1：Experimental Design and Method Selection:

The study involved synthesizing self-enhanced Au-DPEA NCs to enhance ECL efficiency and constructing a multi-site landing DNA walker for signal amplification. The ECL aptasensor was designed for MUC1 detection.

2：Sample Selection and Data Sources:

MUC1 protein was used as the target, sourced from North Connaught Biotechnology Co., Ltd. Oligonucleotides were synthesized by Sangon Biological Engineering Technology and Services Co., Ltd.

3：List of Experimental Equipment and Materials:

Materials included HAuCl4·4H2O, NaBH4, DPEA, EDC, AgNO3, Fc-labeled quench probe, Exo III, and others from various suppliers. Equipment included MPI-A ECL analyzer, CHI 660C electrochemistry workstation, SEM (S-4800, Hitachi), HRTEM (FEI Tecnai G2 F20), UV-vis spectrophotometer (UV-2450, Shimadzu), and spectrofluorophotometer (F-5700, Hitachi).

4：Experimental Procedures and Operational Workflow:

Synthesis of Au-TA NCs and Au-DPEA NCs, preparation of DNA walker using Au@Fe3O4 nanoparticles and Exo III, assembly of the aptasensor on a GCE with layers of CS-Au-DPEA NCs, AgNPs, Fc-HP, and incubation with S2 and Exo III, followed by ECL measurement in PBS buffer.

5：Data Analysis Methods:

ECL intensities were measured and correlated with MUC1 concentrations using linear regression. Stability and selectivity were assessed with RSD calculations.