研究目的
Investigating the therapeutic effects of a specific herbal medicine on a particular disease.
研究成果
The developed assay is a simple, cost-effective method for sensitive, specific, and reliable quantitative analysis of telomerase activity in cancerous cells, with potential for further in-vivo studies.
研究不足
The study is limited by the specificity and sensitivity of the fluorometric assay, potential interference from other cellular components, and the need for further validation in clinical samples.
1：Experimental Design and Method Selection:
The study developed a turn-on fluorescence assay exploiting the strong and highly stable fluorescence of GQDs, high quenching efficiency of GO, and their excellent donor–acceptor pairing. The ability of the elongated telomeric sequence to form a G-quadruplex structure was also utilized.
2：Sample Selection and Data Sources:
Human cancer cell lines (HeLa, A549, MCF-7) and a normal human lung fibroblast cell line (HLF) were used.
3：List of Experimental Equipment and Materials:
Graphene quantum dots (GQDs), graphene oxide (GO), telomeric sequence, cell culture materials, and various chemicals were used.
4：Experimental Procedures and Operational Workflow:
GQDs were modified with the telomeric sequence, GO was added to quench fluorescence, and telomerase activity was measured by fluorescence restoration.
5：Data Analysis Methods:
Fluorescence emission spectra were recorded, and signal output was calculated based on fluorescence intensities.
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