- 标题
- 摘要
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- 实验方案
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PEGylated-folic acida??modified black phosphorus quantum dots as near-infrared agents for dual-modality imaging-guided selective cancer cell destruction
摘要： Biological systems have high transparence to 700–1100-nm near-infrared (NIR) light. Black phosphorus quantum dots (BPQDs) have high optical absorbance in this spectrum. This optical property of BPQDs integrates both diagnostic and therapeutic functions together in an all-in-one processing system in cancer theranostic approaches. In the present study, BPQDs were synthesized and functionalized by targeting moieties (PEG-NH2-FA) and were further loaded with anticancer drugs (doxorubicin) for photodynamic–photothermal–chemotherapy. The precise killing of cancer cells was achieved by linking BPQDs with folate moiety (folic acid), internalizing BPQDs inside cancer cells with folate receptors and NIR triggering, without affecting the receptor-free cells. These in vitro experiments confirm that the agent exhibited an efficient photokilling effect and a light-triggered and heat-induced drug delivery at the precise tumor sites. Furthermore, the nanoplatform has good biocompatibility and effectively obliterates tumors in nude mice, showing no noticeable damages to noncancer tissues. Importantly, this nanoplatform can inhibit tumor growth through visualized synergistic treatment and photoacoustic and photothermal imaging. The present design of versatile nanoplatforms can allow for the adjustment of nanoplatforms for good treatment efficacy and multiplexed imaging, providing an innovation for targeted tumor treatment.
关键词： drug release,black phosphorus quantum dots,synergistic therapy,targeted,photoacoustic imaging
更新于2025-09-19 17:13:59
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Self-Assembled Carrier-Free Nanosonosensitizer for Photoacoustic Imaging-Guided Synergistic Chemo-Sonodynamic Cancer Therapy
摘要： As one of the most promising noninvasive therapeutic modalities, sonodynamic therapy (SDT) can focus the ultrasound energy on tumor sites located in deep tissue and locally activate the preloaded sonosensitizer to kill tumor cells. However, exploring sonosensitizers with high SDT efficacy and desirable biosafety is still a significant challenge. Herein, we utilized the hydrophilic-hydrophobic self-assembly technology to assemble the hydrophobic organic dye Ce6 and broad spectral anti-cancer agent Paclitaxel with hydrophilic organic dye IR783 to generate nanoscale sonosensitizer, Ce6-PTX@IR783, without introduction of extra nanomaterials into the fabrication to guarantee the high therapeutic biosafety and further potential clinical translation. The constructed nanodrug was endowed with external ultrasound-activatable chemo-sonodynamic effect and photoacoustic imaging performance via integrating multiple moieties into one nanosystem. Ce6 could enhance the sonodynamic effect, while PTX exerted chemotherapeutic effect, and IR783 was applied to increase tumor-specific accumulation and assist in fulfilling photoacoustic imaging. Especially, the small particle size (70 nm) of Ce6-PTX@IR783 contributed to the increased tumor accumulation via the enhanced permeability and retention effect. The high synergistically chemo-sonodynamic therapeutic efficacy has been successfully demonstrated in vitro and in vivo, in addition to the demonstrated high biodegradability, biocompatibility and biosafety. This facile self-assembly procedure provides an intriguing strategy for high-efficient utilization of hydrophobic drugs and is liable to realize large-scale production and further clinical translation.
