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Structural, morphological and opticalproperties of spray-formed silver-doped zinc sulphide thin films
摘要： The study focused on the qualities of spray-formed Silver-doped Zinc sulphide (ZnS) thin films sprayed on soda-lime glass (slg) substrate. Silver-doped and undoped ZnS thin films with 0%, 1%, 3% and 5% Silver concentrations were deposited. The qualities of the synthesized films were investigated using x-ray diffractometry, scanning electron microscopy, Fourier transforms infrared (FTIR) spectrometry, UV-VIS spectrophotometry, Raman spectrometry and contact angle techniques. The x-ray diffraction data identified cubic structures for the thin films. Scanning electron microscopy shows the presence of agglomerates of nanoparticles and pores in the thin films. The thin films’ crystallite size ranges between 3.107 and 4.103 nm. FTIR revealed the chemical bonds in the film. The transmittance of the thin films is between 42.35 and 81.86% at 550 nm, the energy gap is observed within a range of 3.11 and 3.60 eV while the indices of refraction are in the range of 1.52 to 3.81 at 550 nm wavelength. Photoluminescence result shows Sulphur vacancies. A hydrophilic surface feature of the film was revealed by the contact angle measurement.
关键词： silver,Thin film,zinc sulphide,Raman spectrometry
更新于2025-11-14 15:16:37
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EXPRESS: Analysis of Natural Gas Using a Portable Hollow-Core Photonic-Crystal-Coupled Raman Spectrometer
摘要： The low accessibility of natural gas fields and transporting pipelines requires portable online analyzers of the composition of natural gas, ensuring nearly chromatographic precision and capable of in situ analysis of a wide range of gases, including infrared-inactive ones (hydrogen, oxygen, nitrogen, chlorine). We have developed an express method of gas analysis meeting all requirements for the analysis of natural gas and its derivative mixtures using a portable 532 nm Raman spectrometer rigidly connected to a hollow-core crystal photonic fiber.
关键词： hollow-core photonic crystal fiber,HC-PCF,Raman spectrometry,gas chromatography,natural gas,calibration gas mixture
更新于2025-09-23 15:21:01
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Convenient Construction of Orthogonal Dual Aptamer-Based Plasmonic Immunosandwich Assay for Probing Protein Disease Markers in Complex Samples and Living Animals
摘要： Aptamers, due to their outstanding merits including simple synthesis and easy modification, have been widely used as antibody alternatives to construct novel immunosandwich assays. Dual aptamer-based sandwich assays exhibit multiple advantages over conventional immunosandwich assays and single aptamer-based sandwich assays. However, their construction is hampered by the limited knowledge of binding orthogonality of aptamers reported in literature. Herein we present a new strategy for conveniently constructing an orthogonal dual aptamer-based plasmonic immunosandwich assay (odA-PISA) for probing proteins in complex samples and living animals. An orthogonal aptamer pair was first efficiently selected from aptamers reported in literature by affinity capillary electrophoresis (ACE). Then, a target protein-capturing gold thin layer-coated probe and silver nanoparticle-based Raman labeling nanotags were conveniently prepared with the selected aptamers and used to construct the assay. The double aptamers used ensured the specificity while the plasmonic coupling effect between the target-capturing probe and Raman nanotags generated significantly enhanced Raman signal intensity, providing high sensitivity. As a proof of principle, alkaline phosphatase (ALP) was used as the target. The constructed odA-PISA exhibited high specificity and high sensitivity toward ALP, giving cross-reactivity ≤ 4.2% and limit of detection (LOD) of 3.8 pM (S/N = 4). Quantitative determination of ALP in human serum and probing ALP in tumor-bearing mice were achieved, showing the great application potential of the method. This strategy is widely applicable to other protein disease markers. Therefore, it opened a new access to the construction of sensitive dual aptamer-based sandwich assays for real-world applications particularly disease diagnosis.
关键词： aptamer,binding orthogonality,Raman spectrometry,plasmonic immunosandwich assay,disease marker
更新于2025-09-23 15:19:57
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Detection of Metabolic Changes Induced via Drug Treatments in Live Cancer Cells and Tissue Using Raman Imaging Microscopy
摘要： Isocitrate dehydrogenase 1 (IDH1) mutations in gliomas, fibrosarcoma, and other cancers leads to a novel metabolite, D-2-hydroxyglutarate, which is proposed to cause tumorigenesis. The production of this metabolite also causes vulnerabilities in cellular metabolism, such as lowering NADPH levels. To exploit this vulnerability, we treated glioma and fibrosarcoma cells that harbor an IDH1 mutation with an inhibitor of nicotinamide adenine dinucleotide (NAD+) salvage pathway, FK866, and observed decreased viability in these cells. To understand the mechanism of action by which the inhibitor FK866 works, we used Raman imaging microscopy and identified that proteins and lipids are decreased upon treatment with the drug. Raman imaging showed a different distribution of lipids throughout the cell in the presence of the drug compared with the untreated cells. We employed nuclear magnetic resonance NMR spectroscopy and mass spectrometry to identify the classes of lipids altered. Our combined analyses point to a decrease in cell division due to loss of lipid content that contributes to membrane formation in the in vitro setting. However, the FK866 drug did not have the same potency in vivo. The use of Raman imaging microscopy indicated an opposite trend of lipid distribution in the tissue collected from treated versus untreated mice when compared with the cells. These results demonstrate the role of Raman imaging microscopy to identify and quantify metabolic changes in cancer cells and tissue.
