研究目的
To develop a real-time, on-site rapid and sensitive detection method for low-molecular chemical pollutants using an immunochromatographic strip based on upconversion nanoparticles (UCNPs) for simultaneous detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and fenitrothion.
研究成果
The developed UCNPs-based immunochromatographic strip provides a fast, low-cost, on-site, and high-throughput method for the simultaneous detection of 2,4-D and fenitrothion with detection limits of 5 ng/mL and 12 ng/mL, respectively. This method has potential applications in agricultural and environmental monitoring.
研究不足
The study focuses on the detection of 2,4-D and fenitrothion, and the applicability to other low-molecular-weight pesticide residues needs further exploration. The detection limits, while sensitive, may require optimization for different environmental and food samples.
1：Experimental Design and Method Selection
The study utilized an immunochromatographic strip based on the competitive reaction between pesticide antigens and polyclonal antibodies labeled with UCNPs bio-probes. Two polyclonal antibodies were labeled with different UCNPs for simultaneous detection.
2：Sample Selection and Data Sources
Standard solutions of 2,4-D and fenitrothion in PBS were used to determine the detection limits of the immunochromatographic strip.
3：List of Experimental Equipment and Materials
Included rare earth oxides, oleic acid, 1-octadecene, polyethyleneimine, bovine serum albumin, and various chemicals for synthesis and conjugation. Equipment included XRD-6000 diffractometer, Hitachi S-4800 SEM, and Edinburgh FS5 spectrophotometer.
4：Experimental Procedures and Operational Workflow
Synthesis of UCNPs, conjugation with antibodies, construction of immunochromatographic strips, and detection of 2,4-D and fenitrothion using these strips.
5：Data Analysis Methods
Detection limits were determined based on the concentration of analytes that caused complete invisibility of the test line on the strip.
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