摘要： Background: Improvements in skin erythema and elasticity have been seen with topical application of platelet-rich plasma after fractional laser treatment. Nanofat, when injected in small needles into the dermis has shown improvements in tissue thickness, discoloration and wrinkle depth. Objective: To evaluate improvements in skin following a nonablative fractional laser (FXD) treatment combined with the application of a novel topical nanofat biocrème, called neo-U. Methods: Fifty patients were treated with a nonablative FXD followed by application of a topical nanofat biocrème. Harvested fat was processed into nanofat, which was compounded with a transdermal liposomal delivery vector to produce a topical biocrème. In two patients, post-auricular skin punch biopsies were performed pre/post-treatment and examined for histological changes. Photos of a historical group treated with only the FXD laser were compared to photos of the patients treated with topical nanofat biocrème and a FXD laser. Results: Findings from postauricular skin biopsies show the skin exposed to FXD with nanofat biocrème had more elastin fibers and a slight increase in the thickness of the epidermis. Patients having FXD plus nanofat biocrème had a statistically significant improvement in the degree of wrinkles, nasolabial fold depth and texture compared to historical controls. Conclusions: Transdermal delivery of nanofat topical biocrème applied after fractional laser treatment can serve as a delivery system to improve fine lines, depth of nasolabial folds and overall texture of the tissue to a greater degree than laser resurfacing alone.