- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Photomedicine - Advances in Clinical Practice || Nurses and Pharmacists in Interdisciplinary Team of Health Care Providers in Photodynamic Therapy
摘要： Background: The modern treatment is based on wide cooperation between diverse representatives of medical professions. The photodynamic therapy is a noninvasive method of treatment both neoplastic diseases and miscellaneous noncancerous illnesses. It is complementary and competitive in some way to various traditional treatment techniques, including chemotherapy, radiotherapy, and surgery. This review emphasizes the significance of collaboration between specialists engaged in research, development, and practical use of photodynamic therapy. Methods: A literature search of electronic bibliographic databases and scientific publishers was performed. The relevant literature was analyzed to identify articles on the involvement of nurses, pharmacists, physicians, and other representatives in photodynamic therapy treatment. Results: In the photodynamic therapy, the overall success is not only dependent of a single unit. Coordinated actions of representatives possessing expertise in various fields of medical, and natural sciences are necessary both during joint research, development, and during the course of the photodynamic therapy treatment in clinics. Conclusions: The effective interaction between professionals and the division of responsibilities at different stages of therapy can guarantee the successful treatment. During therapy, the most important role belongs to the patient who is responsible for acting in accordance with schedule elaborated by physicians, nurses, and pharmacists.
关键词： hospital pharmacy,nursing,treatment,photodynamic therapy,photosensitizer
更新于2025-09-09 09:28:46
-
Perspective Clinical Study on Effect of 5-Aminolevulinic Acid Photodynamic Therapy (ALA-PDT) in Treating Condylomata Acuminata in Pregnancy
摘要： Objective: To observe the clinical efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in treating vulval condylomata acuminata (CA) in pregnancy. Methods: The clinical efficacies of ALA-PDT on 16 cases of CA in pregnancy as well as cryotherapy on 22 cases of CA in pregnancy were analyzed in this prospective study. Results: The treatment group showed a wart clearance rate of 93.8% after 3 PDT treatments, while the control group showed a wart clearance rate of 72.7% after 3 cryotherapy treatments. After the 3-month follow-up period, the treatment group registered a recurrence rate of 6.3%, whereas the control group recorded a recurrence rate of 36.4%, indicating a statistically significant difference (χ2=4.674, p=0.031<0.05). After the 1-month postpartum follow-up period, the newborns grew and developed well, without any abnormality in physical examinations. Conclusion: ALA-PDT is safe and effective in treating CA in pregnancy.
关键词： cryotherapy,photodynamic therapy (PDT),pregnancy,condylomata acuminata (CA)
更新于2025-09-09 09:28:46
-
Helicenic complexes of lanthanide: influence of f-element on the inter-system crossing efficiency and competition between luminescence and oxygen sensitization.
摘要： The coordination of a helicen functionalized bipyridine to various lanthanide complexes (Ln = Y, Eu, Yb, Gd) led to the formation of an isostructural series. The photophysical study indicated that non-emissive lanthanide act as heavy atoms strongly enhancing the singlet oxygen generation. We also demonstrated that sensitization of the f-f luminescence is in competition with singlet oxygen generation.
关键词： singlet oxygen generation,lanthanide,photodynamic therapy,luminescence,helicen
更新于2025-09-09 09:28:46
-
Comparison of Choroidal Hyperpermeability Change after Photodynamic Therapy and Ranibizumab for Chronic Central Serous Chorioretinopathy
摘要： Purpose: To compare changes in choroidal hyperpermeability after half-energy photodynamic therapy (PDT) and intravitreal ranibizumab in the treatment of chronic central serous chorioretinopathy (CSC). Methods: Post-hoc analysis was performed in a randomized, controlled trial comparing half-energy PDT versus intravitreal ranibizumab for chronic CSC; during the experiments, the other treatment was available for salvage treatment if the original was unsuccessful at 3 months. A commercially available image analysis program (Adobe? Photoshop? CS6 [Adobe Systems, Inc., San Jose, CA]) was used for quantification of change in choriodal hyperpermeability on indocyanine green angiography after half-energy PDT or three consecutive intravitreal injections of ranibizumab. Post-treatment images were subtracted from pre-treatment images after adjustments were made to create images depicting the change in choroidal hyperpermeability with treatment. Integrated gray scale values per area in this image were used for analysis of change in choroidal hyperpermeability. Results: The calculated change in choroidal hyperpermeability was significantly greater in the half-energy PDT group (17.36 ± 8.74) than in the ranibizumab group (6.78 ± 5.03) (p < 0.001). All eyes in the half-energy PDT group showed complete resolution of subretinal fluid, and no significant difference in change of choroidal hyperpermeability was found in eyes that received half-energy PDT as primary or salvage treatment. In the ranibizumab-treated group, subretinal fluid resolution was accomplished in 5 eyes, and these eyes showed a significantly larger decrease in choroidal hyperpermeability when compared with eyes showing poor response (10.31 ± 4.00 vs. 2.74 ± 2.16, p = 0.005). In the successfully treated eyes with ranibizumab, there was no significant difference in choroidal hypopermeability change when compared to half-energy PDT (p = 0.124). Conclusions: Using our novel method of analysis of change in choroidal hyperpermeability following treatment for chronic CSC, greater change was found in eyes with good response, and the superior outcome of half-energy PDT over ranibizumab may be attributed to greater influence on choroidal hyperpermeability.
