研究目的
Investigating the efficiency of different solvents for extracting histamine in tuna fish and developing a rapid and simple method for histamine determination using DESIR coupled with NIR spectroscopy.
研究成果
The DESIR method coupled with NIR spectroscopy offers a rapid and simple alternative for histamine determination in tuna fish, with boiling water as the most cost-effective solvent. However, further improvements are needed to enhance accuracy, possibly through the development of a preconcentration process or the use of more selective solvents.
研究不足
The error from DESIR and NIR techniques was higher than the standard error of the reference method, making it suitable for screening but not for precise quantification. The method may also be affected by interference from other compounds extracted along with histamine.
1：Experimental Design and Method Selection:
The study compared the efficiency of three solvents (75% methanol, 100% methanol, and boiling water) for histamine extraction in tuna fish using the DESIR method coupled with NIR spectroscopy.
2：Sample Selection and Data Sources:
Skipjack tuna samples were used, with histamine content determined by the AOAC fluorometric method as a reference.
3：List of Experimental Equipment and Materials:
Equipment included a Fourier transform NIR spectrometer, microwave oven, rotating knife chopper, homogenizer, water bath, fluorimeter, and glass microfibre filter paper.
4：Experimental Procedures and Operational Workflow:
Samples were prepared by the DESIR method, dried, and then analyzed by NIR spectroscopy. Calibration equations were developed and validated.
5：Data Analysis Methods:
Partial least squares regression was used for data analysis, with performance evaluated based on standard error of prediction, bias, and coefficient of determination.
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