关键词： self-assembly,nanosonosensitizer,photoacoustic imaging,chemo-sonodynamic therapy,sonodynamic therapy
更新于2025-09-19 17:13:59
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[IEEE TENCON 2019 - 2019 IEEE Region 10 Conference (TENCON) - Kochi, India (2019.10.17-2019.10.20)] TENCON 2019 - 2019 IEEE Region 10 Conference (TENCON) - Point-of-care functional and molecular imaging using LED-based photoacoustics
摘要： Photoacoustic (PA) or optoacoustic imaging can visualize tissue-optical absorbers, especially hemoglobin, with optical contrast and ultrasound (US)-like resolution and imaging depth. Since both PA and US imaging involves US detection, it is straightforward to develop dual-mode imaging systems with unprecedented functional and structural imaging capabilities. Researchers have already demonstrated the potential of utilizing this complementary contrast imaging for several animal imaging experiments and early clinical pilot studies. PA conventionally uses slow, bulky and high-priced lasers as excitation sources. Use of these high-power pulsed lasers is hindering the clinical translation process of this imaging modality with tremendous potential. Advances in solid-state device technology have recently resulted in the development of a new class of high-power light emitting diodes (LEDs) that can be used as fast, robust and affordable pulsed excitation sources for PA imaging. In this paper, we review multiple LED-based PA/US imaging implementations (commercial and lab-made systems) and demonstrate its functional, molecular and structural imaging capabilities using several clinical/preclinical imaging examples. Specific focus will be given to 2D and 3D superficial vasculature and oxygen saturation imaging in multiple in vivo clinical and preclinical studies.
关键词： ultrasonography,vasculature,photoacoustic imaging,oxygenation imaging,LED
更新于2025-09-16 10:30:52
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Self-Assembled Polysaccharide–Diphenylalanine/Au Nanospheres for Photothermal Therapy and Photoacoustic Imaging
摘要： Gold-based nanomaterials have attracted extensive interest for potential application in photothermal therapy (PTT) owing to their distinctive properties including high photothermal transduction, biocompatibility, and low cytotoxicity. Herein, assembled gold nanoparticle architecture-based photothermal conversion agents were synthesized by using polysaccharides (alginate dialdehyde, ADA) as both the cross-linker to induce self-assembly of diphenylalanine (FF) and the reducer for in situ reduction of Au3+ ions into Au nanoparticles (Au NPs). The extinction spectrum of the obtained self-assembled ADA?FF/Au nanospheres was finely modulated into a near-infrared region by controlling the growth of Au NPs inside the assemblies. The strong plasmonic coupling effect of the assembled Au NPs also leads to high photothermal conversion (η = 40%) of the ADA?FF/Au nanospheres, hence presenting good performance in PTT and photoacoustic imaging. This synthesis technique is promising to construct nanomaterials with desired functions for potential biomedical application by self-assembly of various nanocrystals in situ.
关键词： self-assembly,photoacoustic imaging,photothermal therapy,biomedical application,gold-based nanomaterials
更新于2025-09-16 10:30:52
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Effects of freezing on mesenchymal stem cells labeled with gold nanoparticles
摘要： Stem cell therapies are a promising treatment for many patients suffering from diseases with poor prognosis. However, clinical translation is inhibited by a lack of in vivo monitoring techniques to track stem cells throughout the course of treatment. Ultrasound-guided photoacoustic imaging of nanoparticle-labeled stem cells may be a solution. To allow photoacoustic tracking, stem cells must be labeled with an optically absorbing contrast agent. Gold nanoparticles are one option due to their cytocompatibility and strong optical absorption in the near-infrared region. However, stem cell labeling can require up to 24-hour incubation with nanoparticles in culture prior to use. Although stem cell monitoring is critically needed, the additional preparation time may not be feasible – it is cost prohibitive and stem cell treatments should be readily available in emergency situations as well as scheduled procedures. To remedy this, stem cells can be labeled prior to freezing and long-term storage. While it is well known that stem cells retain their cellular function after freezing, storage, and thawing, the impact of gold nanoparticles on this process has yet to be investigated. Therefore, we assessed the viability, multipotency, and photoacoustic activity of gold nanosphere-labeled mesenchymal stem cells after freezing, storage, and thawing for one week, one month, or two months and compared to unlabeled, na?ve mesenchymal stem cells which were frozen, stored, and thawed at the same time points. Results indicated no substantial change in viability as assessed by the MTT assay. Differentiation, observed through adipogenesis and osteogenesis, was also comparable to controls. Lastly, strong photoacoustic signals and similar photoacoustic spectral signatures remained. Further studies involving more diverse stem cell types and nanoparticles are required, but our data suggests that function and imaging properties of nanoparticle-labeled stem cells are maintained after freezing and storage, which improves translation of stem cell monitoring techniques by simplifying integration with clinical protocols.
