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- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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High-throughput Proteomics Enabled by a Photocleavable Surfactant
摘要： Mass spectrometry (MS)-based proteomics provides unprecedented opportunities for understanding the structure and function of proteins in complex biological systems; however, protein solubility and sample preparation before MS analysis remain a bottleneck preventing high-throughput proteomics. Herein, we report for the first time a high-throughput bottom-up proteomics method enabled by a newly developed MS-compatible photocleavable surfactant, 4-hexylphenylazosulfonate (Azo)[1] that facilitates robust protein extraction, rapid enzymatic digestion (30 min compared to overnight), and subsequent MS-analysis following UV degradation. Moreover, we developed an Azo-aided bottom-up method for efficient analysis of integral membrane proteins, which are key drug targets and are generally underrepresented in global proteomic studies. Furthermore, we demonstrated the unique ability of Azo to serve as an “all-in-one” MS-compatible surfactant for both top-down and bottom-up proteomics, with streamlined workflows for high-throughput proteomics amenable to clinical applications.
关键词： membrane proteomics,high-throughput proteomics,photocleavable surfactant
更新于2025-09-23 15:19:57
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Bactericidal action of ferulic acid with ultraviolet-A light irradiation
摘要： The bactericidal activity of ferulic acid (FA) against various microorganisms was remarkably enhanced by ultraviolet-A (UV-A) irradiation (wavelength, 365 nm). However, the bactericidal mechanism in the photo-combination system has not been evaluated. In the present study, this combined treatment was characterized by investigating associated changes in cellular functions of Escherichia coli, including assessments of respiratory activity, lipid peroxidation, membrane permeability, and damage to DNA and the cell surface. FA adsorbed onto and was incorporated into bacterial membranes, and the affinity resulted in decreased respiratory activity and enhanced lipid peroxidation in the cytoplasmic membrane with low-fluence (1.0 J/cm2) UV-A irradiation. Flow cytometry analysis revealed that additional exposure (8 J/cm2) combined with FA (1 mg/mL) induced increased cell permeability, yielding a 4.8-log decrease in the viable cell count. Morphologically, the treated cells exhibited a bacterial membrane dysfunction, producing many vesicles on the cell surface. However, despite this effect on the cell surface, plasmid DNA transformed into FA-treated E. coli maintained supercoiled integrity with negligible DNA oxidation. Our data strongly suggested that FA functions inside and outside the bacterial membrane; UV-A exposure in the presence of FA then causes increased oxidative modification and subsequent disruption of the bacterial membrane, without causing detectable genotoxicity.
关键词： Ultraviolet-A,Bactericidal action,Ferulic acid,Oxidative stress,Membrane damage
更新于2025-09-23 15:19:57
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[Methods in Molecular Biology] Autoantibodies Volume 1901 (Methods and Protocols) || Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry
摘要： Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.
关键词： Plasma membrane,Autoantibodies,Cell-based assay,Aquaporin 5,Immunofluorescence cytochemistry
更新于2025-09-23 15:19:57
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Proof of Concept for Light Conducting Membrane Substrate for UV-Activated Photocatalysis as an Alternative to Chemical Cleaning
摘要： Adopting an effective strategy to control fouling is a necessary requirement for all membrane processes used in the water/wastewater treatment industry to operate sustainably. The use of ultraviolet (UV) activated photocatalysis has been shown to be effective in mitigating ceramic membrane fouling by natural organic matter. The widely used configuration in which light is directed through the polluted water to the membrane’s active layer suffers from inefficiencies brought about by light absorption by the pollutants and light shielding by the cake layer. To address these limitations, directing light through the substrate, instead of through polluted water, was studied. A UV conducting membrane was prepared by dip coating TiO2 onto a sintered glass substrate. The substrate could successfully conduct UV from a lamp source, unlike a typical alumina substrate. The prepared membrane was applied in the filtration of a humic acid solution as a model compound to study natural organic matter membrane fouling. Directing UV through the substrate showed only a 1 percentage point decline in the effectiveness of the cleaning method over two cleaning events from 72% to 71%, while directing UV over the photocatalytic layer had a 9 percentage point decline from 84% to 75%. Adapting the UV-through-substrate configuration could be more useful in maintaining membrane functionality during humic acid filtration than the current method being used.
