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Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters
摘要： In this paper, ?uorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the ?rst time. Based on a ?uorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, ?uorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1–100 μM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 μM) de?ned by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.
关键词： probe,?uorescence,copper nanoclusters,gossypol
更新于2025-11-14 17:04:02
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Benzo[ghi]perylene & Coronene as Ratiometric Reversible Optical Oxygen Nano-Sensor
摘要： The development of novel single molecular probe based optical ratiometric oxygen sensor with simple fabrication, high sensitivity, and linear response is highly desirable. Herein, benzo[ghi]perylene (BzP) and coronene (Cron) were explored as dual-emissive ratiometric oxygen nano-sensor that displayed linear plots at a low probe concentration (6 μM). The sensor fabrication was simple, and the probes exhibited efficient fluorescence quenching of monomer emission by molecular oxygen. However, the excimer emission of probe remained unaffected. Thus a ratiometric method is developed based on the dual emitting luminophore. The method can be used to estimate dissolved oxygen in liquids and 5 – 100 % of oxygen concentration in gaseous mixtures.
关键词： Coronene,Benzo[ghi]perylene,Oxygen sensing,Excimer emission,Ratiometric sensor,Fluorescence probe
更新于2025-11-14 17:04:02
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A fluorescent probe for sequential sensing of MnO <sub/>4</sub><sup>?</sup> and Cr <sub/>2</sub> O <sub/>7</sub><sup>2?</sup> ions in aqueous medium based on a UCNS/TMB nanosystem
摘要： This paper reports the successful design of an UC fluorescence nanosystem for distinguishable and sequential detection of MnO4? and Cr2O72? based on the inner filter effect (IFE) between Gd2O3:Yb,Er upconversion nanospheres (UCNS) and 3,3’,5,5’-tetramethylbenzidine (TMB). The detection of MnO4? was realized by a stoichiometric oxidation reaction between TMB and Mn(VII), which resulted in quenching of the upconversion luminescence (UCL) by the blue product through IFE. The assay of Cr2O72?, in contrast, was based on the formation of complexes and the consumption of the oxidation product of the previous reaction. The sensing of MnO4? and Cr2O72? do not interfere with each other when Pb2+ was added as a masking agent. This provides a new strategy for sensitive and selective detection of MnO4? and Cr2O72?. The detection of MnO4? showed not only a low LOD of 0.243 μM but also high selectivity over other possible interfering ions. The sensitivity assay of MnO4? can be performed in the linear range of 1.85 to 69 μM. An excellent linear relationship was also found in the sensing of Cr2O72?, with a linear range of 18.3 to 250 μM and a LOD of 2.95 μM. The selectivity towards Cr2O72? was also proved to be good. Based on these properties, the UCNS–TMB nanoplatform is a potential chemosensor for both MnO4? and Cr2O72? ions in practical assays for waste water.
关键词： upconversion nanospheres,aqueous medium,UCNS/TMB nanosystem,Cr2O72?,MnO4?,TMB,fluorescent probe,inner filter effect,sequential sensing
更新于2025-11-14 17:03:37
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A near-infrared fluorescence probe for ultrafast and selective detection of peroxynitrite with large Stokes shift in inflamed mouse models
摘要： Peroxynitrite (ONOO?) is a kind of reactive oxygen species (ROS) which is associated with pathogenesis of many diseases. A new near-infrared fluorescence probe (DCM-OH) which based on dicyanomethylene-4H-pyrans to detect endogenous ONOO? was designed and synthesized. The two-photon absorption cross sections and large Stokes shift make the probe deeper issue penetration and lower self-absorption. The obtained results demonstrated that probe DCM-OH could sensitively detect ONOO? with a low detection limit (53 nM). What’s more, probe DCM-OH exhibited an ultrafast response rate (within 5 s) toward ONOO?, which would be in favor of tracking the highly reactive and short-lived ONOO? in the living systems. Moreover, DCM-OH was successfully employed for imaging endogenous ONOO? in HepG2/RAW 264.7 cells and further applied to visualize oxidative stress in mouse model of inflammation.
关键词： In vivo imaging,NIR,Fluorescence probe,Peroxynitrite,Large Stokes shift
更新于2025-11-14 15:29:11
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A mitochondria targetable and viscosity sensitive fluorescent probe and its applications for distinguishing cancerous cells
摘要： The determination of mitochondrial viscosity is of great importance owing to its crucial roles in the diffusion-mediated processes. It has demonstrated that the viscosity in cancer cells is higher than normal cells. Developing of viscosity sensitive fluorescent probes for distinguishing normal cells and cancer cells is necessary. Herein, we present a mitochondrial-targeting fluorescent probe PFV for the detection of viscosity in live cells. It exhibited outstanding sensitivity to viscosity, free from disturbing by ROS/sulfuret even at high concentrations. Equipped with mitochondria targeting ability, PFV was then applied to mitochondrial viscosity detection. Most importantly, by viscosity monitoring PFV successfully differentiated normal hepatic cells and cancerous hepatic cells. The present study would offer a broadly applicable for the determination of viscosity in complex systems.
