研究目的
To develop multifunctional nanoclusters for simultaneous imaging and targeted chemotherapy of bladder cancer using upconversion nanoparticles and gold nanorods.
研究成果
The UCNP-AuNR nanoclusters enable efficient targeting, high-contrast imaging, and selective optoporation-assisted chemotherapy with reduced drug dosage and incubation time, showing promise for clinical bladder cancer treatment.
研究不足
The yield of successful conjugation was 34%, which could be improved. The study is in vitro and may not fully replicate in vivo conditions. Laser parameters and cell-specific responses may require optimization for clinical application.
1：Experimental Design and Method Selection:
The study involved synthesizing UCNPs via thermal decomposition and AuNRs via seed-mediated growth, followed by PEGylation to create UCNP-AuNR nanoclusters. These were functionalized with anti-EGFR antibodies for targeted binding. Methods included SEM, TEM, confocal microscopy, darkfield imaging, and optoporation with femtosecond laser irradiation.
2：Sample Selection and Data Sources:
Human bladder cancer cell lines (A549, H520, HTB9, T24T) were used, selected based on EGFR expression. Data were acquired through microscopy and spectroscopy.
3：List of Experimental Equipment and Materials:
Equipment included Zeiss LSM 780 confocal microscope, Ti:Sapphire laser, SEM, TEM. Materials included UCNPs, AuNRs, PEG, antibodies, cisplatin, propidium iodide.
4：Experimental Procedures and Operational Workflow:
Cells were incubated with nanoclusters for 2h, washed, irradiated with laser, and imaged. Optoporation was performed with PI or cisplatin, and cell viability was assessed.
5：Data Analysis Methods:
Data were analyzed using microscopy image analysis, PL spectroscopy, and statistical methods for cell viability quantification.
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Zeiss LSM 780 confocal microscope
LSM 780
Zeiss
Used for upconversion imaging and confocal microscopy of cells.
ZEISS LSM 990 Spectral Multiplex
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Ti:Sapphire laser
Used for femtosecond pulsed laser irradiation at 800 nm for optoporation.
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SEM
Used for scanning electron microscopy to examine nanocluster morphology.
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TEM
Used for transmission electron microscopy to examine nanocluster structure.
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Darkfield microscope
Used for NIR darkfield imaging to detect AuNR scattering.
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