研究目的
To develop a method for massively parallel delivery and uniform transfection combining micro-contact printing with laser ablation to achieve localized intracellular delivery with high efficiency and cell viability.
研究成果
PDMS stamp fabrication was successful, but improvements in μCP technique are needed. Shock wave generation was confirmed via bubble formation during laser ablation. Future work includes improving μCP with a bigger diameter SU-8 mold and confirming intracellular delivery with non-permeable dyes.
研究不足
The adhesive agent used in μCP has more affinity to the PDMS stamp than to the glass substrate, leading to loss of fluorescein after stamp removal. Calcein's permeability to cells made it unsuitable for confirming intracellular delivery.
1：Experimental Design and Method Selection:
The study combines cell adhesion techniques (μCP) with optoporation methods (nano-second pulsed radiation) for intracellular delivery.
2：Sample Selection and Data Sources:
HeLa cells and Calcein solution were used for intracellular delivery experiments.
3：List of Experimental Equipment and Materials:
SU-8 3005, PDMS, Titanium (Ti), Piranha solution, Fluorescein, Calcein, ns-pulsed laser.
4：Experimental Procedures and Operational Workflow:
Includes SPM cleaning, SU8 mold preparation, silanization, PDMS casting, fluorescein preparation, stamping, substrate preparation for laser ablation, and optoporation experiments.
5：Data Analysis Methods:
Observation of shock wave generation and bubble formation, fluorescence imaging for stamping results.
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