研究目的
To develop a novel optical fiber glucose biosensor based on carbon quantum dots-glucose oxidase/cellulose acetate complex sensitive film for the detection of low concentration glucose with high sensitivity and repeatability.
研究成果
The developed fiber-optic biosensor based on the CQDs-GOD/CA complex sensitive film exhibits high sensitivity, selectivity, and repeatability for the detection of low concentration glucose. It provides a promising platform for trace glucose sensing applications in environmental monitoring, food analysis, and biomedical fields.
研究不足
The exactness, sensitivity, and detection limit of the sensor in the quantitative analysis of trace glucose are restricted due to the small difference in fluorescence signal change. Future work will focus on optimizing the sensor's performance through improvements in the signal collection system and sensing probe preparation.
1：Experimental Design and Method Selection:
The study involved the fabrication of a CQDs-GOD/CA complex sensitive film using the dip-coating method to immobilize the film onto the end face of an optical fiber. The film's properties were characterized using FESEM, AFM, FTIR, and spectrophotometry.
2：Sample Selection and Data Sources:
Standard glucose solutions with various concentrations were prepared for testing the biosensor's performance.
3：List of Experimental Equipment and Materials:
Equipment included a fluorescence spectrophotometer, FT-IR spectrometer, inverted microscope, AFM, FESEM, and a 405 nm violet diode laser for the optical sensing platform.
4：Experimental Procedures and Operational Workflow:
The biosensor's response to glucose was measured by dipping the probe into glucose solutions and recording the fluorescence intensity changes.
5：Data Analysis Methods:
The fluorescence quenching data were analyzed using the modified Stern-Volmer equation to establish the relationship between fluorescence intensity and glucose concentration.
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