关键词： photocatalytic membrane,membrane fouling,Titanium dioxide,water treatment
更新于2025-09-19 17:15:36
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TiO2 coated on PVDF membrane for dye wastewater treatment by a photocatalytic membrane
摘要： This work investigated the membrane modi?cation to increase hydrophilicity aiming for application in a photocatalytic membrane. The modi?ed poly(vinylidene ?uoride) (PVDF) ?at sheet membrane was coated with titanium dioxide (TiO2). The chemical and physical changes of membranes were characterized by contact angle, SEM and porosity. The results found that TiO2 successfully modi?ed the PVDF membrane by decreasing the contact angle from 127.1° to 114.3° on increasing the TiO2 concentration from 0.1 to 1.0 g/L. The contact angles were decreased with the increase of coating time. The water permeation after PVDF membrane modi?cation decreased compared with the original membrane, whereas water ?uxes increased with the increase of TiO2 concentration. TiO2 particles were observed on the membrane surface while the porosity of the membrane coated with TiO2 decreased. The optimum condition of membrane coating was obtained at 1.0 g/L of TiO2 and 60 min of coating time. The decolorization of RB 19 by photolysis and photolysis followed by ?ltration was less than 10%; in contrast, the decolorization of RB 19 by photocatalytic oxidation was as well as a photocatalytic membrane for 60 min. The TiO2 photocatalytic membrane is a feasible technology for RB 19 decolorization in wastewater.
关键词： dye,wastewater treatment,PVDF,membrane modi?cation,photocatalytic membrane
更新于2025-09-19 17:15:36
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Atomic layer deposition for membrane interface engineering
摘要： In many applications, interfaces govern the performance of membranes. Structure, chemistry, electrostatics, and other properties of interfaces can dominate the selectivity, flux, fouling resistance, and other critical aspects of membrane functionality. Control over membrane interfacial properties, therefore, is a powerful means of tailoring performance. In this Minireview, we discuss the application of atomic layer deposition (ALD) and related techniques in the design of novel membrane interfaces. We discuss recent literature in which ALD is used to (1) modify the surface chemistry and interfacial properties of membranes, (2) tailor the pore sizes and separation characteristics of membranes, and (3) enable novel advanced functional membranes.
关键词： membrane interface engineering,pore size tuning,surface modification,functional membranes,atomic layer deposition
更新于2025-09-19 17:15:36
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Axial length estimation error caused by hidden double-peak on partial coherence interferometry in an eye with epiretinal membrane: a case report
摘要： Here we report a patient in whom there was a myopic shift after combined cataract surgery and pars plana vitrectomy against the epiretinal membrane, related to axial measurement estimation error caused by a hidden double-peak appearance on partial coherence interferometry measurement. A 52-year-old female presented with epiretinal membrane and underwent combined cataract surgery and pars plana vitrectomy. Axial length was measured with partial coherence interferometry. Although the signal curve in the summary display showed a single peak, a 1.6 diopter myopic shift occurred. Viewed retrospectively, six of 20 individual signal curves showed a double peak. Most of them showed a higher anterior peak, with only one having a higher posterior peak. The other 14 curves showed a single peak at a similar distance to an anterior peak. The anterior peak appeared to be derived from the epiretinal membrane. The possibility of a double peak should always be considered in patients with epiretinal membrane even if the summary display of the partial coherence interferometry measurement shows a single peak. Checking all signal curves would reduce the risk of missing a hidden double peak.
