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Design of Refolding DNA Aptamer on Single-Walled Carbon Nanotubes for Enhanced Optical Detection of Target Proteins
摘要： DNA aptamer conjugated single-walled carbon nanotube (Aptamer-SWNT) hybrids have demonstrated effective optical biosensors because of its high selectivity and specificity to a target protein, however, the understanding of SWNT hybridization with an aptamer forming a secondary or tertiary structure is still lacking. We study wrapping methods dependent optical biosensing modulation by insulin and platelet-derived growth factor (PDGF) using the Aptamer-SWNT hybrids and the Aptamer-Anchor-SWNT hybrids with a periodically sequenced single-stranded DNA (ssDNA) as anchoring phases. We observe that the refolding nature of the aptamer and its combination with an anchoring phase are critical to the hybridization, where the remarkable optical sensing properties are attributed to the wrapping procedures including the direct wrapping with sonication and the indirect wrapping through dialysis. The tetrameric parallel-stranded G-quadruplex conformation of insulin binding aptamers (IBA) shows an enhanced fluorescence response quenching when using the directly wrapped Aptamer-Anchor-SWNT hybrids. In addition, helix-structural refolding of PDGF binding aptamers (PBA) on the SWNT vicinity under the indirect wrapping exhibits anchoring length-dependent optical modulation. Furthermore, the consecutive centrifuging processes with the indirect wrapping demonstrate fluorescence response brightening, in which the diameter dependent brightening effect is observed by aptamer-protein interactions. This study provides an understanding the underlying conjugation nature of both the Aptamer-SWNT and the Aptamer-Anchor-SWNT hybrids formation, facilitating exceptional optical sensing modules with consideration of refolding feature of aptamers, selection of anchoring phases, wrapping methods and centrifuging process, and the hybridization voyage for DNA-SWNT platforms maneuvers their outcoming optical biosensing capabilities.
关键词： insulin,single-walled carbon nanotubes,PDGF,wrapping methods,optical biosensing,DNA aptamer
更新于2025-09-11 14:15:04
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Self-assembly of single-walled carbon nanotubes arrays with different line width
摘要： Single-walled carbon nanotubes (SWNTs) are one of the most promising nanoelectronic materials, However, there are still some application challenges, including purification, regular arrangement, interconnection, and so on. There is evidence that the array assembly can improve the performance of SWNTs. In this work, we develop a line-width controllable large-scale assembly of SWNTs based on aqueous solution, using electron beam (E-beam) lithography to construct self-assembled templates. The results show that SWNTs arrays of different line-width are obtained, which is possible to design the structure of SWNTs assembly to meet different functions.
关键词： Single-walled carbon nanotubes (SWNTs),self-assembly,line-width,electron beam (E-beam) lithography
更新于2025-09-11 14:15:04
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Enhanced Charge Carrier Transport in 2D Perovskites by Incorporating Single-Walled Carbon Nanotubes or Graphene
摘要： Two-dimensional (2D) organic-inorganic (hybrid) perovskites are considered promising candidates to replace conventional three-dimensional (3D) perovskites for solar cell applications as they have good resistance against moisture and UV light. However, the use of 2D perovskite is associated with a significant decrease in power efficiency resulting from their low photogenerated charge carrier density and poor charge transport. To improve power efficiency in 2D perovskites, highly crystalline films (near-single-crystal quality) of 2D perovskite needs to be synthesized where the alignment of the inorganic perovskite components is controlled to have vertical alignment with respect to the contacts to improve charge transport. In this work, we explored strategies to overcome this limitation, by integrating 2D perovskite with single-walled carbon nanotubes or graphene to enable more efficient extraction of charge carriers toward electric contacts. Longer carrier lifetimes were achieved after the incorporation of the carbon nanostructures in the films and at cell level, power efficiency increased by two-fold.
关键词： 2D perovskites,charge-carrier transport,solar cells,single-walled carbon nanotubes,graphene
更新于2025-09-11 14:15:04
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Chemometric Approaches for Developing Infrared Nanosensors to Image Anthracyclines
摘要： Generation, identification, and validation of optical probes to image molecular targets in a biological milieu remains a challenge. Synthetic molecular recognition approaches leveraging the intrinsic near-infrared fluorescence of single-walled carbon nanotubes is a promising approach for long-term biochemical imaging in tissues. However, generation of nanosensors for selective imaging of molecular targets requires a heuristic approach. Here, we present a chemometric platform for rapidly screening libraries of candidate single-walled carbon nanotube nanosensors against biochemical analytes to quantify fluorescence response to small molecules including vitamins, neurotransmitters, and chemotherapeutics. We further show this approach can be leveraged to identify biochemical analytes that selectively modulate the intrinsic near-infrared fluorescence of candidate nanosensors. Chemometric analysis thus enables identification of nanosensor-analyte ‘hits’ and also nanosensor fluorescence signaling modalities such as wavelength-shifts that are optimal for translation to biological imaging. Through this approach, we identify and characterize a nanosensor for the chemotherapeutic anthracycline doxorubicin, which provides an up to 17 nm fluorescence red-shift and exhibits an 8 μM limit of detection, compatible with peak circulatory concentrations of doxorubicin common in therapeutic administration. We demonstrate selectivity of this nanosensor over dacarbazine, a chemotherapeutic commonly co-injected with DOX. Lastly, we demonstrate nanosensor tissue compatibility for imaging of doxorubicin in muscle tissue by incorporating nanosensors into the mouse hindlimb and measuring nanosensor response to exogenous DOX administration. Our results motivate chemometric approaches to nanosensor discovery for chronic imaging of drug partitioning into tissues and towards real-time monitoring of drug accumulation.
关键词： fluorescence response,single-walled carbon nanotubes,infrared nanosensors,biological imaging,doxorubicin,chemometric approaches,anthracyclines
更新于2025-09-09 09:28:46
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Bipolar Thermoelectric Effects in Semiconducting Carbon Nanotubes: Description in Terms of One-Dimensional Dirac Electrons
摘要： The thermoelectric effects in semiconducting single-walled carbon nanotubes (SWCNTs) are investigated based on the linear response theory combined with the thermal Green’s function method. It is shown that the electronic states near the lowest conduction band minimum and the highest valence band maximum can be effectively described in terms of one-dimensional (1D) Dirac electrons to which a theoretical scheme is developed to describe the thermoelectric responses making it possible to study the effects of inter-band impurity scattering and in-gap states. Using the proposed scheme, the bipolar thermoelectric effects (i.e., the sign inversion of the Seebeck coefficient) in semiconducting SWCNTs observed in recent experiments are explained. Moreover, the temperature dependence of the Seebeck coefficient of semiconducting SWCNTs at low temperature is clarified.
关键词： thermal Green’s function method,Seebeck coefficient,linear response theory,Dirac electrons,semiconducting single-walled carbon nanotubes,thermoelectric effects
更新于2025-09-04 15:30:14