and Data Sources:
The spectra of CdS/ZnS-FB were tested in aqueous solution.
3：List of Experimental Equipment and Materials:
CdS/ZnS quantum dots, boronate-protected fluorescein (FB), choline oxidase (ChOx), acetylcholine esterase (AChE).
4：Experimental Procedures and Operational Workflow:
The optical response of CdS/ZnS-FB to H2O2 was studied, and the application in biosensors was verified through enzyme reaction model that produced H2O
5：Data Analysis Methods:
The change of FRET ratio (I522/I426) was monitored to detect choline consumption.
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CdS/ZnS quantum dots
Used as a donor in FRET assay for H2O2 detection.
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boronate-protected fluorescein (FB)
Used as an acceptor in FRET assay for H2O2 detection.
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choline oxidase (ChOx)
Catalyzes choline to generate betaine aldehyde and H2O2.
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acetylcholine esterase (AChE)
Catalyses hydrolysis of ACh to generate choline.
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