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- 摘要
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- 实验方案
- 产品
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Simultaneous fluorometric determination of the DNAs of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus by using an ultrathin metal-organic framework (type Cu-TCPP)
摘要： Ultrathin (<10 nm) nanosheets of a metal-organic framework (MOF-NSs) were prepared in high-yield and scalable production by a surfactant-assisted one-step method. The MOF-NSs possess distinguished affinity for ssDNA but not for dsDNA. This causes the fluorescence of the labeled DNA to be quenched. On binding to the target DNA (shown here for Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus), the labeled duplex is released and the fluorescence of the label is restored. The labels Texas Red, Cy3 and FAM were used and give red, red or green fluorescence depending on the kind of pathogen. The detection limits are 28 pM, 35 pM and 15 pM for the gene segments of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus, respectively.
关键词： FRET,Surfactant-assisted synthesis,Two dimensional nanomaterials,Pathogens,Fluorescence sensor,Multiplex detection
更新于2025-09-23 15:22:29
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Ultrathin Tellurium Oxide/Ammonium Tungsten Bronze Nanoribbon for Multi-Modality Imaging and Second Near-Infrared Region Photothermal Therapy
摘要： Developing nano-photothermal agents (PTAs) with satisfied photothermal conversion efficiency (PTCE) in the second NIR window (1000-1350 nm, NIR II) holds great promise for enhanced photothermal therapy (PTT) effect. Herein, we develop a NIR-II PTA with advanced PTCE, based on a new two-dimensional (2D) ultrathin tellurium oxide/ammonium tungsten bronze (TeO2/(NH4)xWO3) nanoribbons (TONW NRs). The doped ammonia ions mediated-free electrons injection into the LUMO band of WO3 combined with the electronic transitions between W6+ ions and the lone pair of electrons in Te atoms achieve excellent NIR absorption of TONW NRs resulted from localized surface plasmon resonance (LSPR). The polyethylene glycol functionalized TONW NRs (PEG-TONW NRs) exhibit good stability and biocompatibility, displaying a PTCE high to 43.6%, surpassing many previous nano-PTAs active in the NIR II region, leading to remarkable tumor ablation ability both in vitro and in vivo. Meanwhile, advanced X-ray computed tomography (CT) and photoacoustic (PA) imaging capability of PEG-TONW NRs were also realized. Given the admirable photothermal effect in NIR II region, good biocompatibility and advanced CT/PA imaging diagnosis capability, the novel PEG-TONW NRs is promising in future personalized medicine application.
关键词： photothermal therapy,multimodal imaging,tellurium oxide,two-dimensional nanomaterials,ammonium tungsten bronze,the second NIR window
更新于2025-09-23 15:22:29