- 标题
- 摘要
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- 实验方案
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Ratiometric fluorescent probe based on pyrrole-modified rhodamine 6G hydrazone for the imaging of Cu2+ in lysosomes
摘要： A novel rhodamine-based Schiff base derivative was obtained via the simple condensation of substituted formyl-1H-pyrrole and rhodamine 6G hydrazone. Fluorescence resonance energy transfer enabled the subsequent use of the derivative as a naked-eye colorimetric and ratiometric fluorescent sensor for Cu2+ in semi-aqueous solution, and the existence of the morpholine group enabled the further application of the sensor in imaging Cu2+ in the lysosomes of HeLa cells.
关键词： ratiometric fluorescent probe,lysosome-targeting,rhodamine hydrazone,Cu2+,FRET,pyrrole
更新于2025-09-23 15:23:52
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A novel “turn-on” mitochondria-targeting near-infrared fluorescent probe for determination and bioimaging cellular hydrogen sulfide
摘要： Hydrogen sulfide (H2S) has been regarded as an important gas transmitter playing vital role in cytoprotective processes and redox signaling. It is very meaningful to monitor and analyze it in biosystem for obtaining important physiological and pathological information. Despite numerous fluorescent probes for cellular H2S have been reported in past decades, only a few have capability to detect mitochondrial H2S with near-infrared (NIR) emission. Therefore, a new mitochondria-targeting NIR fluorescent probe (Mito-NSH) for detection of cellular H2S was developed by introducing 2,4-dinitrophenyl ether into a novel dye (Mito-NOH). A large "turn-on" NIR fluorescence response was obtained due to thiolysis of ether to hydroxyl group when Mito-NSH was treated with NaHS. Moreover, Mito-NSH could quantitatively detect H2S at concentration ranging from 0 to 30 μM with a detection limit of 68.2 nM, and it exerts some superior optical properties, such as large stokes shift (107 nm), highly selectively mitochondria location, fast response and high selectivity to H2S. More impressively, it was successfully applied to imaging exogenous and endogenously generated H2S in living HeLa cells via confocal fluorescence microscopy.
关键词： Fast response,NIR fluorescent probe,"Turn-on" response,Hydrogen sulfide,Mitochondria-targeting
更新于2025-09-23 15:23:52
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An AIEgen-based fluorescent probe for highly selective and specific imaging of lipid droplets in L02 and HepG2 cells
摘要： Lipid droplets (LDs), as dynamic organelles, are associated with physiologically and pathologically metabolic processes. Especially, in human liver cancer cells (HepG2 cells) the lipid metabolism shows significant difference by comparing with the human normal liver cell (L02 cells). In this work, a new highly selective and specific fluorescent probe TPA-SD for the detection of LDs has been rationally designed and easily prepared from triphenylamine and salicylaldehyde, which possesses aggregation-induced emission (AIE) and excited state intramolecular proton transfer characteristics (ESIPT). Ideal probes for LDs imaging require high concentration accumulation of fluorophores in LDs and lack of self-quenching in the aggregated state, so the AIE property is necessary. TPA-SD can successfully and specifically accumulate in LDs, thus facilitating clearly distinguishing L02 cells from HepG2 cells according to obvious difference in the number of dot-shaped LDs marked by TPA-SD. The dot-shaped LDs in the HepG2 cells is found to be obviously more than that in the L02 cells. Moreover, the spatial distribution of LDs could be identified by TPA-SD. The results indicate TPA-SD possesses great potential in imaging and diagnosis of LDs-related diseases.
关键词： fluorescent probe,aggregation-induced emission,dot-shaped LDs number,lipid droplets (LDs) imaging
更新于2025-09-23 15:23:52
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Sensitive detection and imaging of endogenous peroxynitrite using a benzo[d]thiazole derived cyanine probe
摘要： Peroxynitrite is a short-lived endogenous reactive species and plays important roles in many physiological and pathological processes. In this work, we synthesized a near-infrared probe based on the structure of benzothiazole derived cyanine for determination of peroxynitrite (ONOO-). The designed probe specifically reacted with ONOO- through oxidative cleavage of conjugated C?C double bonds and generating the non-fluorescent product. Meanwhile, the characteristic absorption of the probe at 630 nm greatly decreased after reaction with ONOO-, accompanied by drastic color change from bright blue to green yellow, which exhibited a distinct visual feature. It was demonstrated that the probe could be used to measure ONOO- in a dose-response manner and had a detection limit lower as 26 nM. Furthermore, the probe Cy-SN was applied for the imaging of endogenous ONOO- in living cells by confocal microscopy, which showed good cell permeability and low cytotoxicity. Successful application of probe for exogenous colorimetric detection and endogenous fluorescence imaging of ONOO- is suggesting its great potential applications in biological analysis.
关键词： Colorimetric,Peroxynitrite,Benzothiazole derived cyanine,Fluorescent probe,Endogenous fluorescence imaging
更新于2025-09-23 15:23:52
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Boron doped carbon dots as a multifunctional fluorescent probe for sorbate and vitamin B12
摘要： Boron doped carbon dots (B-CD) were synthesized by a one-step hydrothermal method using phenylboronic acid as the starting material. They have an average size of about 3.3 nm, with excitation/emission wavelength of 247/323 nm and a quantum yield of 12%. The B-CD is shown to be viable fluorescent probe for sorbate (PS) and vitamin B12 (VB12). The fluorescence (FL) of the B-CD is quenched in the presence of PS or VB12 mainly coming from inner filter effect (IFE), but F?rster resonance energy transfer (FRET) from the B-CD (as a donor) to PS/VB12 (as an acceptor) cannot be excluded. The probe enables PS to be detected by fluorometry with a linear response in the 0.20–24 μM concentration range and a 6.1 nM detection limit (at 3σ/slope). For VB12, the data are 0.20–30 μM and 8.0 nM.
