- 标题
- 摘要
- 关键词
- 实验方案
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Polydopamine-Based Tumor-Targeted Multifunctional Reagents for Computer Tomography/Fluorescence Dual-Mode Bioimaging-Guided Photothermal Therapy
摘要： Development of multifunctional diagnosis and treatment reagents is very meaningful in clinical application. Herein, we developed a polydopamine-based (PDA-based) tumor targeted multifunctional reagent by surface-initiated atom transfer radical polymerization (ATRP) strategy. First, the targeted PDA nanoparticles were prepared via combining with folic acid (FA) and dopamine. Then ATRP technology was used to graft the europium(III) complexes onto PDA surface (defined as FEDA). A series of detections revealed that the FEDA nanoparticles had been successfully prepared and exhibited a bright X-ray computer tomography (CT) and photoluminescence (PL) dual-mode imaging efficiency and an excellent photothermal therapy (PTT) effect in vivo/in vitro.
关键词： europium(III) complexes,polydopamine,dual-mode imaging-guided photothermal therapy,folic acid targeted,atom transfer radical polymerization
更新于2025-09-23 15:23:52
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Glutathione Triggered Near Infrared Fluorescence Imaging-guided Chemotherapy by Cyanine Conjugated Polypeptide
摘要： Precise detection of tumor environment for cancer diagnosis was strongly demanded for further therapies. Here, a redox-responsive fluorescence switch off/on system PCQ was designed and synthesized conjugated with near infrared (NIR) cyanine dyes (Cy5.5) and relevant quencher (FQ) in mixed polymeric micelles (PCy and PFQ). The mixed PCQ micelles was prepared with two kinds of polymer with poly (oligo (ethylene glycol) methacrylate) (POEGMA) as the hydrophilic shell, in which fluorescence emission was quenched by fluorescence resonance energy transfer (FRET) effect. The FQ was conjugated with POEGMA by disulfide linkage, which could be broke with redox environment such as high glutathione (GSH) concentration in tumor cells. After the PCQ micelles got into tumor cells, PFQ block in PCQ would be disassembled to recompose PCy micelles. During that process, drugs like doxorubicin (DOX) could be loaded inside and formed PCQ@DOX nanoparticles and then released for accurate NIR bioimaging and drug delivery instantly.
关键词： redox responsive,polypeptide,imaging-guided,chemotherapy,Near infrared fluorescence (NIRF)
更新于2025-09-23 15:21:21
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Minimally Invasive MR Imaging‐guided Stereotactic Laser Thermal Ablation in Neurosurgical Cases of Pediatric Lesional Epilepsy: A Didactic Step-by-Step Practical Approach
摘要： MR imaging– guided thermal ablation, an alternative to conventional surgery, is a minimally invasive procedure with a decrease in the hospital length of stay and intensive care unit use. This procedure, with converging imaging, clinical, and videotelemetry data, has had early success in selected pediatric neurosurgical patients with intractable epilepsy. Our purpose was to describe the step-by-step approach used in our institution for minimally invasive MR imaging– guided stereotactic laser thermal ablation, along with some of its benefits. Use of fast single-shot T1- and T2-weighted sequences allows a rapid outline of the catheter position, which is followed by a targeted limited higher-resolution T2-weighted sequence for detailed assessment of the lesion margins and adjacent critical structures. In our experience, diffusion-weighted images and T2-weighted images allow fast assessment of the treatment effect and correlate well with the real-time estimate of cell death and posttreatment area of encephalomalacia. We found that contrast imaging did not change the treatment plan or outcome. Therefore, we adopted a tailored fast thermal ablation treatment imaging protocol that relied on short and fast acquisitions, including DWI and single-shot T1- and T2-weighted sequences, and allowed a decrease in imaging acquisition time to approximately 45 to 60 minutes and allowed the addition of these patients to the regular MR imaging schedule without major disruptions to overall patient flow. This new approach allowed the spectrum of surgical intervention in intractable epilepsy to include MR imaging– guided thermal ablation as a prephase I or II alternative. A detailed procedure description was provided, followed by examples of patients we treated.
关键词： MR imaging–guided thermal ablation,intractable epilepsy,single-shot T1- and T2-weighted sequences,diffusion-weighted images,pediatric neurosurgical patients,minimally invasive
更新于2025-09-11 14:15:04
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Near-infrared nanoparticles based on indocyanine green-conjugated albumin: a versatile platform for imaging-guided synergistic tumor chemo-phototherapy with temperature-responsive drug release
摘要： The aim of this study was to develop a multifunctional theranostic agent based on BSA nanoparticles (NPs), which loaded artemisinin (ART) and co-conjugated with indocyanine green (ICG) and arginine-glycine-aspartic acid (RGD) peptide (RGD-indocyanine green-Bovine Serum Albumin- artemisinin [IBA] NPs). The physicochemical parameters of RGD-IBA NPs were characterized in terms of the particle size, zeta potential, morphology, entrapment efficiency, drug loading, in vitro release behavior, photothermal and photodynamic effect, and in vitro anticancer ability. In vivo fluorescence and thermal imaging as well as antitumor studies were also evaluated. The tumor chemotherapeutic effects of ART and the ability of fluorescence imaging, hyperthermia generation and reactive oxygen species production of ICG and tumor-targeting RGD were integrated to achieve RGD-IBA NPs for imaging-guided tumor-targeted chemotherapy/photothermal/photodynamic therapy (chemo-phototherapy). The RGD-IBA NPs showed enhanced physiological stability and photo-stability compared with free ART and ICG. In addition, they were temperature-responsive; their sizes increased with increasing temperature between 25°C and 55°C, thereby leading to drug release upon the irradiation with near infrared (NIR) laser. In vivo fluorescence images of tumor-bearing mice showed that the RGD-IBA NPs could highly and passively reach the targeted tumor region with maximum accumulation at 24 hours post-intravenous injection. The in vitro and in vivo results demonstrated that the RGD-IBA NPs not only have good biocompatibility, but also are highly efficient tumor synergistic chemo-phototherapeutic agents. Through this study, it was found that RGD-IBA NPs could potentially be a very promising tumor theranostic agent.
关键词： chemo-phototherapy,indocyanine green,imaging-guided tumor therapy,artemisinin,theranostic
更新于2025-09-04 15:30:14