1：Experimental Design and Method Selection:

The SPRi system was designed using a diode-pumped all-solid-state laser and galvanometer for fast angle scanning. A 4F system ensured the incident light excited the same area on the sensor chip regardless of the incident angle change.

2：Sample Selection and Data Sources:

The sensor unit included an equilateral prism made of BK7 glass and SPR sensor chips coated by a 48-nm-thick gold film. A PDMS flow cell was used to form a sealed flow cell.

3：List of Experimental Equipment and Materials:

Equipment included a 671-nm diode-pumped all-solid-state laser, galvanometer scanner, CMOS camera, and diffusers for speckle elimination.

4：Experimental Procedures and Operational Workflow:

The galvanometer was used for bidirectional scanning of the incidence angle. Speckle was eliminated using cascaded diffusers. The CMOS camera recorded intensity images at different incident angles.

5：Data Analysis Methods:

The diagonal spectrum curve-fitting algorithm and cubic spline interpolation algorithm were used for data analysis to detect the resonant angle of each pixel.