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A Novel Perylene Derivative/Luminol Nanocomposite as Strong Electrochemiluminescence Emitter for Construction of Ultrasensitive MicroRNA Biosensor
摘要： In this work, an electrochemiluminescent (ECL) biosensor was constructed on the basis of amino-modified 3,4,9,10-perylenete-tracarboxylic dianhydride/luminol (PTC-NH2/Lu) nanocomposite as emitter and bipedal DNA walker signal amplification strategy for ultrasensitive detection of microRNA-21 (miRNA-21). The PTC-NH2/Lu nanocomposite was prepared as signal tag via π-π stacking molecular assembly, in which amino-modified 3,4,9,10-perylenete-tracarboxylic dianhydride (PTC-NH2) as a novel co-reaction accelerator significantly enhanced the ECL emission of luminol-H2O2 system. Moreover, target miRNA-21 triggered bipedal DNA walker was powered by toehold-mediated strand displacement reaction (TSDR) for signal amplification. Consequently, the proposed ECL biosensor achieved ultrasensitive detection of miRNA-21 with linear range from 100 aM to 100 pM and limit of detection 33 aM. Simultaneously, the biosensor was also successfully applied to detect target miRNA-21 in lysates from human cancer cells. As a result, this work constructed a new signal amplification platform, exhibiting great application potential in biomedical analysis and early clinical diagnostics.
关键词： PTC-NH2/Lu nanocomposite,electrochemiluminescence,co-reaction accelerator,π-π stacking molecular assembly,bipedal DNA walker
更新于2025-09-23 15:19:57
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An ultrasensitive aptasensor based on self-enhanced Au nanoclusters as highly efficient electrochemiluminescence indicator and multi-site landing DNA walker as signal amplification
摘要： Gold nanoclusters (Au NCs) have been shown to be prospective nanoscale electrochemiluminescence (ECL) materials that are being extensively explored in bioanalysis. However, the low ECL efficiency of Au NCs has been a bottleneck barrier for their better bioapplications. To overcome this disadvantage, a low oxidation potential co-reactant N,N-diisopropylethylenediamine (DPEA) was first used to prepare self-enhanced Au NCs (Au-DPEA NCs) for drastically enhancing the ECL efficiency of Au NCs in this study. In addition, an efficient multi-site landing DNA walker with multidirectional motion track and rapid payloads release compared to directional DNA walker was constructed for converting target mucin 1 (MUC1) to intermediate DNA and achieving significant signal amplification. On the basis of the Au-DPEA NCs as efficient ECL signal labels and multi-site landing DNA walker as signal amplification strategy, an ECL aptasensor was established for the ultrasensitive detection of MUC1 in the range from 1 fg mL-1 to 1 ng mL-1 with a limit of detection down to 0.54 fg mL-1. The results demonstrated that the present study opened a new research direction for the development of high-efficiency Au NCs indicator as well as ultrasensitive ECL sensing platform for applications in clinical and bioanalysis.
关键词： Electrochemiluminescence,N-diisopropylethylenediamine,self-enhanced Au nanoclusters,N,multi-site landing DNA walker
更新于2025-09-19 17:15:36
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A Novel Fluorescence Switch for MicroRNA Imaging in Living Cells based on DNAzyme Amplification Strategy.
摘要： MicroRNAs (miRNAs) play important roles in the regulation of target gene expression and cell development. Therefore, developing of accurate and visual detection methods for miRNAs is important for early diagnosis of cancer. In this study, we established a visual detection method for miRNA 155 based on DNAzyme amplification strategy in living cells. MnO2 nanosheets were employed to deliver Locked DNAzyme and Substrate DNA into cells. AuNPs-Probe were taken up by cells autonomously. Then, MnO2 nanosheets were reduced to Mn2+ by glutathione (GSH) in cells and DNA modules were released. MiRNA 155 took away Locker DNA by strand displacement reaction to activate the DNAzyme. Then the DNAzyme cleaved substrate DNA and released single-stranded DNA named Key DNA. Key DNA opened the hairpin DNA that modified on gold nanoparticles (AuNPs) and turn on the fluorescence of cy5. One target miRNA led to plenty of released Key DNA when lots of substrate DNA were added. Thus, the visual detection of miRNA 155 in living cells would be initiated. Under confocal laser microscopy, the fluorescence was obviously observed in tumor cells but not in normal cells. The method has a linear range from 0.1 nM to 10 nM and a low detection limit of 44 pM in vitro detection.
关键词： DNA walker,microRNA,AuNPs,Fluorescence imaging,MnO2,DNAzyme,Signal amplification
更新于2025-09-09 09:28:46