- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Shell-free Copper Indium Sulfide Quantum Dots Induce Toxicity <i>In Vitro</i> and <i>In Vivo</i>
摘要： Semiconductor quantum dots (QDs) are attractive fluorescent contrast agents for in vivo imaging due to their superior photophysical properties, but traditional QDs comprise toxic materials such as cadmium or lead. Copper indium sulfide (CuInS2, CIS) QDs have been posited as a non-toxic and potentially clinically translatable alternative; however, previous in vivo studies utilized particles with a passivating zinc sulfide (ZnS) shell, limiting direct evidence of the biocompatibility of the underlying CIS. For the first time, we assess the biodistribution and toxicity of unshelled CIS and partially zinc-alloyed CISZ QDs in a murine model. We show that bare CIS QDs breakdown quickly, inducing significant toxicity as seen in organ weight, blood chemistry, and histology. CISZ demonstrate significant, but lower, toxicity compared to bare CIS, while our measurements of core/shell CIS/ZnS are consistent with literature reports of general biocompatibility. In vitro cytotoxicity is dose-dependent on the amount of metal released due to particle degradation, linking degradation to toxicity. These results challenge the assumption that removing heavy metals necessarily reduces toxicity: indeed, we find comparable in vitro cytotoxicity between CIS and CdSe QDs, while CIS caused severe toxicity in vivo compared to CdSe. In addition to highlighting the complexity of nanotoxicity and the differences between the in vitro and in vivo outcomes, these unexpected results serve as a reminder of the importance of assessing the biocompatibility of core QDs absent the protective ZnS shell when making specific claims of compositional biocompatibility.
关键词： in vivo imaging,biodegradable,CIS,nanotoxicity,nanomedicine,CuInS2,fluorescent contrast agent,QDs
更新于2025-09-19 17:13:59
-
Nanocytotoxicity using matrix-assisted laser desorption ionization mass spectrometry
摘要： The analysis of bacteria species using MALDI-MS is simple and fast compared with traditional analysis methods such as cell culture, which requires days and sometime weeks.
关键词： nanocytotoxicity,matrix-assisted laser desorption ionization mass spectrometry,nanomedicine,antibacterials
更新于2025-09-19 17:13:59
-
[IEEE 2019 IEEE 4th International Conference on Integrated Circuits and Microsystems (ICICM) - Beijing, China (2019.10.25-2019.10.27)] 2019 IEEE 4th International Conference on Integrated Circuits and Microsystems (ICICM) - A Laser Modulation Driver in 130-nm CMOS Technology for Quantum Key Distribution System
摘要： The imaging of dysregulated cell-surface receptors (or biomarkers) is a potential means of identifying the presence of cancer with high sensitivity and specificity. However, due to heterogeneities in the expression of protein biomarkers in tumors, molecular imaging technologies should ideally be capable of visualizing a multiplexed panel of cancer biomarkers. Recently, surface-enhanced Raman-scattering (SERS) nanoparticles (NPs) have attracted wide interest due to their potential for sensitive and multiplexed biomarker detection. In this review, we focus on the most recent advances in tumor imaging using SERS-coded NPs. A brief introduction of the structure and optical properties of SERS NPs is provided, followed by a detailed discussion of key imaging issues such as the administration of NPs in tissue (topical versus systemic), the optical configuration and imaging approach of Raman imaging systems, spectral demultiplexing methods for quantifying NP concentrations, and the disambiguation of specific versus nonspecific sources of contrast through ratiometric imaging of targeted and untargeted (control) NP pairs. Finally, future challenges and directions are briefly outlined.
关键词： molecular imaging,fiberoptic probes,Biomarkers,cancer detection,nanomedicine,tumors,Raman spectroscopy,biomedical optical imaging
更新于2025-09-19 17:13:59
-
Radiology, Lasers, Nanoparticles and Prosthetics || 14. Nanoparticles for nanomedical applications
摘要： Nanomedicine is a multidisciplinary science and technology field that has emerged over the past 10–20 years. It involves medical physics, materials sciences, biochemistry, biomedicine, pharmaceutics, polymer sciences, clinical sciences, and possibly further fields. Nanomedicine is commonly defined as “The medical application of nanotechnology for diagnosis, treatment and the general management of human health”. As such, nanomedicine promises more sensitive diagnostics and more precise treatment of certain diseases, in particular of cancer.
