摘要： Photonic crystal fiber (PCF) sensor based on surface plasmon resonance (SPR) has broad application prospects in the detection of biological proteins, DNA/RNA, and toxic chemicals. However, there are many problems in application process, such as inhomogeneous metal nanofilm, low filling speed of analyte, and low detection efficiency, which limit the development of this technology. In this paper, a dual-channel PCF sensor based on simultaneous detection which has the advantages of SPR and PCF was proposed to improve the detection efficiency of samples. By optimizing the dielectric layer, the identification ability of channels is effectively improved, and wavelength sensitivities of two channels could reached 11,600 nm/RIU and 10,600 nm/RIU, respectively. Based on this, the horizontal polishing structure was proposed, then the PCF could be immersed in the liquid analyte directly, which can reduce the difficulty of coating and avoid the filling of microfluid. The proposed structure has important scientific significance and application value to promote the practical development of SPR technology with high sensitivity in biochemical sensing and reduce the process complexity in the detection of multi-sample.