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Rapid liquid-phase microextraction of analytes from complex samples on superwetting porous silicon for onsite SALDI-MS analysis
摘要： To simplify the pretreatment process of complex samples is a key step for rapid detection. Herein, we report a single-step method to rapidly extract analytes with liquid-phase microextraction (LPME) from complex samples on a superwetting porous silicon (PSi) for onsite detection with surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The operation time is less than 3 min with this simple method. The limit of detection (LOD) of malachite green in lake water is lowered to 10-13 M, that of verapamil and methadone in whole blood is down to 10-11 M and 10-13 M, in urine is 10-13 M and 10-14 M, respectively; and the ranges of quantification is up to 8 or 9 orders of magnitude with high precision (coefficients of determination (R2) > 0.98) for the complex samples. This method could provide an approach to directly extract target compounds from complex samples on substrate for SALDI-MS analysis.
关键词： SALDI-MS,porous silicon,onsite,rapid liquid-phase microextraction,superhydrophobic
更新于2025-09-23 15:22:29
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Rapid immunological detection of copper ions using fluorescence immunochromatographic assay
摘要： One-step fluorescence immunochromatographic assay (FICA) has been developed using fluorescent microspheres-labelled monoclonal antibody (McAb) probe for the rapid detection of copper ions in herbal plants and soil samples were investigated in this study. The results can be read by the fluorescence reader according to the fitted standard curve equation. Under optimal conditions, detecting copper ions with FICA can be done within 15 min with a LOD (limit of detection) of 0.2 μg/L, and was very useful for the rapid onsite testing. A total of 31 solid samples were pre-treated by microwave digestion combined with cation exchange solid phase extraction and prepared as test samples for FICA and ICP-AES. The two methods showed good relativity with correlation coefficients of 0.924 and 0.969 in herbal plants and soil samples for copper detection, respectively. We hope that our research can help promote the daily monitoring of heavy metal pollution effectively.
关键词： Copper ions,fluorescence immunochromatographic assay,monoclonal antibody,rapid onsite testing
更新于2025-09-09 09:28:46