关键词： photoacoustic imaging,cell tracking,freezing,Gold nanospheres,ultrasound,stem cells
更新于2025-09-16 10:30:52
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Photoacoustic Imaging Quantifies Drug Release from Nanocarriers via Redox Chemistry of Dye‐Labeled Cargo
摘要： There have been remarkable advances in imaging drug nanocarriers, but there are few real-time imaging strategies to determine if the cargo has been released from the carrier. This is important because the pharmacokinetics and pharmacodynamics of the carrier can often be dramatically decoupled from that of the cargo. Thus, new tools are clearly needed to image the timing and quantity of drug release from nanocarriers. Here, we describe a simple strategy for photoacoustic monitoring of drug release based on the redox chemistry of methylene blue, which offers predictable redox chemistry: It can transition from the oxidized state with a bright blue color and robust photoacoustic signal to the reduced state that the transparent with no photoacoustic signal. We locked this drug-dye conjugate into a reduced state inside of a nanoparticle with no photoacoustic signal. As the drug is released from the carrier, the dye is oxidized for quantification with photoacoustic imaging. We first prepared paclitaxel-methylene blue conjugate (PTX-MB) with strong absorbance at 640 nm and photoacoustic intensity proportional to its concentration. This cargo was co-encapsulated in a poly(lactic-co-glycolic acid) nanoparticle with a dithiothreitol reducing agent. The IC50 of PTX-MB-loaded NPs (PTX-MB @ PLGA NPs) was 78 μg mL-1. We then used the redox reaction of PTX-MB to monitor its release from poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). In vitro drug-release in phosphate buffer saline with 20% v/v normal mice serum showed a 670-fold increase in photoacoustic signal. The particles showed an initial burst release (25%) during the first 24 hours. After 24 hours, a sustained release was observed through 120 hours leading to cumulative release of 40.6% of PTX-MB. In vivo drug release study in mice for a duration of 12 hours showed a photoacoustic signal enhancement of up to 649% after 10 hours. We then used this system to treat an orthotopic model of colon cancer via luciferase-positive CT26 cells. Our data showed that tumor burden decreased by 44.7% ± 4.8% when treated with the PTX-MB @ PLGA NPs versus the empty PLGA carrier. This work presents a direct strategy to simultaneously monitor drug release biodistribution.
关键词： Photoacoustic imaging,Paclitaxel,Image-guided drug delivery,PLGA nanoparticles,Drug-release,Cancer therapy,in vivo monitoring
更新于2025-09-16 10:30:52
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In Vivo Reflection-Mode Photoacoustic Microscopy Enhanced by Plasmonic Sensing with an Acoustic Cavity
摘要： Relying on high-sensitivity refractive index sensing and a highly constrained evanescent field of surface plasmon resonance (SPR), broadband photoacoustic (PA) pressure transients were measured using an SPR sensor instead of routinely used piezoelectric ultrasonic transducers. An acoustic cavity made from stainless steel and having a designed ellipsoidal inner surface redirected laser-induced PA waves from the PA excitation spot to the SPR sensor. By incorporating the SPR sensor with the acoustic cavity, we developed optical-resolution photoacoustic microscopy (OR-PAM) with multiple advantages, including reflection-mode signal capture, improved PA detection sensitivity, increased PA spectral bandwidth as broad as ～98 MHz, and micrometer-scale lateral resolution. This allowed label-free volumetric PA imaging of vasculature in not only the thin ear but also the thick forelimb of living mice. With these combined advantages, our OR-PAM system potentially offers more opportunities for biomedical investigation, for example, when studying microcirculations in the eye and cortex.