关键词： Nanomedicine,Theranostic nanoparticles,Nanoparticles,Therapeutics,Diagnostics
更新于2025-09-16 10:30:52
-
Laser-Ablative Synthesis of Isotope-Enriched Samarium Oxide Nanoparticles for Nuclear Nanomedicine
摘要： Nuclear nanomedicine is an emerging field, which utilizes nanoformulations of nuclear agents to increase their local concentration at targeted sites for a more effective nuclear therapy at a considerably reduced radiation dosage. This field needs the development of methods for controlled fabrication of nuclear agents carrying nanoparticles with low polydispersity and with high colloidal stability in aqueous dispersions. In this paper, we apply methods of femtosecond (fs) laser ablation in deionized water to fabricate stable aqueous dispersion of 152Sm-enriched samarium oxide nanoparticles (NPs), which can capture neutrons to become 153Sm beta-emitters for nuclear therapy. We show that direct ablation of a 152Sm-enriched samarium oxide target leads to widely size- and shape-dispersed populations of NPs with low colloidal stability. However, by applying a second fs laser fragmentation step to the dispersion of initially formed colloids, we achieve full homogenization of NPs size characteristics, while keeping the same composition. We also demonstrate the possibility for wide-range tuning of the mean size of Sm-based NPs by varying laser energy during the ablation or fragmentation step. The final product presents dispersed solutions of samarium oxide NPs with relatively narrow size distribution, having spherical shape, a controlled mean size between 7 and 70 nm and high colloidal stability. The formed NPs can also be of importance for catalytic and biomedical applications.
关键词： nuclear nanomedicine,samarium (Sm) oxide nanoparticles,pulsed laser ablation in liquids,femtosecond laser ablation and fragmentation
更新于2025-09-16 10:30:52
-
Graphene Quantum Dots and Their Applications in Bioimaging, Biosensing, and Therapy
摘要： Graphene quantum dots (GQDs) are carbon-based, nanoscale particles that exhibit excellent chemical, physical, and biological properties that allow them to excel in a wide range of applications in nanomedicine. The unique electronic structure of GQDs confers functional attributes onto these nanomaterials such as strong and tunable photoluminescence for use in fluorescence bioimaging and biosensing, a high loading capacity of aromatic compounds for small-molecule drug delivery, and the ability to absorb incident radiation for use in the cancer-killing techniques of photothermal and photodynamic therapy. Recent advances in the development of GQDs as novel, multifunctional biomaterials are presented with a focus on their physicochemical, electronic, magnetic, and biological properties, along with a discussion of technical progress in the synthesis of GQDs. Progress toward the application of GQDs in bioimaging, biosensing, and therapy is reviewed, along with a discussion of the current limitations and future directions of this exciting material.
关键词： biosensing,quantum dots,drug delivery,nanomedicine,bioimaging,graphene
更新于2025-09-12 10:27:22
-
Dual-functional supramolecular nanohybrids of quantum dot/biopolymer/chemotherapeutic drug for bioimaging and killing brain cancer cells in vitro
摘要： Glioblastoma (GBM) is the utmost aggressive and lethal primary brain cancer, which has a poor prognosis and remains virtually incurable. Nanomedicine with emerging disruptive nanotechnology alternatives, including designed supramolecular nanohybrids has excellent potential as multimodal tools against cancer by combining nanomaterials, biomacromolecules, and drugs. Thus, we developed and constructed for the first time quantum dot-biopolymer-drug nanohybrids based on host-guest chemistry for simultaneous bioimaging, targeting, and anti-cancer drug delivery against GBM cells in vitro. ZnS fluorescent quantum dots (ZnS-QDs) were produced using chemically modified polysaccharide, carboxymethylcellulose (CMC), as water-soluble capping ligand and biofunctional layer via a facile one-step eco-friendly aqueous colloidal process at room temperature and physiological pH. These hybrid inorganic-organic nanocolloids (ZnS@CMC) were electrostatically conjugated with doxorubicin (DOX) anti-cancer drug forming innovative supramolecular complexes (ZnS@CMC-DOX) for amalgamating bioimaging and killing cancer cells. These nanoconjugates were characterized regarding their optical and physicochemical properties combined with morphological and structural features. The cytocompatibility was evaluated by MTT assay using healthy and GBM cells. The results showed that ultra-small ZnS-QDs were expertly produced uniform nanocolloids (average size = 3.6 nm). They demonstrated photoluminescence emission within the visible range of spectra. The cell viability results in vitro showed no cytotoxicity of ZnS@CMC nanohybrids towards both cell types. In summary, the novelty of this research relies on using a nanotheranostic strategy for developing ZnS@CMC-DOX nanohybrids with supramolecular vesicle-like structures. They behaved simultaneously as active fluorescent nanoprobes and nanocarriers with modulated drug release for bioimaging and killing malignant glioma cells proving the high potential for applications in cancer nanomedicine.
