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Effect of anions on fluorescence quenching of Rhodamine B–Pluronic F127 complex
摘要： Pluronic F127 has been widely used for controlled release of drugs. The aggregation behaviour and solubilisation properties of pluronics can be affected by addition of salts. This work reports the studies on the changes in micellar assemblies of F127 due to addition of inorganic anions (KCl, KBr, KI and KNO3) in Pluronic F127–Rhodamine B system. This was monitored by using UV–vis spectroscopy, fluorescence spectroscopy and fluorescence lifetime spectra. The change in fluorescence spectrum due to the presence of quenchers was attributed to the change in microenvironment of the micelle. The quenching depends on the ability of the fluorophore to transfer energy to the quencher. Out of all four salts, KI was observed to be the best quencher for the above system.
关键词： Rhodamine B (Rh B),quenchers,spectroscopy studies,Pluronic F127,micelle
更新于2025-09-23 15:23:52
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A strategy for preparing non-fluorescent graphene oxide quantum dots as fluorescence quenchers in quantitative real-time PCR
摘要： In recent years, graphene oxide quantum dots (GOQDs) have emerged as novel nanomaterials for optical sensing, bioimaging, clinical testing, and environmental testing. However, GOQDs demonstrate unique photoluminescence properties, with GOQDs having quantum limitations and edge effects that often affect the accuracy of the test results in the sensory field. Herein, GOQDs with a large content of hydroxyl groups and low fluorescence intensity were first prepared via an improved Fenton reaction in this study, which introduces a large amount of epoxy groups to break the C–C bonds. The synthesized GOQDs show no significant variation in the fluorescence intensity upon ultraviolet and visible light excitations. We further utilized the GOQDs as fluorescence quenchers for different fluorescent dyes in real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and verified that the addition of GOQDs (5.3 mg ml?1) into a qRT-PCR system could reduce the background fluorescence intensity of the reaction by fluorescence resonance energy transfer (FRET) during its initial stage and its non-specific amplification, and improve its specificity. In addition, the qRT-PCR method could detect two different lengths of DNA sequences with a high specificity in the 104 to 1010 copies per ml range. It is of paramount importance to carry out further investigations to establish an efficient, sensitive, and specific RT-PCR method based on the use of GOQD nanomaterials as fluorescence quenchers.
关键词： Fenton reaction,fluorescence quenchers,DNA detection,quantitative real-time PCR,graphene oxide quantum dots
更新于2025-09-23 15:19:57