研究目的
Investigating the photodynamic bactericidal effect of Rose Bengal combined with green LED light against Staphylococcus aureus and Salmonella Typhimurium using response surface methodology.
研究成果
The polynomial model developed could provide accurate information on the combined influences of RB and green LED light in aPDT treatment. This combination was able to inactivate S. aureus and S. Typhimurium, showing loss of membrane integrity, morphological changes, and internal cell structural alterations.
研究不足
The study was conducted on a laboratory scale, and the effectiveness of the treatment in real-world food processing environments was not evaluated. The study also did not explore the potential for microbial resistance development to photodynamic therapy.
1：Experimental Design and Method Selection:
A Rotational Central Composite Design was used to establish the conditions for the photoinhibitory activity of RB against S. Typhimurium and S. aureus. The PS concentrations and illumination times were predetermined in preliminary studies.
2：Sample Selection and Data Sources:
Salmonella enterica serotype Typhimurium (ATCC 14028) and Staphylococcus aureus (ATCC 25923) were used.
3：List of Experimental Equipment and Materials:
Brain and Heart Infusion Broth, Tryptic Soy Broth, Rose Bengal, green LED homemade device prototype, spectrofluorimeter, flow cytometer, scanning electron microscope, transmission electron microscope.
4：Experimental Procedures and Operational Workflow:
Bacterial suspension was homogenized with RB at different concentrations and illuminated with green LED light. Controls were also evaluated. After treatment, samples were diluted and plated to determine the number of culturable cells.
5：Data Analysis Methods:
A second-order polynomial model was used for fitting the experimental data and its coefficients were obtained by multiple linear regression.
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