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A high-performance fluorescent probe for dopamine detection based on g-C3N4 nanofibers
摘要： A novel fluorescent sensor based on g-C3N4 nanofibers for the sensitive detection of dopamine (DA) has been proposed. We synthesized g-C3N4 nanofibers by directly hydrolyzing bulk g-C3N4 in the alkaline atmosphere (3 M NaOH). The obtained ultrathin g-C3N4 nanofibers were verified by characterizations of Transmission electronic microscope (TEM), X-ray diffractometer (XRD), Fourier transformation-infrared (FT-IR) and X-Ray photoelectron spectroscopy (XPS). It was found that the fluorescence intensity of g-C3N4 nanofibers was obviously quenched by DA. Fluorescence resonance energy transfer (FRET) between DA and g-C3N4 nanofibers led to the fluorescence reduction of g-C3N4 nanofibers. The fluorescent probe based on g-C3N4 nanofibers exhibits linear responses to the concentration of DA in the range from 0 to 4 μM and 4 to 20 μM, the limit of detection is 17 nM. The fluorescent probe shows excellent stability, good selectivity with its application in serums.
关键词： dopamine,g-C3N4 nanofibers,quenching,fluorescent probe
更新于2025-09-23 15:22:29
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A novel “turn-on” mitochondria-targeting near-infrared fluorescent probe for H2S detection and in living cells imaging
摘要： Hydrogen sulfide (H2S) has been considered to be involved in cytoprotective processes and redox signaling. It is very meaningful to track and analyze it in mitochondria. Herein, we report a novel “turn-on” mitochondria-targeting near-infrared fluorescent probe (Mito-NIR-SH) for detection of H2S in living cells, which was designed and synthesized by introducing 2,4-dinitrophenyl as fluorescence quenching group and H2S response moiety into Changsha near-infrared fluorophore (CS-OH). The structure of the fluorophore and the probe were characterized by 1H NMR, 13C NMR and mass spectrometry. Meanwhile, Mito-NIR-SH could quantitatively detect H2S at concentrations ranging from 0 to 30 μM with a detection limit as low as 89.3 nM, showing good chemical stability, fast “turn-on” response, selectively mitochondrial location, as well as high sensitivity and selectivity toward H2S. Based on this, it was successfully applied to imaging exogenous and endogenous H2S in living HeLa cells via confocal fluorescence microscopy.
关键词： High sensitivity and selectivity,Near-infrared fluorescent probe,Hydrogen sulfide,Mitochondria-targeting,Bioimaging
更新于2025-09-23 15:22:29
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An ESIPT-based fluorescent probe for Hg2+ in aqueous solution and its application in live-cell imaging
摘要： A new Excited-State Intramolecular Proton Transfer (ESIPT) based fluorescent probe for the detection of Hg2+ has been rationally designed and developed. Based on the specific reactivity of mercury-promoted hydrolysis, the probe exhibits high selectivity and sensitivity for mercury ions in almost pure aqueous solution (containing only 1% DMSO) with a low detection limit of 1.9 ppb. Furthermore, the probe was also successfully used for fluorescence imaging of Hg2+ in live cells.
关键词： Fluorescent probe,ESIPT-based,Live-cell imaging,Mercury ions,3-Hydroxyphthalimide
更新于2025-09-23 15:22:29
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A novel colorimetric and ratiometric fluorescent probe for cysteine based on conjugate addition-cyclization-elimination strategy with a large Stokes shift and bioimaging in living cells
摘要： Based on conjugate addition-cyclization reaction of Cys with acrylate and subsequent 1,6-elimination of p-hydroxybenzyl moiety, a novel colorimetric and ratiometric fluorescent probe 1 was designed and synthesized. Upon addition of Cys to the solution of 1, the absorption spectra changed from 508 nm to 452 nm (?56 nm) and afforded visible color change from pink to yellow. Meanwhile, the emission spectra shifted from 644 nm to 539 nm (?105 nm) with remarkable changes in the emission ratio of F539 nm/F644 nm (R/R0 up to 760-fold), accompanying with an obvious fluorescence change from orange to green under illumination with a 365 nm UV lamp. In addition, 1 exhibited a large Stokes shift (136 nm), high sensitivity (detection limit of 46.7 nM), and excellent selectivity to Cys over Hcy and GSH. Moreover, 1 can discriminate Cys from Hcy and GSH by fluorescence spectra, even obvious visible and fluorescence color changes. Importantly, 1 can be used to image Cys in living cells by dual emission channels.
