1：Experimental Design and Method Selection:

The study used UV–vis spectroscopy, fluorescence spectroscopy, and fluorescence lifetime measurements to monitor changes in micellar assemblies of Pluronic F127 due to anion addition. Stern–Volmer and Lineweaver–Burk equations were employed to analyze quenching mechanisms.

2：Sample Selection and Data Sources:

Samples were prepared using Rhodamine B dye, Pluronic F127 surfactant, and inorganic salts (KCl, KBr, KI, KNO3) as quenchers. Triply distilled water was used as the solvent.

3：List of Experimental Equipment and Materials:

Instruments included Shimadzu 1700 UV–visible spectrophotometer, Fluoromax-4 fluorescence spectrophotometer, and Horiba Jobin Yvon TCSPC lifetime instrument. Materials were Pluronic F127, Rhodamine B, KCl, KBr, KI, KNO3, and methanol.

4：Experimental Procedures and Operational Workflow:

Rhodamine B was dissolved in methanol, evaporated, and diluted with water to specific concentrations. Pluronic F127 was added to achieve 5% w/v concentration. Quencher solutions (0.1 M) were prepared and added to the dye-surfactant mixture in varying concentrations (0.01 to 0.1 M). Spectra were recorded immediately after mixing to prevent photoreactions.

5：1 M) were prepared and added to the dye-surfactant mixture in varying concentrations (01 to 1 M). Spectra were recorded immediately after mixing to prevent photoreactions. Data Analysis Methods:

5. Data Analysis Methods: Data were analyzed using Stern–Volmer plots for quenching constants and Lineweaver–Burk plots for association and dissociation constants. Fluorescence lifetime data were used to calculate bimolecular quenching constants.