摘要： Laser-Induced Breakdown Spectroscopy (LIBS) is an optical analytical technique with a multi-element capability for element bioimaging in plants. During the years of LIBS development, the major application field has been in industry. However, during the last two decades, LIBS became a useful imaging tool in various biologic matrices, e.g. bones, mammals’ organs, and in the plant science. In this work, we present an overview of LIBS achievements in plant bioimaging which started in 2006. The progress in the assessment of spatial element distribution in plants is documented here with respect to the applications in phytotoxicity testing for the following reason: the information on the spatial distribution of elements can reveal a relationship between the exact location of an element and its toxic effect. This review discusses the state of the art of various elements’ bioimaging in plants using LIBS with a spatial resolution at micrometer scale.