- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Organic semiconducting nanoprobe with redox-activatable NIR-II fluorescence for <i>in vivo</i> real-time monitoring of drug toxicity
摘要： An activatable organic semiconducting nanoprobe that specifically turns on its second near-infrared window fluorescence upon being exposed to nitric oxide stimuli was developed for in vivo, in situ, real-time and high-spatial-resolution mapping of drug-induced hepatotoxicity.
关键词： drug toxicity,nitric oxide,NIR-II fluorescence,hepatotoxicity,redox-activatable,in vivo monitoring,Organic semiconducting nanoprobe
更新于2025-09-19 17:15:36
-
Single-Cell ATP Content Monitoring during Hyperthermia Cell Death by using Plasmonic Fluorescent Nanoflare
摘要： Gold nanorods-based plasmonic photothermal therapy (AuNRs-PPTT) has been a prospective anti-cancer approach in which AuNRs absorbs near-infrared (NIR) light and converts it into heat, leading to cell death. Investigating molecular energy metabolism of single cells, especially cancer cells, during hyperthermia cell death process is therefore of great significance, as it can help us to better understand the photothermal lethal mechanism of cancer cells and design new photothermal probes more rationally. However, during the AuNRs-PPTT process, how the cells respond to heat stimulation, and how their energy metabolism changes, these basic issues have rarely been studied. Herein, we selected adenosine triphosphate (ATP) as a target molecule, and by preparing a plasmonic and turn-on type fluorescent nanoprobe we examined the ATP metabolism difference between cancerous cells and normal cells during the AuNRs-PPTT process. We found that the fluorescence intensity increased ~ 60% after 5 min laser irradiation as compared to the initial intensity in single HeLa cells, but only ~ 20% increasement was observed for single H8 cells; obviously the increase of ATP content in cancerous cells was notably higher than that in normal cells during the hyperthermia cell death.
关键词： Plasmonic photothermal therapy,Fluorescent nanoprobe,ATP,Gold nanorods,Hyperthermia cell death
更新于2025-09-19 17:13:59
-
An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
摘要： Ketamine is a commonly abused drug due to its stimulant, dissociative and hallucinogenic effects. An overdose of ketamine has been found to cause a variety of side effects. Therefore, the identification and quantification of ketamine are of significant importance for clinical purposes and drug seizing. However, conventional methods for ketamine detection possess some disadvantages such as sophisticated procedures, expensive instruments and low sensitivity. Herein, we develop a novel fluorescent nanoprobe for ultrasensitive ketamine detection with signal amplification based on Adenosine Triphosphate (ATP)-fueled target recycling and FRET (fluorescence resonance energy transfer) occurring between the FAM (Fluorescein, tagged with Y-shape DNA) and AuNPs. Based on the combination of FRET and signals circle amplification, the gold nanospheres functionalized with Y-motif DNA (Y@AuNPs) nanoprobe was utilized for effective ketamine detection with the limit of detection (LOD) down to 3 pg mL?1, which was lower than previously reported. Furthermore, the high sensitivity of Y@AuNPs facilitated quantitative analysis in biological media and practical samples.
关键词： Ultrasensitive detection,Ketamine,FRET,ATP-fueled target recycling,Fluorescent nanoprobe
更新于2025-09-16 10:30:52
-
A new ratiometric fluorescence assay based on resonance energy transfer between biomass quantum dots and organic dye for the detection of sulfur dioxide derivatives
摘要： Sulfur dioxide (SO2) is considered as the fourth gas signal molecule after nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). It plays important roles in several physiological processes. Therefore, the design and synthesis of nanoprobes for the detection of SO2 derivatives in cells is of great significance. Herein, we report a new ratiometric fluorescence nanoprobe based on resonance energy transfer (RET) between biomass quantum dots (BQDs) and organic dye (DMI) for the detection of SO2 derivatives. The proposed ratiometric fluorescence assay allows the determination of HSO3? in the range of 1.0 to 225 μM with a detection limit of 0.5 μM. Importantly, the proposed ratiometric fluorescence nanoprobe exhibits a high photostability and good selectivity for HSO3? over other chemical species including H2S and biological mercaptans. Quantitation of HSO3? in cell lysates by using the nanoprobe is demonstrated.
关键词： ratiometric fluorescence,resonance energy transfer,organic dye,Sulfur dioxide,biomass quantum dots,nanoprobe
更新于2025-09-16 10:30:52
-
Single Component Self-Assembled Thermally Activated Delayed Fluorescence Nanoprobe
摘要： A novel versatile thermally activated delayed fluorescence (TADF) nanoprobe, AI-Cz-NP, was designed and fabricated through self-assembly of the single-component amphiphilic monomer for potential applications in confocal imaging and time-resolved fluorescence imaging.
关键词： nanoprobe,self-assembly,thermally activated delayed fluorescence,confocal imaging,time-resolved fluorescence imaging
更新于2025-09-16 10:30:52
-
A turn-on graphene quantum dot and graphene oxide based fluorometric aptasensor for the determination of telomerase activity
摘要： A turn-on fluorometric assay is described for determination of the activity of enzyme telomerase. For this purpose, graphene quantum dots (GQDs) were first modified with the telomeric sequence (5′-amino-AATCCGTCGAGCAGAGTT-3′) via a condensation reaction. Injection of graphene oxide causes instant quenching of the blue fluorescence of the GQDs. Addition of cell extract containing telomerase, triggers the extension of telomer via addition of specific sequence (TTAGGG)n to its 3′ end. Fluorescence, best measured at excitation/emission wavelengths of 390/446 nm, is subsequently restored due to folding of the extended telomeric sequence into G-quadruplex structure. The method was applied to the determination of telomerase activity in crude cell extracts of as little as 10 HeLa cells. The linear dynamic range extends from 10 to 6500 cells.
