研究目的
To develop a selective and sensitive fluorescent probe for the detection and distinction of biothiols, such as glutathione (GSH) and cysteine (Cys), based on the inner filter effect (IFE).
研究成果
The “CDs-AgOH colloid” and “CDs-AgOH colloid-Cys” probe systems based on the IFE mechanism effectively distinguished and quantified Cys and GSH with detection limits of 7.2 μM and 3.6 μM, respectively. The probe was successfully applied for GSH detection in a serum sample, demonstrating its potential for practical applications in biothiol detection.
研究不足
The study focused on the detection of Cys and GSH, and the probe's performance with other biothiols like homocysteine (Hcy) was not explored. The interference study was limited to a set of amino acids and metal ions, and real sample analysis was only conducted with FBS.
1：Experimental Design and Method Selection:
The study utilized carbon quantum dots (CDs) synthesized by a hydrothermal method with citric acid, ethylenediamine, and 1,3-propylenediamine. The fluorescent probe was constructed by combining CDs and AgOH colloids to investigate the fluorescent performance in the presence of different biothiols (Cys, GSH).
2：Sample Selection and Data Sources:
The study used biothiols (Cys and GSH) and other amino acids to test the selectivity and sensitivity of the probe system. Real sample analysis was conducted using a fetal bovine serum (FBS) sample.
3：List of Experimental Equipment and Materials:
Instruments included a FEI TF-20 microscope for HRTEM images, LS-55 fluorescence spectrometer for fluorescence spectra, Magna-IR560 FT-IR spectrometer for FT-IR spectrum, AXIS ULTRA DLD X-ray photoelectron spectrometer for XPS images, and UV-2550 spectrophotometer for UV–vis absorption spectra. Materials included citric acid, ethylenediamine, 1,3-propanediamine, and various amino acids and metal ions.
4：Experimental Procedures and Operational Workflow:
The CDs were synthesized and characterized. The probe systems “CDs-AgOH colloid” and “CDs-AgOH colloid-Cys” were constructed and tested for their response to biothiols. The pH and interference from other amino acids were optimized. The probe was applied to detect GSH in an FBS sample.
5：Data Analysis Methods:
The fluorescence response was analyzed to determine the linear relationship between fluorescence intensity and biothiol concentration, and the detection limit was calculated.
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