研究目的
Developing nano-photothermal agents with high photothermal conversion efficiency in the second near-infrared window for enhanced photothermal therapy and multimodal imaging.
研究成果
The PEG-TONW NRs exhibit high photothermal conversion efficiency (43.6%) in the NIR-II window, excellent biocompatibility, and effective multimodal imaging capabilities (CT and PA), leading to significant tumor ablation in vitro and in vivo, making them promising for personalized medicine applications.
研究不足
The study involves preliminary in vivo toxicity assessments; further systematic studies are needed to fully understand excretion and toxicology. The tissue penetration depth in NIR-II is limited to 6-8 mm, and the synthesis may require optimization for scalability.
1：Experimental Design and Method Selection:
The study synthesized TeO2/(NH4)xWO3 nanoribbons (TONW NRs) using a liquid-liquid interface-mediated method with Te powder and paratungstate as precursors, inspired by localized surface plasmon resonance mechanisms. PEGylation was performed using DSPE-PEG for stability and biocompatibility.
2：Sample Selection and Data Sources:
MCF-7 and A549 cancer cell lines were used for in vitro studies, and tumor-bearing mice (Balb/c) for in vivo experiments.
3：List of Experimental Equipment and Materials:
Equipment includes SEM, TEM, HRTEM, AFM, XRD, XPS, FT-IR, DLS, UV-Vis spectrophotometer, thermal imager, CT scanner, PA imaging system, ICP-OES, flow cytometer, confocal microscope. Materials include Te powder, (NH4)10[H2W12O42]·4H2O, DSPE-PEG, cell culture media, and various chemicals for synthesis and assays.
4：Experimental Procedures and Operational Workflow:
Synthesis of TONW NRs, PEGylation, characterization (morphology, composition, optical properties), photothermal efficiency measurement, cytotoxicity assays, in vitro and in vivo imaging (CT and PA), biodistribution studies, and in vivo PTT efficacy evaluation.
5：Data Analysis Methods:
Statistical analysis of cell viability, calculation of PTCE using heat transfer equations, image analysis for CT and PA signals, and ICP-OES for element quantification.
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SEM
Characterization of morphology for TONW NRs
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TEM
Transmission electron microscopy for imaging and analysis
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HRTEM
High-resolution TEM for lattice fringe analysis
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AFM
Atomic force microscopy for thickness measurement
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XRD
X-ray diffraction for phase composition analysis
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XPS
X-ray photoelectron spectroscopy for elemental composition
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FT-IR
Fourier-transform infrared spectroscopy for verifying PEGylation
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DLS
Dynamic light scattering for size distribution analysis
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UV-Vis spectrophotometer
UV-Vis absorption measurement
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Thermal imager
Infrared thermal imaging for temperature monitoring
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CT scanner
X-ray computed tomography for imaging
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PA imaging system
Photoacoustic imaging for contrast enhancement
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ICP-OES
Inductively coupled plasma mass spectrometry for element quantification
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Flow cytometer
Flow cytometry for cell death analysis
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Confocal microscope
Confocal imaging for cell staining analysis
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NIR laser
1064 nm
Laser irradiation for photothermal therapy
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DSPE-PEG
DSPE-PEG-2000
Polyethylene glycol functionalization for stability and biocompatibility
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