研究目的
To construct an ultrasensitive electrochemiluminescent (ECL) biosensor for the detection of microRNA-21 (miRNA-21) using a novel PTC-NH2/Lu nanocomposite as emitter and a bipedal DNA walker signal amplification strategy.
研究成果
The constructed biosensor, combining PTC-NH2/Lu nanocomposites as signal tags and a bipedal DNA walker for signal amplification, achieved ultrasensitive detection of miRNA-21 with a wide linear range and low detection limit. The biosensor demonstrated good performance in detecting miRNA-21 in lysates from human cancer cells, indicating its potential for biomedical analysis and clinical diagnostics.
研究不足
The study does not discuss the potential interference from other biomolecules in complex biological samples, nor does it address the long-term stability of the biosensor.
1：Experimental Design and Method Selection
The biosensor was designed based on the π-π stacking molecular assembly between luminol and PTC-NH2 for the immobilization of a large amount of luminol. PTC-NH2 acted as a novel co-reaction accelerator to enhance the ECL emission of the luminol-H2O2 system. A bipedal DNA walker strategy was employed for signal amplification.
2：Sample Selection and Data Sources
The target miRNA-21 was detected in lysates from human cancer cells (MCF-7 and HeLa).
3：List of Experimental Equipment and Materials
Perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA), 1,2-diaminoethane anhydrous (C2H8N2), luminol, N-(3-(dimethylamino) propyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), hexanethiol (HT), phosphate buffered solution (PBS), H2O2.
4：Experimental Procedures and Operational Workflow
1. Preparation of PTC-NH2/Lu nanocomposites via π-π stacking molecular assembly. 2. Preparation of signal probes by modifying the nanocomposites with label DNA. 3. Fabrication of the biosensor by immobilizing triple-stranded DNA on Au nanoflowers-modified glass carbon electrode. 4. ECL measurement in PBS containing H2O2.
5：Data Analysis Methods
The ECL intensity was measured and analyzed to determine the concentration of miRNA-21.
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