研究目的
To measure alterations of chemical elements’ levels in the aqueous humor of patients undergoing cataract surgery and to understand the causes that may play a role in the initiation and progression of lens opacity.
研究成果
The study identified elevated levels of toxic elements in the aqueous humor of cataract patients, suggesting a potential link between these elements and cataract formation. The findings highlight the importance of further research into the role of trace elements in eye diseases.
研究不足
The study's limitations include the variability in patients' exposure to toxic elements and the need for further research to understand the implications of the findings.
1:Experimental Design and Method Selection:
The study included 115 patients undergoing cataract surgery. The aqueous levels of elements were measured using inductively coupled plasma optical emission spectrometry (ICP-OES).
2:Sample Selection and Data Sources:
Aqueous humor samples were collected from the anterior chamber of the eye of patients.
3:List of Experimental Equipment and Materials:
ICP-OES spectrometer Agilent 5110, microwave mineralization system Mars 6, polypropylene tubes, nitric acid HNO
4:Experimental Procedures and Operational Workflow:
Samples were stored at -80°C, underwent wet mineralization with HNO3, heated to 180°C, diluted, and analyzed by ICP-OES.
5:Data Analysis Methods:
Hierarchical cluster analysis was used to classify elements based on their concentration.
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