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Gold nanoparticles of different shape for bicolor lateral flow test
摘要： Spherical gold nanoparticles are the most commonly used marker in lateral flow assays. However, the widespread practice of using identical coloration for the test and control zones of test strips can lead to erroneous interpretations of the assay’s results. We propose an immunochromatographic test strip with lines of different colors. For this purpose, gold nanoparticles of different shapes were used, namely blue nanoflowers in the test zone and red gold nanospheres in the control zone. A detailed synthesis procedure for nanoparticles and their conjugates is considered and design parameters for optimal results are described. For the first time, nanoparticles of different shapes have been combined in the test strip with indirect labeling of specific antibodies (via their interaction with labeled secondary antibodies). Using the T-2 toxin (T2T) as an example, an instrumental detection limit of 30 pg/mL and a working range 0.06-0.9 ng/mL were achieved in an analysis of water-organic corn extracts.
关键词： gold nanoparticles,Immunochromatography,test strip,mycotoxin,indirect labeling,gold nanoflowers
更新于2025-09-23 15:23:52
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Comparative Study of Time-Resolved Fluorescent Nanobeads, Quantum Dot Nanobeads and Quantum Dots as Labels in Fluorescence Immunochromatography for Detection of Aflatoxin B1 in Grains
摘要： Label selection is an essential procedure for improving the sensitivity of fluorescence immunochromatography assays (FICAs). Under optimum conditions, time-resolved fluorescent nanobeads (TRFN), quantum dots nanobeads (QB) and quantum dots (QD)-based immunochromatography assays (TRFN-FICA, QB-FICA and QD-FICA) were systematically and comprehensively compared for the quantitative detection of aflatoxin B1 (AFB1) in six grains (corn, soybeans, sorghum, wheat, rice and oat). All three FICAs can be applied as rapid, cost-effective and convenient qualitative tools for onsite screening of AFB1; TRFN-FICA exhibits the best performance with the least immune reagent consumption, shortest immunoassay duration and lowest limit of detection (LOD). The LODs for TRFN-FICA, QB-FICA and QD-FICA are 0.04, 0.30 and 0.80 μg kg?1 in six grains, respectively. Recoveries range from 83.64% to 125.61% at fortified concentrations of LOD, 2LOD and 4LOD, with the coefficient of variation less than 10.0%. Analysis of 60 field grain samples by three FICAs is in accordance with that of LC-MS/MS, and TRFN-FICA obtained the best fit. In conclusion, TRFN-FICA is more suitable for quantitative detection of AFB1 in grains when the above factors are taken into consideration.
关键词： aflatoxin B1,immunochromatography,time-resolved fluorescent nanobeads,quantum dot nanobeads,quantum dot
更新于2025-09-23 15:19:57
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Simultaneous detection of 2, 4-dichlorophenoxyacetic acid and fenitrothion through lanthanide doped β-NaYF4 upconversion nanoparticles with different emitting light colors
摘要： A real-time, on-site rapid and sensitive detection method of low-molecular chemical pollutants was developed by an immunochromatographic strip based on the competitive reaction between the pesticide antigens and polyclonal antibody labeled with upconversion nanoparticles (UCNPs) bio-probes. Two polyclonal antibodies of 2, 4-D and fenitrothion were labeled by β-NaYF4:Yb, Er (green phosphors) and β-NaY(Gd)F4:Yb, Er (red phosphors) UCNPs respectively and dropped to the conjugation pads on the immunochromatographic strip. The competitors of fenitrothion-OVA and 2, 4-D-OVA were immobilized onto a nitrocellulose membrane to form two test lines T1 and T2, respectively. Meanwhile, the goat anti-rabbit IgG was built onto a nitrocellulose membrane to form control lines (C). The detection limits of 2, 4-D and fenitrothion are 5 ng/mL and 12 ng/mL respectively by using the UCNPs-based immunochromatographic strip. The demonstrated results indicated that two di?erent emitting colors UCNPs have great superiority as luminescent bio-probes for simultaneous detection of low-molecular pesticide residues.
关键词： Multi-component detection,2,4-D,Fenitrothion,Immunochromatography,Upconversion nanoparticles
更新于2025-09-09 09:28:46