关键词： Fluorescent probe,Sorbic acid,Fluorescence quenching ratios,Intra-day precision,Selectivity,Inter-day precision,Quenching mechanism,Real sample analysis
更新于2025-09-23 15:23:52
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Disentangling topographic contributions to near-field scanning microwave microscopy images
摘要： We develop empirical models to predict the contribution of topographic variations in a sample to near-field scanning probe microwave microscopy (NSMM) images. In particular, we focus on |S11| images of a thin Perovskite photovoltaic material and a GaN nanowire. The difference between the measured NSMM image and this prediction is our estimate of the contribution of material property variations to the measured image. Prediction model parameters are determined from either a reference sample that is nearly free of material property variations or directly from the sample of interest. The parameters of the prediction model are determined by robust linear regression so as to minimize the effect of material property variations on results. For the case where the parameters are determined from the reference sample, the prediction is adjusted to account for instrument drift effects. Our statistical approach is fully empirical and thus complementary to current approaches based on physical models that are often overly simplistic.
关键词： Near-field scanning probe microwave microscopy,Signal extraction,GaN nanowire,Statistical methods,Perovskite materials,Atomic force microscopy
更新于2025-09-23 15:23:52
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Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push–Pull Fluorophore
摘要： Lipid droplets (LDs) are organelles composed of a lipid core surrounded by a phospholipid monolayer. Lately, LDs attracted a considerable attention due to recent studies demonstrating their role in a variety of physiological processes as well as diseases. Herein we synthesized a push-pull molecule named DAF (Dimethyl Aniline Furaldehyde) that possesses a strong positive solvatochromism in emission of 119 nm from toluene to methanol. Its impressive fluorogenic properties from water to oil (2000-fold) as well as its high quantum yields (up to 0.97) led us to investigate its ability to sense the distribution of polarity in live cells by fluorescence ratiometric imaging. When added to live cells and excited at 405 nm, DAF immediately and brightly stain lipid droplets using a blue channel (410-500 nm) and cytoplasm in a red channel (500-600 nm). DAF also proved to be compatible with fixation thus allowing 3D imaging of LDs in their cytoplasm environment. Taking advantage of DAF emission in two distinct channels, ratiometric imaging was successfully performed and led to the polarity mapping of the cell unraveling some heterogeneity in polarity within LDs of the same cell.
关键词： Environment Sensitive Fluorescent Probe,Lipid Droplets,Ratiometric Imaging,Multicolor Fluorescent imaging
更新于2025-09-23 15:23:52
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Enhancement strategies of targetability, response and photostability for in vivo bioimaging
摘要： Analyses of the physiology and pathology of active biochemical species in their native contexts are critical for early diagnosis and therapy. Optical imaging has emerged as one of the promising modalities for noninvasive and real-time visualization of important biomolecules or biological events, and it has witnessed major advances in the field of imaging in vitro and in vivo. In this review, we present a survey of common approaches and tactics for enhanced targetability, response rate, and photostability in bioimaging applications. Recently developed and representative examples are illustrated on the cellular and tissue levels.
关键词： targetability,bioimaging,photostability,response rate,fluorescent probe
更新于2025-09-23 15:23:52
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Construction of a Novel Fluorescent Probe for On-site Measuring Hydrogen Sulfide Levels in Food Samples
摘要： Hydrogen sulfide (H2S) has been involved in many foodstuffs and beverages. On-site measuring the H2S level in food samples was beneficial to the concerns about food safety and food quality. Herein, we rationally constructed a novel fluorescent probe to track the H2S levels in various food samples. The optical experiments indicated that the probe displayed highly selective and sensitive response to H2S. In addition, the probe can function well in a wide pH value condition, providing a reliable platform to sense H2S. The practical application demonstrated that the probe was applied for tracking H2S levels in banana, grape, and vinegar for the first time. Importantly, by fabricating test strips, the probe was first served to monitor the released H2S in raw meat without relying on elaborate instrumentation. Thus, the probe could be employed as a simple tool to measure the H2S levels in various food samples, as well as to monitor the fresh-like quality of raw meat under on-site conditions.
关键词： Fluorescent probe,On-site measurement,Hydrogen sulfide,Food sample,Test strip
更新于2025-09-23 15:23:52
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Caesium-Incorporated Triple Cation Perovskites Deliver Fully Reversible and Stable Nanoscale Voltage Response
摘要： Perovskite solar cells that incorporate small concentrations of Cs in their A-site have shown increased lifetime and improved device performance. Yet, the development of fully stable devices operating near the theoretical limit requires understanding how Cs influences perovskites’ electrical properties at the nanoscale. Here, we determine how the chemical composition of three perovskites (MAPbBr3, MAPbI3, and Cs-mixed) affects their short- and long-term voltage stabilities, with <50 nm spatial resolution. We map an anomalous irreversible electrical signature on MAPbBr3 at the mesoscale, resulting in local Voc variations of ~400 mV, and in entire grains with negative contribution to the Voc. These measurements prove the necessity of high spatial resolution mapping to elucidate the fundamental limitations of this emerging material. Conversely, we capture the fully reversible voltage response of Cs-mixed perovskites, containing small amounts of Cs, FA, and Br, demonstrating that the desired electrical output persists even at the nanoscale. The Cs-mixed material presents no spatial variation in Voc, as ion motion is restricted. Our results show that the nanoscale electrical behavior of the perovskites is intimately connected to their chemical composition and macroscopic response.
关键词： ion motion,MAPbI3,Cs-mixed perovskite,MAPbBr3,nanoscale voltage,perovskite solar cells,Kelvin probe force microscopy
更新于2025-09-23 15:23:52