关键词： fluorescence imaging,mitochondrial viscosity,fluorescent probe,cyanine dye,viscosity
更新于2025-11-14 15:29:11
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Photostable Ratiometric Two-photon Fluorescent Probe for Visualizing Hydrogen Polysulfide in Mitochondria and Its application
摘要： Hydrogen polysulfide (H2Sn) has currently attracted much research interest because it not only plays important physiological function in many biological and health-related events, but also considered as a newfound potent signal transducer. Small-molecule based ratiometric fluorescent probes have advantages in sensitivity and bio-detections but such approaches that intentionally developed for H2Sn detection expected to be mitochondria-accessible are still lacking. In this work, due to that triphenylphosphine group introduced into the molecular scaffold of naphthalimide derivative, Mito-NRT-HP was successfully applied to visualize intracellular H2Sn in mitochondria with excellent aqueous solubility, super photobleaching resistance, favorable cellular membrane permeability and good biocompatibility. This one- and two-photon fluorescent probe with high selectivity and sensitivity (LOD = 0.01 μM) evinced 70-fold enhancement of fluorescence ratio (I546 nm/I478 nm) in the presence of H2Sn over other reactive sulfur species (RSS). The experimental results also give Mito-NRT-HP the potential for mapping the H2Sn distribution in mitochondria and evaluating the H2Sn roles in more biological processes and demonstrated the practical application possibility of Mito-NRT-HP in early diagnosis of LPS-induced acute organ injury.
关键词： mitochondria,fluorescent probe,hydrogen polysulfide,ratiometric,two-photon
更新于2025-11-14 15:29:11
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Tannic acid-derivatized graphitic carbon nitride quantum dots as an “on-off-on” fluorescent nanoprobe for ascorbic acid via copper(II) mediation
摘要： A microwave-assisted hydrothermal route was employed to prepare fluorescent tannic acid (TA)-derivatized graphitic carbon nitride quantum dots. The resulting dots display blue fluorescence (best measured at excitation/emission wavelengths of 350/452 nm) with a quantum yield as high as ~44%. The incorporated TA imparts a fluorescence switching behavior in that very low concentrations of Cu(II) can quench the fluorescence, while (AA) can restore it. It is presumed that AA causes Cu(II) to be transformed to Cu(I). Based on these findings, a fluorometric method was designed for AA detection. The probe allows AA to be detected with a 50 pM limit of detection and a linear analytical range that extends from 0.1 to 200 nM of AA. Real and spiked samples were successfully assayed by the probe to demonstrate its analytical applicability.
关键词： Fluorescence recovery,Metal ions,Graphitic nanosheets,Biomolecules,Polyphenolic compounds,Optical probe,Quantum dots
更新于2025-10-22 19:40:53
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[IEEE 2018 IEEE Conference on Antenna Measurements & Applications (CAMA) - Va?ster?s (2018.9.3-2018.9.6)] 2018 IEEE Conference on Antenna Measurements & Applications (CAMA) - Guidelines for Millimeter-Wave Antenna Measurements
摘要： The characterization of physically small antennas in the millimeter-wave range has proven to be a challenge. Several research groups have developed new antenna measurement systems to be able to characterize millimeter-wave antennas. These measurement systems mainly focus on the establishment of a well-defined connection between test equipment and the antenna-under-test (AUT) for radiation pattern measurements. However, uncertainties affecting the measurement results are not always well understood. We have investigated the effect of misalignment, the effectiveness of an anechoic environment for frequencies up to 90 GHz and the impact of spurious radiation of the probe.
关键词： anechoic environment,probe radiation,60 GHz,probe connection,uncertainty analysis,alignment,Millimeter wave,antenna measurement
更新于2025-09-23 15:23:52
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Diketopyrrolopyrrole Bisphosphonate Conjugate: A New Fluorescent Probe for in Vitro Bone Imaging
摘要： We report the synthesis of a conjugate molecule between an unusual red fluorescent diketopyrrolopyrrole (DPP) unit and a bisphosphonate (BP) precursor using the 'Click Chemistry' strategy to target bone tissue and monitor the interaction. After deep investigation, conjugation through a triazole unit between γ-azido rather than β-azido BP and an alkyne functionalized DPP fluorophore group turned out to be the winning strategy. Visualization of the DPP-BP conjugate on osteoclasts as well as the specific anti-resorption activity was successfully demonstrated.
关键词： fluorescent probe,in vitro imaging,osteoporosis,bisphosphonate,diketopyrrolopyrrole
更新于2025-09-23 15:23:52
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Subchronic intravenous toxicity study of biofunctional ZnO and its application as a fluorescence probe for cell-specific targeting
摘要： Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early cancer is a great challenge. Herein, we choose the visible‐light emitting zinc oxide non–core/shell type nanoparticle (NP) fluorophores (ZHIE) as prototypical materials. We have reported on these materials previously. The results showed that the ZHIE NPs exhibited good water solubility and good biocompatibility. This study was conducted to investigate the toxicity of ZHIE NPs when intravenously administered to mice repeatedly at the dose required for successful tumor imaging in vivo. Anti‐macrophage‐1 antigen (Mac1), a macrophage differentiation antigen, antibody‐conjugated ZHIE NPs successfully realized targeted imaging of murine macrophage cell line Raw264.7 cells. In conclusion, ZHIE NPs are not toxic in vivo and antibody‐conjugated ZHIE NPs have great potential in applications, such as single cell labeling.
关键词： subchronic toxicity,zinc oxide (ZnO) nanoparticles,fluorescence probe
更新于2025-09-23 15:23:52