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Graphene quantum dot based charge-reversal nanomaterial for nucleus-targeted drug delivery and efficiency controllable photodynamic therapy
摘要： Graphene quantum dots (GQD), the new zero-dimensional carbon nanomaterial, has been demonstrated as a promising material for biomedical applications due to its good biocompatibility and low toxicity. However, the integration of multiple therapeutic approaches into a nano-sized platform based on the GQD has not been explored yet to our best knowledge. In this report, we regulate the generation of reactive oxygen species (ROS) when using the GQD as a photosensitizer by varying the doping amount of nitrogen atoms to achieve efficiency controllable photodynamic therapy (PDT). On the other hand, charge-reversal (3-Aminopropyl) triethoxysilane (APTES) was employed to conjugate on the surface of GQD for nucleus targeting drug delivery for the first time. The treatment outcome of produced ROS and nucleus-targeting drug delivery was investigated by fluorescence imaging. The results demonstrated that the N-GQD-DOX-APTES in dual roles as a drug carrier and photosensitizer could achieve nucleus-targeting delivery and strong ROS production simultaneously. This approach provides a promising strategy for the development of multifunctional therapy in one nano platform for biomedical applications.
关键词： nucleus-targeted drug delivery,Graphene quantum dots,nitrogen doped graphene quantum dots,charge-reversal,photodynamic therapy
更新于2025-11-21 11:24:58
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In Situ Synthesis of Fluorescent Mesoporous Silica–Carbon Dot Nanohybrids Featuring Folate Receptor-Overexpressing Cancer Cell Targeting and Drug Delivery
摘要： Multifunctional nanocarrier-based theranostics is supposed to overcome some key problems in cancer treatment. In this work, a novel method for the preparation of a fluorescent mesoporous silica–carbon dot nanohybrid was developed. Carbon dots (CDs), from folic acid as the raw material, were prepared in situ and anchored on the surface of amino-modified mesoporous silica nanoparticles (MSNs–NH2) via a microwave-assisted solvothermal reaction. The as-prepared nanohybrid (designated MSNs–CDs) not only exhibited strong and stable yellow emission but also preserved the unique features of MSNs (e.g., mesoporous structure, large specific surface area, and good biocompatibility), demonstrating a potential capability for fluorescence imaging-guided drug delivery. More interestingly, the MSNs–CDs nanohybrid was able to selectively target folate receptor-overexpressing cancer cells (e.g., HeLa), indicating that folic acid still retained its function even after undergoing the solvothermal reaction. Benefited by these excellent properties, the fluorescent MSNs–CDs nanohybrid can be employed as a fluorescence-guided nanocarrier for the targeted delivery of anticancer drugs (e.g., doxorubicin), thereby enhancing chemotherapeutic efficacy and reducing side effects. Our studies may provide a facile strategy for the fabrication of multifunctional MSN-based theranostic platforms, which is beneficial in the diagnosis and therapy of cancers in future.
关键词： Targeted drug delivery,Fluorescence imaging,Mesoporous silica nanoparticles,Carbon dots,Chemotherapy
更新于2025-11-14 14:48:53
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Polypyrrole microcapsules loaded with gold nanoparticles: Perspectives for biomedical imaging
摘要： We report the facile preparation of polypyrrole microcapsules with a hydrophobic liquid core that is loaded with gold nanoparticles. Through the oxidative polymerization of pyrrole, the polymer is deposited onto the surface of the droplets, which results in the encapsulation of both the liquid phase and the metal nanoparticles. We demonstrate the preparation of the microcapsules loaded with organic solvents (toluene, hexane) or 2-oxoheptyl isothiocyanate (new promising anticancer agent) as the liquid cores and stable or radioactive gold nanoparticles (Au-197 or Au-198 isotopes). The resulting microcapsules have been demonstrated as promising agents for medical applications such as computed tomography or gamma imaging. Moreover, the capsules can be applied as drug carriers, which has been shown in vitro on cancer and normal cell cultures.
关键词： Targeted drug delivery,Gold nanoparticles doped with Au-198,Computed tomography,Anticancer agents,Polypyrrole microcapsules,Gamma imaging
更新于2025-09-23 15:22:29
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Bypassing pro-survival and resistance mechanisms of autophagy in EGFR-positive lung cancer cells by targeted delivery of 5FU using theranostic Ag <sub/>2</sub> S quantum dots
摘要： Targeted drug delivery systems that combine imaging and therapeutic functions in a single structure have become very popular in nanomedicine. Near-infrared (NIR) emitting Ag2S quantum dots (QDs) are excellent candidates for this task. Here, we have developed PEGylated Ag2S QDs functionalized with Cetuximab (Cet) antibody and loaded with an anticancer drug, 5-fluorouracil (5FU). These theranostic QDs were used for targeted NIR imaging and treatment of lung cancer using low (H1299) and high (A549) Epidermal Growth Factor Receptor (EGFR) overexpressing cell lines. The Cet conjugated QDs effectively and selectively delivered 5FU to A549 cells and provided significantly enhanced cell death associated with apoptosis. Interestingly, while treatment of cells with free 5FU activated autophagy, a cellular mechanism conferring resistance to cell death, these EGFR targeting multimodal QDs significantly overcame drug resistance compared to 5FU treatment alone. The improved therapeutic outcome of 5FU delivered to A549 cells by Cet conjugated Ag2S QDs is suggested as the synergistic outcome of enhanced receptor mediated uptake of nanoparticles, and hence the drug, coupled with suppressed autophagy even in the absence of addition of an autophagy suppressor.
关键词： lung cancer,EGFR,theranostic,Ag2S quantum dots,autophagy,targeted drug delivery,5-fluorouracil
更新于2025-09-16 10:30:52
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Designing luminescent ruthenium prodrug for precise cancer therapy and rapid clinical diagnosis
摘要： The effective design of a targeted drug delivery system could improve the therapeutic efficacy of anticancer drugs by reducing their undesirable adsorption and toxic side effects. Here, an RGD-peptide functionalized and bioresponsive ruthenium prodrug (Ru-RGD) was designed for both cancer therapy and clinical diagnosis. This prodrug can be selectively delivered to cervical tumor sites to enhance theranostic efficacy. The benzimidazole-based ligand of the complex is susceptible to acidic conditions so, after reaching the tumor microenvironment, ligand substitution occurs and the therapeutic drug is released. The deep-red emissions produced by both one-photon and two-photon excitation increases the potential of Ru-RGD for use in the deep tissue imaging of 3D tumor spheroids. The specific accumulation of the Ru prodrug in tumor sites allows for precise tumor diagnosis and therapy in vivo. Luminescence staining of 38 clinical patient specimens shows that Ru-RGD exhibits differences in binding capability between cervical cancer and normal tissue, with a sensitivity of 95% and a specificity of 100%. This study thus provides an approach for the effective design and application of targeted metal complexes in cancer therapy and clinical diagnosis.
关键词： targeted drug delivery,cancer theranosis,rapid clinical diagnosis,two-photon imaging,tumor microenvironment
更新于2025-09-10 09:29:36