关键词： NAD+ synthesis,tissue imaging,single cell imaging,microscopy,Raman spectrometry,fibrosarcoma IDH1
更新于2025-09-19 17:15:36
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A novel 3D printed negative pressure small sampling system for bubble-free liquid core waveguide enhanced Raman spectroscopy
摘要： Liquid core waveguide (LCW) is well-known as an effective fiber enhanced approach for Raman spectroscopy with features of long optical path and small sampling size. However, inevitable air bubbles introduced in the LCW tube possibly caused light scattering, refraction and reflection so as to further hamper the quantitative analysis. In this work, to eliminate air bubbles, a novel negative pressure system combined with 3D printing was first utilized for the enhanced Raman spectroscopy on the principle of the gas permeability of LCW tube. After optimization, the LCW tube made of Teflon-AF was inserted into a D-shaped support with an internal channel manufactured by 3D printing to create a sealed space; then, air pressure outside the LCW tube was reduced to create a negative pressure via diaphragm pump and magnetic valve controlled by computer. Under adjustable negative pressure, not only can liquid sample be introduced into the LCW tube automatically, but air bubbles can also be removed through the tube wall simply and completely. For real samples, the assembled apparatus was employed as the small sampling system of Raman spectrometer to measure rhodamine B and ethanol in solutions, with the highest 82-fold (ethanol) enhancement of analytical sensitivity vs. the traditional colorimetric ware. The limit of detections (LODs) were 0.7 μg/mL rhodamine B and 0.03% (v:v) ethanol with only 250 μL sample consumption; their linear correlation coefficients (r) were 0.998 and 0.999 in the range from 2 μg/mL to 25 μg/mL (rhodamine B) and 0.1%–5% (ethanol), respectively. It is worth mentioning that the intraday stability and 7-days reproducibility can be both controlled within 7%, which is extremely superior to the previous enhanced Raman spectroscopy. For another, 3D printing enables the LCW detection system more integrated and easier to assemble. So, the proposed method proves many advantages, such as stability, sensitivity, and quickness, in addition of effective physical enhancement, low sample consumption, and long light path. Considering the flexibility of LCW tube, as a versatile module, the negative pressure LCW system should be further suitable to ultraviolet, fluorescence and other detectors, which reveals a favorable application prospect for the fast testing instruments.
关键词： Bubble-free,Liquid core waveguide,3D printing,Micro sampling,Enhanced Raman spectrometry,Fast testing,Negative pressure
更新于2025-09-19 17:13:59
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Determination of Sulfite in Botanical Medicine Using Headspace Thin-Film Microextraction and Surface Enhanced Raman Spectrometry
摘要： A facile method using headspace thin-film microextraction (HS-TFME) coupled with surface enhanced Raman spectrometry (SERS) has been developed for the determination of sulfite in traditional Chinese herbal medicine. The extraction substrate was synthesized by depositing urchin-like ZnO micron particles on glass sheets using chemical liquid phase deposition. Under the optimal conditions, the intensity of the SERS signal at 630–640 cm?1 provided a good linear relationship with the concentration of sulfite from 25 to 400 mg/kg, and the linear correlation coefficient (R) was 0.996 with a detection limit of 6 mg/kg. The method was employed for the determination of sulfite in herbal medicines, and the results were confirmed by a traditional distillation-titration method. Therefore, this developed HS-TFME-SERS method may play an important role in the rapid, simple, and selective determination of sulfite residues in Chinese herbal medicine and become a potentially universal method for this analyte in various solid samples.
关键词： Headspace thin-film microextraction (HS-TFME),scanning electron microscopy (SEM),X-ray diffraction (XRD),surface enhanced Raman spectrometry (SERS),transmission electron microscopy (TEM)
更新于2025-09-10 09:29:36
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BCAbox Algorithm Expands Capabilities of Raman Microscope for Single Organelles Assessment
摘要： Raman microspectroscopy is a rapidly developing technique, which has an unparalleled potential for in situ proteomics, lipidomics, and metabolomics, due to its remarkable capability to analyze the molecular composition of live cells and single cellular organelles. However, the scope of Raman spectroscopy for bio-applications is limited by a lack of software tools for express-analysis of biomolecular composition based on Raman spectra. In this study, we have developed the first software toolbox for immediate analysis of intracellular Raman spectra using a powerful biomolecular component analysis (BCA) algorithm. Our software could be easily integrated with commercial Raman spectroscopy instrumentation, and serve for precise analysis of molecular content in major cellular organelles, including nucleoli, endoplasmic reticulum, Golgi apparatus, and mitochondria of either live or fixed cells. The proposed software may be applied in broad directions of cell science, and serve for further advancement and standardization of Raman spectroscopy.
关键词： single cell assay,biomolecular analysis,Raman spectrometry,microscopy
更新于2025-09-09 09:28:46