研究目的
Investigating the super-resolution imaging of label-free transparent dielectric objects with sub-wavelength features using a microsphere-assisted bright-field microscope.
研究成果
The study demonstrates that placing a label-free transparent dielectric object with a periodic structure on a Ag film coated glass slide can generate enhanced period plasmonic near-field illumination due to the excitations of surface plasmon polaritions, improving the imaging resolution of a microsphere-assisted optical microscope.
研究不足
The study is limited to periodic structures of transparent dielectric objects. The resolution improvement is dependent on the excitation of surface plasmon polarition modes, which may not be applicable to all types of samples.
1:Experimental Design and Method Selection:
The study uses a microsphere-assisted bright-field microscope to image label-free transparent dielectric objects. A BaTiO3 glass (BTG) microsphere is coupled with a classical optical microscope for super-resolution imaging.
2:Sample Selection and Data Sources:
Two-dimensional arrays of hexagonally close-packed polystyrene (PS) nanoparticles with diameters of 250, 300, and 400 nm are self-assembled on different substrates (glass slide coated with a Ag film, high reflectance dielectric multilayer, and plain glass slide).
3:List of Experimental Equipment and Materials:
A 30-μm-diameter BTG microsphere (n=1.95), polydimethylsiloxane (PDMS), PS nanoparticles, glass slides coated with Ag film, high reflectance dielectric multilayer, and plain glass slides.
4:95), polydimethylsiloxane (PDMS), PS nanoparticles, glass slides coated with Ag film, high reflectance dielectric multilayer, and plain glass slides. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: PS nanoparticle arrays are self-assembled on different substrates. BTG microspheres are dropped onto the PS nanoparticle array surface, and a layer of PDMS is spin-coated on the microspheres. The virtual image magnified by the BTG microsphere is observed through a 100× objective lens and recorded by a CCD camera.
5:Data Analysis Methods:
The modulation of the images is calculated to quantify the image contrast. Reflectance spectra of the samples are measured and simulated using a FDTD method to understand the contributions of different modes in imaging.
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CCD camera
Leica-DFC295
Leica
Used to record the images observed through the microscope.
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Oceanoptics-USB4000 spectrometer
USB4000
Oceanoptics
Used to measure the reflectances of the substrates.
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BTG microsphere
30-μm-diameter
Acts as a lens to assist in super-resolution imaging by converting evanescent waves into propagating waves.
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PS nanoparticles
250, 300, and 400 nm diameter
Used as the transparent dielectric objects for imaging.
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PDMS
Used to immerse the BTG microspheres for imaging.
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SEM
TEOL JSM-5610LV
TEOL
Used to characterize the surface structure of the PS nanoparticle arrays.
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