研究目的
To develop an optical-resolution photoacoustic microscopy (OR-PAM) system that utilizes a surface plasmon resonance (SPR) sensor for high-sensitivity, broadband photoacoustic signal detection, enabling reflection-mode imaging of vasculature in both thin and thick biological tissues.
研究成果
The developed SPR-based OR-PAM system offers high-sensitivity, broadband PA signal detection with reflection-mode imaging capability, enabling detailed visualization of vasculature in both thin and thick biological tissues. This advancement opens up new possibilities for biomedical research, particularly in studying microcirculations in complex anatomical structures.
研究不足
The PA detection bandwidth was limited by inner surface imperfections of the acoustic cavity and high-frequency acoustic energy attenuation. The system's depth resolution is slightly worse than its lateral resolution due to the axial resolution being determined by the acoustic spectrum of the ultrasonic detector.
1:Experimental Design and Method Selection:
The study employed a Kretschmann-type SPR sensor for PA signal detection, integrating it with a customized acoustic cavity to enhance signal sensitivity and enable reflection-mode imaging.
2:Sample Selection and Data Sources:
In vivo imaging was performed on the ear and forelimb of living mice to demonstrate the system's capability.
3:List of Experimental Equipment and Materials:
A He-Ne laser for SPR interrogation, a Nd:YAG laser for PA excitation, a balanced photodiode for differential light detection, and a stainless-steel acoustic cavity with an ellipsoidal inner surface.
4:Experimental Procedures and Operational Workflow:
The system was configured for reflection-mode imaging, with PA signals redirected and focused by the acoustic cavity onto the SPR sensor. Raster scanning was implemented for volumetric imaging.
5:Data Analysis Methods:
PA signals were analyzed in both time and frequency domains to assess system performance, including sensitivity and bandwidth.
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Doublet lens
AC254-060-A
Thorlabs
Focusing the PA excitation light onto the sample
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He-Ne laser
HNL100L-EC
Thorlabs
Interrogation light source for SPR sensor
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Polarizer
LPVISE100-A
Thorlabs
Polarizing the interrogation light
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Half-wavelength plate
WPH10M-633
Thorlabs
Adjusting the polarization state of the interrogation light
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Balanced photodiode
PDB435a
Thorlabs
Differential light detection for SPR sensor
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Nd:YAG laser
SPOT-532
Elforlight
Excitation source of PA signals
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