研究目的
To investigate the toxicity of ZHIE NPs when intravenously administered to mice repeatedly at the dose required for successful tumor imaging in vivo and to demonstrate their application as a fluorescence probe for cell-specific targeting.
研究成果
ZHIE NPs are not toxic in vivo at the studied doses and can be effectively used as fluorescence probes for targeted imaging of specific cells, such as macrophages, showing potential for applications in single-cell labeling and cancer diagnosis.
研究不足
The study was conducted only on mice, and the results may not directly translate to humans. The tail vein injury from repeated injections might have affected serum biochemical measurements. The specificity and efficacy in vivo were not fully explored, and further studies are needed for clinical translation.
1：Experimental Design and Method Selection:
The study involved preparing ZHIE NPs and conjugating them with anti-Mac1 antibody for targeted imaging. Toxicity was assessed through subchronic intravenous administration in mice, with methods including LD50 determination, serum biochemical analysis, histopathological assessment, and zinc level measurement.
2：Sample Selection and Data Sources:
Female ICR mice (8-12 weeks old) were used, obtained from CLEA Japan. Raw
3：7 cells and other cell lines were used for in vitro imaging. List of Experimental Equipment and Materials:
2 Equipment included a Vortex mixer, centrifuge, fluorescence microscope (Nikon Eclipse), automatic serum analyzer (Hitachi model 7060), microplate reader (Bio-Rad model 680), electronic weighing balance (Mettler), and materials such as zinc acetate dihydrate, ethyl isocyanatoacetate, ethylene diamine, anti-Mac1 antibody, EDC, N-hydroxysulfosuccinimide, PBS, BSA, paraformaldehyde, isoflurane, EDTA, trichloroacetic acid, and hexametaphosphoric acid.
4：Experimental Procedures and Operational Workflow:
ZHIE NPs were synthesized and conjugated with anti-Mac1 antibody. For toxicity study, mice were administered ZHIE NPs intravenously biweekly for 12 weeks. Blood and tissue samples were collected for analysis. Fluorescence imaging was performed on fixed cells.
5：Data Analysis Methods:
Serum biochemical parameters were analyzed using an automatic analyzer. Zinc levels were measured with a colorimetric assay using a microplate reader. Histopathological slides were assessed microscopically. Statistical analysis included mean ± SD and significance testing.
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