关键词： Choroidal hyperpermeability,Half-energy photodynamic therapy,Intravitreal ranibizumab injection,Chronic central serous chorioretinopathy
更新于2025-09-09 09:28:46
-
Effects of TONS504?photodynamic therapy on mouse mammary tumor cells
摘要： In the present study, TONS504 (C51H58N8O5I2; molecular weight, 1,116.9), a novel cationic hydrophilic photosensitizer, was synthesized from protoporphyrin IX dimethyl ester through a five-step process according to a patented method for use in photodynamic therapy (PDT). The subcellular localization of TONS504 and the cytotoxic effects of TONS504-mediated PDT in the mouse mammary tumor EMT6 cell line were investigated. TONS504 was localized primarily in the lysosomes and partially in the mitochondria. The cytotoxic effects of TONS504-mediated PDT in the mouse mammary tumor EMT6 cell line were investigated using a WST8 assay and an Oxidative Stress kit. The cell viability values following treatment with 10 μg/ml TONS504 at light energies of 0, 1, 5 and 10 J/cm2 were 92.5, 101.8, 27.7 and 1.8%, respectively. The percentages of reactive oxygen species (ROS)(+) cells following the same treatment were 8.6, 8.5, 29.2 and 70.1%, respectively, whereas the percentages of apoptotic cells were 7.1, 5.6, 24.8 and 48.7%, respectively. The percentages of ROS(+) and apoptotic cells in the group subjected to TONS504-mediated PDT increased in a manner dependent on the TONS504 concentration and light energy. Further studies are required to evaluate the in vivo pharmacokinetics, tissue distribution and photodynamic effects of TONS504.
关键词： photodynamic therapy,apoptosis,mammary tumor,reactive oxygen species,TONS504
更新于2025-09-09 09:28:46
-
Successful Treatment of Hailey-Hailey Disease with Aminolevulinic Acid Photodynamic Therapy
摘要： A 40-year-old man presented with a 3-year history of Hailey-Hailey disease (HHD). Painful axillary and inguinal vesicles, erosions, and plaques existed. There was no family history of HHD. The diagnosis was confirmed by histopathologic examination. He had been treated with topical steroids, vitamin D analogs, and oral antibiotics, and short-term remission had been achieved. As soon as he discontinued the treatment, the lesions relapsed. The patient requested other treatments; thus, aminolevulinic acid photodynamic therapy (ALA-PDT) was offered. After informed consent was obtained, we initiated a course of ALA-PDT, which consisted of five treatment sessions over 2-wk intervals. The patient did not use any topical or systemic therapy during the ALA-PDT treatment. At each visit, 10% ALA cream was applied to the axillae and groin for 3 h, followed by irradiation with red light (623～643 nm). A non-coherent light-emitting diode light source was used with a fluence rate of 20～40 mW/cm2 and a light dose of 37 J/cm2 for 20 min. The patient had mild burning in the lesions during light irradiation, but it was tolerable and did not require local anesthetics or sedatives; the discomfort generally resolved after 2 days. No other side effects were noted. After three sessions of ALA-PDT therapy, the patient was clinically improved with flattening of the keratotic papules and a reduction in erythema and erosions. There was a gradual improvement in the signs and symptoms during the treatment period. A marked decrease in erythema and erosions in the axillary and inguinal regions was achieved after five treatments. The patient had no relapse in the treated areas during a 12-month follow-up period.
关键词： Hailey-Hailey disease,aminolevulinic acid,photodynamic therapy
更新于2025-09-09 09:28:46
-
Susceptibility of <i>In Vitro</i> Melanoma Skin Cancer to Photoactivated Hypericin versus Aluminium(III) Phthalocyanine Chloride Tetrasulphonate
摘要： The sensitivity of human melanoma cells to photoactivated Hypericin (Hyp) compared to aluminium(III) phthalocyanine chloride tetrasulphonate (AlPcS4Cl) is reported in this study. Melanoma cells (A375 cell line) were treated with various concentrations of Hyp or AlPcS4Cl alone, for 1, 4, and 24 hrs; varying doses of laser irradiation alone (594 or 682 nm); or optimal concentrations of PSs combined with laser irradiation. Changes in cell morphology, viability, membrane integrity, and proliferation after treatment of cells were determined using inverted microscopy, Trypan blue cell exclusion, Lactate Dehydrogenase (LDH) membrane integrity, and adenosine triphosphate (ATP) cell proliferation assay, respectively. More than 60% of cell survival was observed when cells were treated with 2.5 ??M of Hyp or AlPcS4Cl alone at all incubation times or with 5 J/cm2 of 594 or 682 nm laser alone. Combination of PSs and respective lasers leads to a statistically significant incubation time-dependent decrease in survival of cells. Flow cytometry using the FITC Annexin V/PI apoptosis kit demonstrated that cell death induced after Hyp-PDT is via early and late apoptosis whereas early apoptosis was the main mechanism observed with AlPcS4Cl-PDT. Hyp-PDT compared to AlPcS4Cl-PDT is indicated to be a more effective cancer cell death inducer in melanoma cells.