关键词： master,intraocular lens,epiretinal membrane,double peak
更新于2025-09-19 17:15:36
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Fluorescence resonance energy transfer links membrane ferroportin, hephaestin but not ferroportin, amyloid precursor protein complex with iron efflux
摘要： Iron efflux from mammalian cells is supported by the synergistic actions of the ferrous iron efflux transporter, ferroportin (Fpn) and a multicopper ferroxidase, that is, hephaestin (Heph), ceruloplasmin (Cp) or both. The two proteins stabilize Fpn in the plasma membrane and catalyze extracellular Fe3+ release. The membrane stabilization of Fpn is also stimulated by its interaction with a 22-amino acid synthetic peptide based on a short sequence in the extracellular E2 domain of the amyloid precursor protein (APP). However, whether APP family members interact with Fpn in vivo is unclear. Here, using cyan fluorescent protein (CFP)-tagged Fpn in conjunction with yellow fluorescent protein (YFP)-fusions of Heph and the APP family members APP, APLP1, and APLP2 in HEK293T cells we used fluorescence and surface biotinylation to quantify Fpn membrane occupancy and also measured 59Fe efflux. We demonstrate that Fpn and Heph co-localize, and FRET analysis indicated that the two proteins form an iron-efflux complex. In contrast, none of the full-length, cellular APP proteins exhibited Fpn co-localization or FRET. Moreover, iron supplementation increased surface expression of the iron-efflux complex, and copper depletion knocked down Heph activity and decreased Fpn membrane localization. Whereas cellular APP species had no effects on Fpn and Heph localization, addition of soluble E2 elements derived from APP and APLP2, but not APLP1, increased Fpn membrane occupancy. We conclude that a ferroportin-targeting sequence, K/REWEE, present in APP and APLP2, but not APLP1, helps modulate Fpn-dependent iron efflux in the presence of an active multicopper ferroxidase.
关键词： cell surface protein,hephaestin,iron metabolism,membrane transport,metal transport,Ferroportin,multicopper ferroxidase,APP-like proteins,iron efflux,amyloid precursor protein
更新于2025-09-19 17:15:36
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Flow-Through Self-Standing Porous Silicon Sensor
摘要： A self-standing porous silicon (PS) multilayer structure detached from the substrate by the lift-off method and integrated with a microfluidic cell is presented. Experiments of refractive index changes sensing flow through open-ended pores are reported. We continuously recorded the reflectance spectra of the PS membrane each 30 s and measured the shift as different substances flowed through the structure. The experimental sensitivity values are in good agreement with the theoretical simulations performed.
关键词： membrane,self-standing film,lift-off,sensing,porous silicon,flow-through
更新于2025-09-19 17:15:36
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Aryl triazene derivative immobilized on agarose membrane for selective optical sensing and quantitation of Ni2+ in water
摘要： An aryl triazene (3-hydroxy-3-phenyl triaz-1-en-1-benzoic acid) was synthesized and first utilized as a highly selective ligand for the preparation of a novel optical sensor for nickel ion determination in aqueous solutions. The ligand was chemically bonded to a transparent agarose membrane after its epoxy activation for this purpose. Study of the complex formation between the ligand and some metal ions including Ni2+, Cu2+, UO2 2+, Cr3+, Fe3+, Mg2+, Co2+, Pb2+, Ca2+, and Ag+, in aqueous media, indicated a high selectivity towards Ni2+ and Cu2+ for it. After immobilization of the ligand on the agarose membrane, its selectivity for Ni2+ was substantially increased and a more distinct isosbestic point was observed. In addition, a sharp color change from yellow to green was detected upon contact with a solution of nickel ion at pH 9. The effects of pH, ligand concentration, response time, and interfering ions were carefully studied for the optical sensor. No significant interference from the above metal ions was observed. The absorbance of the membrane at 420 nm was linear for Ni2+ concentrations up to 9 × 10?4 mol L?1 with an R2 of 0.991. A detection limit of 2.74 × 10?6 mol L?1, repeatability of 2.2%, and reproducibility of 4.2% were obtained for the Ni2+ quantitation. The optical sensor was used for the analysis of nickel ion in some river water samples with satisfactory results.
关键词： Agarose membrane,Aryl triazene,Nickel ion,Optical sensor
更新于2025-09-19 17:15:36