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Near-Infrared Light Triggered Sulfur Dioxide Gas Therapy of Cancer
摘要： The exploitation of gas therapy platforms holds great promise as a 'green' approach for selective cancer therapy, however, it is often associated with some challenges, such as uncontrolled or insufficient gas generation and unclear therapeutic mechanisms. In this work, a gas therapy approach based on near-infrared (NIR) light triggered sulfur dioxide (SO2) generation was developed, and the therapeutic mechanism as well as in vivo anti-tumor therapeutic efficacy was demonstrated. A SO2 prodrug-loaded rattle-structured upconversion@silica nanoparticles (RUCSNs) was constructed to enable high loading capacity without obvious leakage, and to convert NIR light into ultraviolet (UV) light so as to activate the prodrug for SO2 generation. In addition, SO2 prodrug-loaded RUCSNs showed high cell uptake, good biocompatibility, intracellular tracking ability, and high NIR light triggered cytotoxicity. Furthermore, the cytotoxic SO2 was found to induce cell apoptosis accompanied with the increase of intracellular reactive oxygen species (ROS) levels and the damage of nuclear DNA. Moreover, efficient inhibition of tumor growth was achieved, associated with significantly prolonged survival of mice. Such NIR light-triggered SO2 therapy may provide an effective strategy to stimulate further development of synergistic cancer therapy platforms.
关键词： upconversion nanoparticles (UCNPs),gas therapy,cancer therapy,near-infrared (NIR),sulfur dioxide (SO2)
更新于2025-09-23 15:22:29
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A fluorescence method of detecting sulfur dioxide derivatives
摘要： A fluorescence spectrometry method is proposed to detect SO3 2? and α, β-unsaturated compounds, exhibiting remarkable blue-shift the maximum emission wavelength (from 650 nm to 555 nm). Preliminary experiments are conducted and results show that the method exhibits high selectivity and sensitivity toward sulfite over other common anions. The fluorescence intensity ratio at 555 nm and 650 nm (I555/I650) has a good linearly with sulfite concentration ranging from 0.5 to 15 μM with a detection limit of 0.28 μM. These results provide theoretical fundamentals for sulfite detection in practical water samples.
关键词： sulfur dioxide derivatives,sulfite detection,fluorescence method,nucleophilic addition reaction
更新于2025-09-23 15:21:01
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Design and synthesis of a novel colorimetric fluorescent probe for selective detection of sulfur dioxide in SH-SY5Y neuroblastoma cells and its application in Traditional Chinese Medicines
摘要： Sulfur fumigation has attracted more and more attention as one of the important post-harvest processing methods for some Traditional Chinese Medicines (TCMs) in the last decade. However, sulfur-fumigated TCMs have recently emerged as a controversial topic due to their potential detrimental effect on the safety and efficacy, as some sulfur-fumigated TCMs contain lots of sulfur dioxide derivatives. Additionally, high levels of the sulfur dioxide derivatives could cause some diseases and dangerous environmental pollutant. In this work, a fast response time, low limit of detection and high fluorescence quantum yield probe DTCC was designed and synthesized to detect SO2 derivatives based on coumarin-thiophene dye which was fused with a coumarin moiety and 2-thiophenecarboxaldehyde. Probe DTCC exhibited fast response time (less than 10 s), satisfactory selectivity for SO2 derivatives in the presence of other ROS and excellent sensitivity for SO2 derivatives with low limit of detection (0.23 μM) and widely linear range (0～100 μM). Furthermore, probe DTCC was successfully applied in fluorescent imaging in SH-SY5Y neurobalstoma cells with excellent membrane permeability and stability. It was also employed for monitoring the total SO2 derivatives in several real TCMs sample. These results illustrated that probe DTCC has an excellent capability for monitoring SO2 derivatives in living cells and real TCMs sample.
关键词： Fast responsive,Fluorescent probe,Coumarin scaffold,Bioimaging,Sulfur dioxide,Traditional Chinese Medicines
更新于2025-09-19 17:15:36
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A new ratiometric fluorescence assay based on resonance energy transfer between biomass quantum dots and organic dye for the detection of sulfur dioxide derivatives
摘要： Sulfur dioxide (SO2) is considered as the fourth gas signal molecule after nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). It plays important roles in several physiological processes. Therefore, the design and synthesis of nanoprobes for the detection of SO2 derivatives in cells is of great significance. Herein, we report a new ratiometric fluorescence nanoprobe based on resonance energy transfer (RET) between biomass quantum dots (BQDs) and organic dye (DMI) for the detection of SO2 derivatives. The proposed ratiometric fluorescence assay allows the determination of HSO3? in the range of 1.0 to 225 μM with a detection limit of 0.5 μM. Importantly, the proposed ratiometric fluorescence nanoprobe exhibits a high photostability and good selectivity for HSO3? over other chemical species including H2S and biological mercaptans. Quantitation of HSO3? in cell lysates by using the nanoprobe is demonstrated.