关键词： Fluorescent probe,Cysteine,Ratiometric,Bioimaging,Colorimetric
更新于2025-09-23 15:22:29
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A rhodamine-deoxylactam based fluorescent probe for fast and selective detection of nitric oxide in living cells
摘要： Nitric oxide (NO) plays vital roles in many physiological process and is closely related to many diseases. So far, a number of fluorescent probes have been constructed for the detection of NO successfully. However, the probes still suffer from long-time reaction and limited selectivity. Herein, a fluorescent probe named dRB-OPD is synthesized and used to recognize NO. The probe contains a deoxy-rhodamine B as fluorophore and o-phenylenediamino as reaction site. dRB-OPD shows fast response to NO within 40 seconds with 170-fold fluorescence enhancement. Moreover, the probe shows high selectivity towards NO over dehydroascorbic acid (DHA), ascorbic acid (AA), and methylglyoxal (MGO). Particularly, the probe can avoid the serious interference from cysteine (Cys) found in the rhodamine lactam-based fluorescent NO probes (RB-OPD). In addition, the probe is applied for the detection of exogenous and endogenous NO in the HepG2 and RAW 264.7 cells with satisfactory results.
关键词： Cell imaging,Fluorescent probe,Nitric oxide,High selectivity,Fast response
更新于2025-09-23 15:22:29
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Hydrazine Exposé: The Next-Generation Fluorescent Probe
摘要： Hydrazine (N2H4) is one of the most important pnictogen hydride chemicals, and is utilized within a wide spectrum of industries. As a result of its extensive use, hydrazine’s monitoring methods have constantly come under fire due to its potential health risk and the subsequent environmental pollution. Fluorometric molecular sensing systems generally report with a major emphasis on the merit of fluorescence analysis. What we are proposing within this report is a next-generation fluorescent probe that allows hydrazine to become fully traceable, within multifarious environments that show fast and intuitional fluorescence transformation. A new sensing moiety, ortho-methoxy-methyl-ether (o-OMOM) incorporated electron donor (D)–acceptor (A) type naphthaldehyde provides high selectivity and sensitivity amidst its superiority within practical applications for sensing hydrazine. The new probe overcomes most of the drawbacks of currently used fluorescent probes, and due to its successful demonstrations, such as real-time spray-based sensing, soil analysis, and two-photon tissue imaging, its potential for practical application is beyond reproach.
关键词： fluorescent probe,chemical sensor,hydrazine,hydrazone-formation,two-photon tissue imaging
更新于2025-09-23 15:22:29
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A novel dicationic Quinoline-Carzole fluorescent probe：Preparation and labeling of <i>Microthrix parvicella</i>
摘要： A novel dicationic Quinoline-Carbazole fluorescent probe with hydrophobic long-chain alkane was designed and synthesized based on the property of Microthrix parvicella (M. parvicella) in situ utilizing long-chain fatty acids (LCFA) in the activated sludge system. 1H NMR spectrum, ultraviolet-visible (UV-Vis) absorption spectra, and fluorescent spectra analysis demonstrated that the probe was successfully obtained. The probe had a large stokes-shift ranging from 102 to 144nm in different solvents, which were benefit for the fluorescent labeling properties. The labeling experiment indicated that the prepared probe could absorb onto the surface of M. parvicella through hydrophobic bond. Much stronger yellow fluorescence of M. parvicella was observed at the concentration of 1.0×10-5mol/L when compared with the zooglea, which makes it easy to distinguish M. parvicella from the zooglea. In addition, the photostability of the probe was also investigated, and the result showed that the probe was quite stable in a long period of time. All the results indicated that the prepared probe was suitable for the labeling of M. parvicella.
