研究目的
采用半胱胺氧化的新方法来量化市售隐形眼镜及载有维生素E的隐形眼镜的氧气透过率。
研究成果
所开发的方法能可靠测量隐形眼镜的氧气透过率，多数镜片的测量结果与商业数值偏差不超过10%。20%的维生素E负载量对氧气透过率无显著影响。通过进一步设计改进可提升准确性与可靠性，使该方法适用于量化各类材料的氧气传输性能。
研究不足
潜在的误差来源包括测量过程中的空气进入（尤其是在滴眼液法中）、比色皿法中缺乏受控混合、镜片厚度和暴露面积的差异，以及可能的泄漏或残留氧气。建议的改进措施包括更好的密封、使用隔膜进行采样、添加混合系统以及设计专用比色皿。
1：实验设计与方法选择：
采用3D打印部件设计了两套定制系统——滴眼液瓶法和比色皿法。两套系统均将隐形眼镜作为半胱胺溶液氧气进入的唯一通道，通过紫外-可见分光光度法测量氧化速率，基于化学计量关系计算氧气透过率。
2：样本选择与数据来源：
使用市售隐形眼镜（如安视优舒视氧、安视优欧舒适、安视优天天抛、库博光学艾维瑞等）。半胱胺溶液配制于充氮除氧的磷酸盐缓冲盐水(PBS)中。
3：实验设备与材料清单：
设备包括创想三维CR-10 3D打印机、Monoprice Select 3D打印笔、热电Genysys 10S紫外-可见分光光度计、打孔器、石英比色皿、滴眼液瓶、铝箔、强力胶及多种化学试剂（半胱胺、乙醇、PBS、维生素E）。材料包含PLA线材及特定品牌隐形眼镜。
4：实验流程与操作步骤：
滴眼液瓶法通过在瓶身打孔，用3D打印盖固定隐形眼镜后注入半胱胺溶液，滴落液用于测量；比色皿法采用改良比色皿盖固定隐形眼镜，直接盛装半胱胺溶液随时间测定吸光度。两种方法均预先通氮除氧。
5：数据分析方法：
根据紫外-可见吸收数据测量半胱胺氧化速率，利用氧化速率与镜片氧气通量的化学计量方程计算氧气透过率。
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3D printer
CR-10
Creality
Used to create custom 3D-printed components for the experimental setups, such as caps and lids for eye drop bottles and cuvettes.
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3D printing pen
Select
Monoprice
Used for additional 3D printing, such as adding seals to cuvette lids.
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UV-Vis spectrophotometer
Genysys 10S
Thermospectronic
Used to measure the absorbance of cysteamine solutions to determine oxidation rates and calculate oxygen transmissibility.
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Contact lens
Acuvue Moist (Etafilcon A)
Johnson and Johnson Vision Care
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Acuvue TruEye (Narafilcon A)
Johnson and Johnson Vision Care
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Acuvue Oasys (Senofilcon A)
Johnson and Johnson Vision Care
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Dailies Aqua Comfort Plus (Nelfilcon A)
Alcon
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Dailies Total 1 (Delefilcon A)
Alcon
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Avaira (Enfilcon A)
CooperVision
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Eye drop bottle
United States Plastic Corp
Modified to hold the contact lens and cysteamine solution for the eye drop method of measuring oxygen transmissibility.
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Quartz cuvette
Used in the cuvette method to hold the cysteamine solution and allow direct measurement of absorbance through the contact lens.
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