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- 摘要
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- 实验方案
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Multifunctional nanoclusters of NaYF4:Yb3+,Er3+ upconversion nanoparticle and gold nanorod for simultaneous imaging and targeted chemotherapy of bladder cancer
摘要： This paper reports successful synthesis of multifunctional nanoclusters of upconversion nanoparticle (UCNP) and gold nanorod (AuNR) through a PEGylation process. UCNPs emit visible luminescence under near-infrared excitation, producing high-contrast images with no background fluorescence. When coupled with AuNRs, the resulting UCNP-AuNR multifunctional nanoclusters are capable of simultaneous detection and treatment of bladder cancer. These UCNP-AuNR nanoclusters are further functionalized with antibodies to epidermal growth factor receptor (EGFR) to target bladder cancer cells known to overexpress EGFRs. This paper demonstrates, for the first time, efficient targeting of bladder cancer cells with UCNP-AuNR nanoclusters. In addition to high-contrast imaging and consequently high sensitivity detection of bladder cancer cells, highly selective optoporation-assisted chemotherapy was accomplished using a dosage of chemotherapy agent significantly lower than any previous reports, within a clinically relevant incubation time window. These results are highly relevant to the eventual human application in which the nanoclusters and chemotherapy drugs will be directly instilled in bladder via urinary catheter.
关键词： Luminescence upconversion,Surface Plasmon,Bladder Cancer,Gold Nanorod,Optoporation
更新于2025-09-23 15:23:52
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[IEEE 2018 International Symposium on Micro-NanoMechatronics and Human Science (MHS) - Nagoya, Japan (2018.12.9-2018.12.12)] 2018 International Symposium on Micro-NanoMechatronics and Human Science (MHS) - Uniform Transfection: Shock Wave Generation in Laser Ablation and Microcontact Printing
摘要： With the increasing need of regenerative medicines, cell therapy, and biopharmaceutical drug, it is necessary to develop a method for massively parallel delivery and uniform transfection. This research explores the possibility of using μCP (using cell adhesive and cell blocking agents) for printing massive number of cells on a substrate and using optoporation methods (nano-second pulsed radiation) to perforate cell membranes. With combining these two methods, a stand-alone micro-device capable of performing localized intracellular delivery will be developed. In this paper, we confirmed the generation of shock wave upon optoporation, tried intra-cellular delivery using Calcein, develop the stamp for micro-contact printing and performed the printing.
关键词： optoporation,laser ablation,uniform transfection,shock wave generation,microcontact printing
更新于2025-09-19 17:13:59