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Phenanthroline-Derivative Functionalized Carbon Dots for Highly Selective and Sensitive Detection of Cu2+ and S2? and Imaging inside Live Cells
摘要： Developing effective methods for the instant detection of Cu2+ and S2? is highly desired in the biological and environmental fields. Herein, a novel fluorescent nanoprobe was elaborately designed and synthesized by grafting a phenanthroline derivative onto the surface of carbon dots (CDs). The obtained functionalized CDs (FCDs) exhibited blue fluorescence (FL) with excellent photostability and possessed a mean diameter around 4 nm. Cu2+ can be selectively captured by the phenanthroline group of FCDs to generate an absorptive complex in situ, leading to obvious quenching of the FCDs’ FL signal through an inner filter effect. Furthermore, the FL of the FCD–Cu2+ can be effectively recovered by S2? anions due to the release of FCDs from the FCD–Cu2+ complex owing to the formation of stable CuS (Ksp = 1.27 × 10?36) between S2? and Cu2+. The detection limits of the FCDs were determined to be 40.1 nM and 88.9 nM for Cu2+ and S2?, respectively. Moreover, this nanoprobe can also be used for the imaging of intracellular Cu2+ and S2?, which shows strong application prospects in the field of biology.
关键词： carbon dots,fluorescent nanoprobe,(2,3-f)-pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid,Cu2+,S2?
更新于2025-09-23 15:23:52
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Single-Cell ATP Content Monitoring during Hyperthermia Cell Death by using Plasmonic Fluorescent Nanoflare
摘要： Gold nanorods-based plasmonic photothermal therapy (AuNRs-PPTT) has been a prospective anti-cancer approach in which AuNRs absorbs near-infrared (NIR) light and converts it into heat, leading to cell death. Investigating molecular energy metabolism of single cells, especially cancer cells, during hyperthermia cell death process is therefore of great significance, as it can help us to better understand the photothermal lethal mechanism of cancer cells and design new photothermal probes more rationally. However, during the AuNRs-PPTT process, how the cells respond to heat stimulation, and how their energy metabolism changes, these basic issues have rarely been studied. Herein, we selected adenosine triphosphate (ATP) as a target molecule, and by preparing a plasmonic and turn-on type fluorescent nanoprobe we examined the ATP metabolism difference between cancerous cells and normal cells during the AuNRs-PPTT process. We found that the fluorescence intensity increased ~ 60% after 5 min laser irradiation as compared to the initial intensity in single HeLa cells, but only ~ 20% increasement was observed for single H8 cells; obviously the increase of ATP content in cancerous cells was notably higher than that in normal cells during the hyperthermia cell death.
关键词： Plasmonic photothermal therapy,Fluorescent nanoprobe,ATP,Gold nanorods,Hyperthermia cell death
更新于2025-09-19 17:13:59
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An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
摘要： Ketamine is a commonly abused drug due to its stimulant, dissociative and hallucinogenic effects. An overdose of ketamine has been found to cause a variety of side effects. Therefore, the identification and quantification of ketamine are of significant importance for clinical purposes and drug seizing. However, conventional methods for ketamine detection possess some disadvantages such as sophisticated procedures, expensive instruments and low sensitivity. Herein, we develop a novel fluorescent nanoprobe for ultrasensitive ketamine detection with signal amplification based on Adenosine Triphosphate (ATP)-fueled target recycling and FRET (fluorescence resonance energy transfer) occurring between the FAM (Fluorescein, tagged with Y-shape DNA) and AuNPs. Based on the combination of FRET and signals circle amplification, the gold nanospheres functionalized with Y-motif DNA (Y@AuNPs) nanoprobe was utilized for effective ketamine detection with the limit of detection (LOD) down to 3 pg mL?1, which was lower than previously reported. Furthermore, the high sensitivity of Y@AuNPs facilitated quantitative analysis in biological media and practical samples.
关键词： Ultrasensitive detection,Ketamine,FRET,ATP-fueled target recycling,Fluorescent nanoprobe
更新于2025-09-16 10:30:52
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Perylene-Based Fluorescent Nanoprobe for Acid-Enhanced Detection of Formaldehyde in Lysosome
摘要： Formaldehyde (FA), as a reactive carbonyl species, is extremely hazardous to human health if its concentration is above normal level. In live cells, lysosome is a main organelle to generate endogenous FA. Thus, the design of facile, stable and sensitive probes for the detection of FA in lysosome is essential. Herein, a self-assembled fluorescent nanoprobe based on homoallylamino substituted perylene (P-FA) has been developed for FA detection in lysosome. P-FA can react with FA along with emission color change from blue to green. P-FA exhibited high sensitivity and selectivity to FA in DMSO solution. In aqueous solution, P-FA self-assembled into uniform sphere-like nanoparticle as a fluorescent nanoprobe. Furthermore, the reaction between the nanoprobe and FA was greatly facilitated at pH 4-5, leading to a lower detection limit (0.96 μM at pH 5) than that in DMSO. In live cells, P-FA nanoprobe achieved long-term tracking of lysosome (over 12 h). The fluorescent nanoprobe was then used for both exogenous and endogenous FA detection. Our work provides a facile and effective strategy for the detection of FA in lysosome.
关键词： Weak Acidity,Perylene,Formaldehyde Detection,Lysosome,Fluorescent Nanoprobe
更新于2025-09-04 15:30:14