关键词： ratiometric fluorescence,resonance energy transfer,organic dye,Sulfur dioxide,biomass quantum dots,nanoprobe
更新于2025-09-16 10:30:52
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Visualizing Endogenous Sulfur Dioxide Derivatives in Febrile Seizure-Induced Hippocampal Damage by a Two-Photon Energy Transfer Cassette
摘要： Febrile seizure (FS), a frequently encountered seizure disorder in pediatric populations, can cause hippocampus damage. It has been elucidated that sulfur dioxide (SO2) content is overproduced during the development of FS and related brain injury. Thus, in situ monitoring the level of endogenous SO2 in FS-related models is helpful to estimate the pathogenesis of FS-induced brain injury, but effect detection method remains to be explored. Herein, we developed a two-photon energy transfer cassette based on acedan-anthocyanidin scaffold, TP-Ratio-SO2, enabling to achieve this purpose. TP-Ratio-SO2 specifically responds to SO2 derivatives (HSO3-/SO32-) in ultrafast fashion (less than 3s), and HSO3-/SO32- can be sensitively determined with a detection limit of 26 nM. Moreover, it exhibits significant changes in two well-resolved ?uorescence emission (?λ = 140 nm) by reacting with HSO3-/SO32-, behaving as a ratiometric fluorescent sensor. Importantly, ratiometric imaging of endogenous SO2 derivatives generation in hyperpyretic U251 cells and as well as in rat model of FS-treated hippocampus damage were successfully carried out by TP-Ratio-SO2, demonstrating that it may be a promising tool for studying the role of SO2 in FS-associated neurological diseases.
关键词： Two-photon fluorescence probe,Febrile seizure,Sulfur dioxide,Ratiometric imaging,Hippocampus damage
更新于2025-09-09 09:28:46
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Sulfur Dioxide (SO2) Monitoring Over Kirkuk City Using Remote Sensing Data
摘要： Air pollution mapping is now being an important issue to manage and enhance the environment of a city. The major problems of air pollution mapping is the data acquisition due to the high cost of instruments and the high spatial distribution requirements. This study aimed to monitor Sulfur Dioxide over Kirkuk city using Landsat-8 thermal bands to provide Department of Environment Kirkuk with low-cost Sulfur Dioxide concentration maps to better manage the city. The study used correlation analysis to find a relationship between Sulfur Dioxide ground-based measurements and satellite data. The ground-based measurements were collected from (17) stations distributed in Kirkuk city in January, 2014 using NOVA device to measure SO2 concentrations. The research showed a good correlation between ground- based measurements and satellite data with (R2=0.48 for band 11 and R2= 0.52 for band 10). Therefore, the study resulted that with band 10 of Landsat-8 data, better SO2 can be monitored than using band 11. It is recommended to other researchers to investigate the ability of free remote sensing data to monitor all elements that specify the air quality of a city.
关键词： LST,Environment,Kirkuk city,SO2,TIRS,Remote sensing,GIS,Air pollution,Sulfur dioxide
更新于2025-09-04 15:30:14
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Sulfur dioxide (SO<sub>2</sub>) vertical column density measurements by Pandora spectrometer over the Canadian oil sands
摘要： Vertical column densities (VCDs) of SO2 retrieved by a Pandora spectral sun photometer at Fort McKay, Alberta, Canada, from 2013 to 2015 were analysed. The Fort McKay site is located in the Canadian oil sands region, approximately 20 km north of two major SO2 sources (upgraders), with total emission of about 45 kt yr?1. Elevated SO2 VCD values were frequently recorded by the instrument, with the highest values of about 9 Dobson Units (DU; DU = 2.69 × 1016 molecules cm?2). Comparisons with co-located in situ measurements demonstrated that there was a very good correlation between VCDs and surface concentrations in some cases, while in other cases, elevated VCDs did not correspond to high surface concentrations, suggesting the plume was above the ground. Elevated VCDs and surface concentrations were observed when the wind direction was from south to southeast, i.e. from the direction of the two local SO2 sources. The precision of the SO2 measurements, estimated from parallel measurements by two Pandora instruments at Toronto, is 0.17 DU. The total uncertainty of Pandora SO2 VCD, estimated using measurements when the wind direction was away from the sources, is less than 0.26 DU (1σ ). Comparisons with integrated SO2 pro?les from concurrent aircraft measurements support these estimates.
关键词： Pandora spectrometer,vertical column density,Sulfur dioxide,oil sands,air quality
更新于2025-09-04 15:30:14
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Polymeric Micelles Encapsulating a Small Molecule SO <sub/>2</sub> Fluorescent Probe Exhibiting Novel Analytical Performance and Enhanced Cellular Imaging Ability
摘要： Because of the limited knowledge on the relationship between molecular structure and analytical performance, developing a small molecule fluorescent probe with desirable response properties is usually a laborious work. On the other hand, the application of small molecule fluorescent probe in biological samples is always limited due to the unwanted interaction between dyes and biomacromolecules. Polymer micelles, thanks to its unique core?shell structure, may have the potential to improve these situations. However, utilization of polymer micelles to improve these situations is rarely explored. Herein, we engineered the first micellar SO2 nanoprobe Nano-Cz by self-assembly of a carbazole-based SO2 small molecule probe and an amphiphilic copolymer (DSPE-mPEG2000). The optical and cell imaging experiments revealed that Nano-Cz can work in 100% aqueous environment and act as an effective mitochondrial-targeting ratio SO2 nanoprobe. Compared with the single small molecule probe, Nano-Cz showed extraordinary large dynamic response range (0?0.7 mM vs 0?50 μM), eliminated signal interference from DNA and superior cellular imaging performance. These results clearly show the ability of polymer micelles in modulating sensors’ analytical performance and reducing the signal interference from the unwanted interaction between small molecule probe and biomacromolecule, indicating that polymer micelles encapsulating single small molecule probe can provide us an alternative strategy to explore sensors with various performance and promote the biological application of fluorescent sensors. In addition, we hope that more and more polymer micelles would be used to construct biosensors in the future.
关键词： sensing performance,biological application,sulfur dioxide,polymer micelles,fluorescent sensor
更新于2025-09-04 15:30:14