关键词： reflection mode,high sensitivity,surface plasmon resonance,in vivo vascular imaging,photoacoustic imaging
更新于2025-09-12 10:27:22
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Preparation of silicon naphthalocyanine nanoparticles by laser ablation in liquid and their optical properties
摘要： Silicon naphthalocyanine nanoparticles were successfully prepared by laser ablation in liquid. Silicon 2,3-naphthalocyanine bis(trihexylsilyloxide) powders in deionized water were irradiated with nanosecond-pulsed laser (Nd:YAG, SHG) to prepare nanoparticles. The prepared nanoparticles were investigated by scanning electron microscopy, dynamic light scattering and spectrophotometry. The shape was polygonal and partially spherical. The primary and secondary particle sizes were reduced with an increase in laser fluence. Absorbance was increased at low laser fluence and was constant at high laser fluence with the increase in laser fluence. The ratio of the two absorbance peaks was changed with the increase in laser fluence.
关键词： optical properties,laser ablation in liquid,nanoparticles,photoacoustic imaging,silicon naphthalocyanine
更新于2025-09-12 10:27:22
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Organic semiconducting polymer amphiphile for near-infrared-II light-triggered phototheranostics
摘要： Development of near-infrared-II (NIR-II) light responsive nano-agents with high photothermal stability, high photothermal conversion efficiency (PCE), and excellent biocompatibility for photoacoustic (PA) imaging-guided photothermal therapy (PTT) is of tremendous significance. In spite of the superiority of organic semiconducting polymer nanoparticles (OSPNs) in PA imaging-guided PTT, the limited absorption in the first NIR (NIR-I) window and metastable nanostructure of OSPNs resulting from commonly used preparation methods based on nanoprecipitation or reprecipitation compromise their in vivo phototheranostic performance. Herein we design and synthesize a novel NIR-II absorbing organic semiconducting polymer amphiphile (OSPA) to enhance the structural stability of OSPNs. With prominent optical properties, low toxicity, and a suitable size, OSPA not only efficiently labels and kills cancer cells under NIR-II irradiation but also accumulates at the tumor of living mice upon intravenous injection, allowing efficient NIR-II light-triggered phototheranostics toward tumor. The developed OSPA has promising potential for fabricating multifunctional nanoplatforms to enable multimodal theranostics.
关键词： second near-infrared window,photoacoustic imaging,semiconducting polymer,amphiphile,photothermal therapy
更新于2025-09-12 10:27:22
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LED-based photoacoustic imaging for monitoring angiogenesis in fibrin scaffolds
摘要： Vascularization of engineered constructs is required to integrate an implant within the host blood supply. The ability to non‐invasively monitor neovascularization of an implanted construct is ultimately critical for translation. Laser speckle contrast analysis (LASCA), a widely used imaging technique within regenerative medicine, has high spatial resolution but offers limited imaging depth and is only sensitive to perfused blood vessels. As an emerging technology, photoacoustic (PA) imaging can provide centimeters of imaging depth and excellent sensitivity in vascular mapping. PA imaging in combination with conventional ultrasound (US) imaging offers a potential solution to this challenge in regenerative medicine. In this work, we used an LED‐based PA‐US dual system to image and monitor angiogenesis over 7 days in fibrin‐based scaffolds subcutaneously implanted in mice. Scaffolds, with or without basic fibroblast growth factor (bFGF), were imaged on day 0 (i.e., post implantation), 1, 3, and 7 with both LASCA and PA‐US imaging systems. Quantified perfusion measured by LASCA and PA imaging were compared with histologically‐determined blood vessel density on day 7. Vessel density corroborated with changes in perfusion measured by both LASCA and PA. Unlike LASCA, PA imaging enabled delineation of differences in neovascularization in the upper and the lower regions of the scaffold. Overall, this study has demonstrated that PA imaging could be a non‐invasive and highly sensitive method for monitoring vascularization at depth in regenerative applications.
关键词： Photoacoustic Imaging,LED-based Imaging,Fibrin Scaffolds,Tissue Engineering,Angiogenesis
更新于2025-09-11 14:15:04