关键词： Dicationic Quinoline-Carbazole,fluorescent probe,Characterization,labeling,Microthrix parvicella
更新于2025-09-23 15:22:29
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A fluorescent turn-on probe for Hg2+ with a high contrast designed by manipulating functional groups tethered to naphthalimide
摘要： Based on the concept provided by our previous work on a fluorescent Cd2+ probe, a fluorescent turn-on Hg2+ probe with a high contrast was designed through manipulating functional groups tethered to naphthalimide. With carbamoylmethylamino and bis[2-(ethylthio)ethyl]amino groups as tethering functionalities, 1 worked as a useful fluorescent probe in a high selective and sensitive manner over the pH range between 6 and 8 with a detection limit at 2.8 nM (estimated at a pH of 7.2). Comparison of the probe performance of 1 with that of 2 merely with bis[2-(ethylthio)ethyl]amino group suggested that both of the tethering groups exhibited their abilities to chelate with Hg2+, and yet the carbamoylmethylamino group also functioned as a fluorescent off-on switch through a photo-induced electron transfer mechanism. Thus, 1 found a practical utility as an Hg2+ probe in analysis of river as well as tap samples without any tedious pretreatments.
关键词： Carbamoylmethylamino group,Fluorescent probe,Naphthalimide,Fluorescence,Mercury
更新于2025-09-23 15:21:21
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Selective and Sensitive Sensing of Hydrogen Peroxide by a Boronic Acid Functionalized Metal–Organic Framework and Its Application in Live-Cell Imaging
摘要： A new boronic acid functionalized Zr(IV) metal?organic framework having the capability of sensing H2O2 in live cells is reported. The Zr-MOF bears a UiO-66 structure and contains 2-boronobenzene-1,4-dicarboxylic acid (BDC-B(OH)2) as a framework linker. The activated Zr-UiO-66-B(OH)2 compound (called 1′) is highly selective for the ?uorogenic detection of H2O2 in HEPES bu?er at pH 7.4, even in the presence of interfering ROS (ROS = reactive oxygen species) and other biologically relevant analytes. The ?uorescent probe was found to display extraordinary sensitivity for H2O2 (detection limit 0.015 μM) in HEPES bu?er, which represents a lower value in comparison to those of the MOF probes documented so far for sensing H2O2 using other analytical methods. Taking advantage of its high selectivity and sensitivity for H2O2 in HEPES bu?er, the probe was successfully employed for the imaging of intracellular H2O2. Imaging studies with MDAMB-231 cells revealed the emergence of bright blue ?uorescence after loading with probe 1′ and subsequent treatment with H2O2 solution.
关键词： Zr(IV) metal?organic framework,boronic acid,live-cell imaging,fluorescent probe,H2O2 sensing
更新于2025-09-23 15:21:21
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Design, synthesis, crystal structure and cytotoxicity studies of colorimetric fluorescent “OFF-ON” probes for rapid detection of hydrogen sulfide based on Cu(II) complex
摘要： Four colorimetric fluorescent probes based on copper(II) complexes were synthesized and the molecular structures were characterized by X-ray diffraction, ESI-HRMS and elemental analysis. When exposed to HS–, the synthesized probes showed significant color changes from yellow to red and remarkable increase of fluorescence intensity (over 80-fold). What’s more, the interaction of host-guest could be completed in 2 minutes. However, no clear color and fluorescence intensity changes were observed in the presence of other anions (AcO–, H2PO4–, F–, Cl–, Br–, I–, SO42-, SO32-, CO32-, Cys and GSH). The synthesized colorimetric fluorescent probes could detect HS– rapidly and conveniently. Cytotoxicity studies indicated that the synthesized fluorescent probes were low cytotoxicity in Hela cells and may be used to detect H2S level in vivo.
关键词： hydrogen sulfide,copper complex crystal,fluorescent probe,colorimetric detection,cytotoxicity
更新于2025-09-23 15:21:01