研究目的
To design and synthesize a novel dicationic Quinoline-Carbazole fluorescent probe for the identification and labeling of Microthrix parvicella in activated sludge systems to address sludge bulking issues.
研究成果
The synthesized dicationic Quinoline-Carbazole fluorescent probe has large stokes shift, good photostability, and effectively labels M. parvicella in activated sludge through hydrophobic interactions, with an optimal concentration of 1.0×10-5 mol/L. It is suitable for identifying M. parvicella to address sludge bulking, but future work should focus on improving solubility and quantum yield.
研究不足
The probe has low fluorescence quantum yield in water, which may restrict its applications; further optimization is needed to balance hydrophobicity and solubility in activated sludge samples.
1：Experimental Design and Method Selection:
The study involved designing a fluorescent probe based on the hydrophobic properties of M. parvicella, synthesizing it via a chemical route involving compounds 2 and 3 under piperidine catalysis, and characterizing it using UV-Vis, fluorescence spectra, and 1H NMR.
2：Sample Selection and Data Sources:
Activated sludge samples were collected from WWTPs in Tianjin, China, stored at 4°C, and diluted to 1g MLSS/L for experiments.
3：List of Experimental Equipment and Materials:
Equipment included UV-Visible spectrophotometer (UV-2600, Shimadzu), Hitachi F-7000 spectrometer, Bruker 400 MHz NMR spectrometer, inverted fluorescence microscope (Olympus IX71), and iodine-tungsten lamp (500W). Materials included ethanol, methanol, DMSO, DCM, DMF, carbazole, 1-Bromooctane, 4-methylquinoline, 3-bromo-propylamine hydrobromide, piperidine, rhodamine B, and activated sludge samples.
4：Experimental Procedures and Operational Workflow:
Synthesis of probe, determination of fluorescent quantum yields using rhodamine B as reference, photostability testing under light irradiation, and labeling experiments by adding probe to sludge samples and observing under microscope.
5：Data Analysis Methods:
Fluorescent quantum yields calculated using a standard equation, spectra analyzed for stokes shift and intensity, and microscopic images evaluated for labeling efficiency.
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