关键词： G-quadruplex,Biosensors,Fluorescence,Optical sensing,Biomarker,Cancer detection,Nanoprobe
更新于2025-09-12 10:27:22
-
Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher
摘要： A fluorometric aptamer based assay is described for rapid and sensitive detection of aflatoxin B1 (AFB1). It is making use of a fluorescein (FAM) labeled anti-AFB1 aptamer and complementary DNA-modified gold nanoparticles (GNPs). In the absence of AFB1, the FAM-labeled aptamers hybridize with complementary DNA strands that were covalently immobilized on GNPs. This results in quenching of the green fluorescence (with excitation/emission peaks at 485/525 nm). In the presence of AFB1, the aptamer probe binds AFB1 and is released from the GNPs. Hence, fluorescence is restored. Under optimized conditions, AFB1 in the concentration range from 61 pM to 4.0 μM can be detected, and the detection limit is 61 pM. This assay is highly selective for AFB1. It was applied to the determination of AFB1 spiked into 50-fold diluted wine and 20-fold diluted beer.
关键词： Mycotoxin,Food safety,Environmental analysis,Fluorescence quenching,Fluorophore,Fluorescent probe,Nanomaterials,Nanoprobe
更新于2025-09-11 14:15:04
-
Fluorescence-SERS dual-signal probes for pH sensing in live cells
摘要： The activity of many cells is closely related to the pH of their internal environment, and the increase of intracellular pH value is a common feature of many drug-resistant tumors. The intracellular pH needs to be determined as the research basis when studying the intracellular transport protein and ion concentration changes. Nanomaterials are now widely used as the effective imaging and drug delivery vehicles, but it remains to be studied as a sensor for intracellular environment. Based on this, we have constructed a nanoprobe with fluorescence and surface-enhanced Raman scattering (SERS) dual-signal for sensing intracellular pH. The principle of this strategy is to construct fluorescence-SERS dual-signal nanoprobes by modifying pH-responsive fluorescent probes and SERS reporter molecules on the surface of gold nanorods (AuNRs) with the core-shell structure. The fluorescence-SERS dual response of the nanoprobe to pH can be achieved by investigating the fluorescence and SERS spectra of nanoprobes at different pH. Moreover, by incubating the nanoprobes into different cells, different double-signal response results can be obtained, thereby achieving intracellular pH sensing. The nanoprobe has dual responsiveness to fluorescence and SERS, which makes up for many of the deficiencies of single-signal probes, and realizes high sensitivity, accuracy and stability of intracellular pH detection. It is expected to be widely applied in the fields of medicine, chemistry and biology.
关键词： Dual-signal,Intracellular pH,Fluorescence,Surface-enhanced Raman scattering,Nanoprobe
更新于2025-09-10 09:29:36
-
Developing the XEOL and TR-XEOL at the X-ray Nanoprobe at Taiwan Photon Source
摘要： The hard X-ray excited optical luminescence (XEOL) and time-resolved X-ray excited optical luminescence (TR-XEOL) have been developed successfully at the X-ray Nanoprobe (XNP) facility at Taiwan Photon Source (TPS). Not only the XNP at TPS provides 40 nm spatial resolution, but also the X-ray energy of synchrotron source is continuous and tunable, which make the XEOL and TR-XEOL are powerful tool to study the optical properties and dynamic luminescence of novel optoelectronic materials.
关键词： optoelectronic materials,XEOL,X-ray Nanoprobe,TR-XEOL,Taiwan Photon Source
更新于2025-09-10 09:29:36
-
Aluminum(III) triggered aggregation-induced emission of glutathione-capped copper nanoclusters as a fluorescent probe for creatinine
摘要： Glutathione-capped copper nanoclusters (CuNCs) are presented that display aggregation-induced emission (AIE). This feature was exploited for selective and sensitive quantification of creatinine (CRN) which is an important diagnostic parameter. In the presence of Al3+ ions, such CuNCs rapidly aggregate, and this induces enhanced a red emission. The AIE nature of CuNCs was proven via TEM and fluorimetry. On addition of CRN, the coordination between CRN and Al3+ ions led to the quenching of fluorescence due to weakening the AIE. The best fluorescence intensity was measured at excitation/emission peaks of 360/585 nm. Quenched fluorescence intensity showed a linear dependence on the concentrations of CRN in the range of 2.5–34 μgL?1 with a detection limit of 0.63 μgL?1. The sensing mechanism of probe for CRN detection is discussed. The probe was applied to the determination of CRN in spiked human serum samples and gave satisfactory results.
关键词： Nanosensor,Renal biomarker,Serum analysis,Fluorescent nanomaterials,Metal nanoclusters,Nanoprobe,Paired t-test,Fluorometry,Real sample analysis
更新于2025-09-04 15:30:14