关键词： Nanocarriers,Cancer nanotheranostic,Nanocolloids,Nanoconjugates,Fluorescent nanoparticles,Nanomedicine,Nanohybrids,Nanoparticles
更新于2025-09-12 10:27:22
-
Quantifying the photothermal conversion efficiency of plasmonic nanoparticles by means of terahertz radiation
摘要： The accurate determination of the photothermal response of nanomaterials represents an essential aspect in many fields, such as nanomedicine. Specifically, photothermal cancer therapies rely on the precise knowledge of the light-to-heat transfer properties of plasmonic nanoparticles to achieve the desired temperature-induced effects in biological tissues. In this work, we present a novel method for the quantification of the photothermal effect exhibited by nanoparticles in aqueous dispersions. By combining the spatial and temporal thermal dynamics acquired at terahertz frequencies, the photothermal conversion efficiency associated with the geometry of the plasmonic nanoparticles can be retrieved in a noncontact and noninvasive manner. The proposed technique can be extended to the characterization of all those nanomaterials which feature a temperature-dependent variation of the refractive index in the terahertz regime.
关键词： terahertz radiation,plasmonic nanoparticles,photothermal conversion efficiency,nanomedicine,photothermal therapy
更新于2025-09-12 10:27:22
-
Controllable “Clicked-to-Assembled” Plasmonic Core–Satellite Nanostructures and Its Surface-Enhanced Fluorescence in Living Cells
摘要： The assembly of noble-metal core?satellite (CS) nanostructures is an appealing means to control their plasmonic properties for applications such as surface-enhanced ?uorescence or Raman scattering. However, till now there is a lack of some rapid or convenient methods to construct stable CS nanostructures. Here, we proposed a “clicked-to-assembly” strategy based on the fast and speci?c “click chemistry” reaction between trans-cyclooctene (TCO) and 1,2,4,5-tetrazine (Tz). The CS nanostructures were constructed within 8 min by simple mixing of TCO- or Tz-modi?ed nanoparticles (TCO-NPs or Tz-NPs) without any catalysts or heating required. Transmission electron microscopy experiments show that the constructed CS nanostructures are uniform, and particularly the number of “satellite” nanoparticles in the core surface is controllable by simply adjusting the feeding ratio of TCO-NPs or Tz-NPs in the reaction. The strong surface plasmon coupling e?ect (SPCE) was observed in these CS nanostructures, which was dependent on the coverage degree, size and composition of the satellite, and core NPs. The nanostructures with tuned surface plasmon resonance (SPR) e?ect were tried for the surface-enhanced ?uorescence in living cells. Such well-de?ned CS nanostructures could potentially serve as e?cient SPR-enhanced ?uorescent probes as diagnostics or biomedical imaging agents in nanomedicine.
关键词： core?satellite nanostructures,surface-enhanced ?uorescence,plasmonic properties,nanomedicine,click chemistry
更新于2025-09-11 14:15:04
-
Boron Nitride Nanotubes in Nanomedicine || Optical properties of boron nitride nanotubes: potential exploitation in nanomedicine
摘要： Nanotechnology is the ability and the art of exploiting nanometer-scale control on the size and the shape of a system, in order to obtain novel physicochemical properties. Chemical reactivity, optical response, and interactions with cells and tissues of nanotailored materials can be remarkably different from those of the bulk counterpart. The application of this flexibility and of the enormous potential that it brings along to the treatment, prevention, or diagnosis of diseases—that is, nanomedicine—is a novel opportunity that in spite of its young age is already representing a market valued at almost 80 billion USD in 2012 [1], with hundreds of nanomedicines and nanoformulations which are either approved or under clinical evaluation [2]. In the class of nanomaterials, boron nitride nanotubes (BNNTs) possess unique characteristics that make them particularly appealing. BNNTs are structural equivalents of carbon nanotubes (CNTs) with boron and nitrogen atoms replacing the carbon atoms of CNTs, and can be thought as a rolled sheet of hexagonal BN (h-BN) as depicted in Fig. 9.1a, b, and d. Despite their structural similarity with CNTs, they are comparable or superior to the latter in terms of several technologically relevant properties such as a thermal conductivity (at least in isotopically pure tubes) [3], thermal [4] and chemical [5] stability, piezoelectricity [6], and mechanical strength [7]. These peculiar advantages stem from the significant ionic component of the covalent bonds between B and N atoms.
关键词： Boron Nitride Nanotubes,Optical Properties,Nanotechnology,Nanomedicine
更新于2025-09-11 14:15:04