1：Experimental Design and Method Selection:

The study involved the synthesis of gold nanorods (GNRs) and CdSe/CdZnS quantum dots (QDs), followed by the formation of electrostatic complexes between them. The distance between GNRs and QDs was controlled by coating GNRs with polyelectrolyte (PE) layers. The fluorescence enhancement was studied under excitation by a 532-nm cw Nd-laser.

2：Sample Selection and Data Sources:

GNRs were synthesized via a seed-mediated growth method, and QDs were synthesized and solubilized in water. The complexes were formed by adding QDs to GNRs solutions to achieve specific QD/GNR ratios.

3：List of Experimental Equipment and Materials:

Transmission electron microscopy (TEM) images were acquired with a Hitachi S-806TEM/SEM electron microscope. Vis-NIR spectra were measured on a Cary 500 Scan UV-vis/near-IR spectrophotometer. Zeta-potential were measured on a Malvern Zetasizer Nano ZS90. Fluorescence was studied with a LN-cooled charge-coupled-device (CCD) coupled to a grating spectrometer.

4：Fluorescence was studied with a LN-cooled charge-coupled-device (CCD) coupled to a grating spectrometer. Experimental Procedures and Operational Workflow:

4. Experimental Procedures and Operational Workflow: The procedure included the synthesis of GNRs and QDs, coating GNRs with PE layers, formation of GNR-QD complexes, and fluorescence measurements.

5：Data Analysis Methods:

The fluorescence enhancement factor was calculated as the product of the incident field intensity enhancement and the change of the quantum efficiency because of the modulation of radiative and nonradiative rates near a nanoparticle.