关键词： AlPcS4Cl,apoptosis,Hypericin,photodynamic therapy,melanoma
更新于2025-09-09 09:28:46
-
Disinfect <i>Porphyromonas gingivalis</i> biofilm on titanium surface with combined application of chlorhexidine and antimicrobial photodynamic therapy
摘要： Various antimicrobial modalities have been proposed to treat peri-implantitis but resulted in limited outcomes. The aim of this in vitro study was to evaluate the disinfection efficacy of combined application of chlorhexidine digluconate (CHX) and antimicrobial photodynamic therapy (aPDT) of titanium surfaces previously contaminated with a porphyromonas gingivalis biofilm. P. gingivalis biofilms were grown on 32 polished and 32 sandblasted large-grit acid-etched (SLA) titanium surfaces. Titanium disks were allocated into four groups as follows: (i) immersed in phosphate-buffered saline (PBS), (ii) immersed in 0.2% CHX, (iii) application of aPDT, (iv) immersed in 0.2% CHX and subsequent aPDT. Residual bacteria were determined by microbial culture analysis and by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) imaging. Combination protocol (CHX+ aPDT) was the most effective in eradicating P. gingivalis (P < 0.05) on both polished and SLA surfaces. There was no significant difference in the number of remaining P. gingivalis between polished titanium disks and the SLA ones in four groups (P > 0.05). Under the limitation of this study, combined technique of preceding application of CHX and subsequent aPDT was shown to be an efficient method in reducing P. gingivalis numbers in both polished and SLA titanium surfaces.
关键词： chlorhexidine digluconate,Porphyromonas gingivalis,titanium surfaces,peri-implantitis,antimicrobial photodynamic therapy
更新于2025-09-09 09:28:46
-
One-year results of intravitreal ranibizumab combined with reduced-fluence photodynamic therapy for polypoidal choroidal vasculopathy
摘要： Purpose: To compare the 1-year results of intravitreal ranibizumab combined with reduced-fluence photodynamic therapy (RF-PDT) to intravitreal ranibizumab (IVR) alone for eyes with polypoidal choroidal vasculopathy (PCV). Methods: We reviewed the medical records from 47 consecutive patients with PCV (47 na?ve eyes). Seventeen eyes from 17 patients had one IVR treatment combined with RF-PDT followed by two additional IVR treatments (combined group), and 30 eyes from 30 patients were treated with 3 monthly IVR treatments (IVR group). All eyes had a follow-up period of at least 12 months. Results: At 12 months, the mean logarithm of the minimal angle of resolution (logMAR) best-corrected visual acuity (BCVA) significantly improved from 0.55 to 0.38 logMAR units in the combined group (P=0.041) but did not change significantly in the IVR group (P=0.371). The central foveal thickness (CFT) was significantly thinner in both groups at 6 months (P,0.01). Additional IVR injections were required less frequently in the combined group (n=3; 17%) than in the IVR group (n=16; 53%) during the 12 month follow-up (P=0.029). Conclusion: The IVR and RF-PDT combination led to significant BCVA improvements and required fewer additional IVR treatments for at least 12 months in eyes with PCV.
关键词： intravitreal ranibizumab,reduced-fluence photodynamic therapy,polypoidal choroidal vasculopathy
更新于2025-09-09 09:28:46
-
Photomedicine - Advances in Clinical Practice || Photodynamic Therapy
摘要： Photodynamic therapy (PDT) employs light activation of tissue‐localized photosensitizer in an oxygen‐dependent process which initiates oxidative stress, inflammation, and cell death. Photodynamic therapy (PDT) involves the activation of a previously administered photosensitizing agent by visible light to induce tumor necrosis. Photosensitizers are topically applied in the treatment of skin tumors to avoid systemic side effects. The main dermatology indications for topical PDT are superficial nonmelanoma skin cancer and dysplasia, notably superficial basal carcinoma (BCC), Bowen's disease (BD), and actinic keratosis (AK). In this chapter, we evaluated the feasibility and efficacy of aminolevulinic acid (ALA) as a photosensitizer (ALA‐PDT) in combination with CO2 laser in the treat‐ment of dermatological disease from basics to clinic research.
关键词： photosensitizer,photodynamic therapy,Bowen's disease,actinic keratosis,aminolevulinic acid
更新于2025-09-09 09:28:46