研究目的
Investigating the lipid droplet composition and distribution in medaka fish eggs during development using vibrational spectroscopic imaging techniques.
研究成果
The study demonstrated that vibrational spectroscopic imaging techniques (NIR, MIR, and Raman) are effective tools for analyzing the chemical composition and distribution of lipid droplets in medaka fish eggs during development. The findings provide insights into the dynamic biological processes occurring in the eggs, including changes in lipid composition and interactions with other cellular components.
研究不足
The study is limited by the need for sample preparation for Raman and MIR imaging, which may affect the natural state of the eggs. Additionally, the interpretation of NIR spectra can be complex due to overlapping absorption bands.
1：Experimental Design and Method Selection:
The study employed NIR, MIR, and Raman spectroscopic imaging to analyze the chemical composition of lipid droplets in medaka fish eggs at different developmental stages.
2：Sample Selection and Data Sources:
Fertilized medaka fish eggs were collected on the 1st, 5th, 9th, and 13th day after fertilization.
3：List of Experimental Equipment and Materials:
NIR imaging was performed with a spatial resolution of 25 μm. MIR imaging used an Agilent 670-IR spectrometer with a 620-IR microscope and a liquid nitrogen cooled MCT FPA detector. Raman imaging was conducted using a Confocal Raman Imaging system (WITec alpha 300).
4：0). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Eggs were fixed and cross-sectioned for Raman and MIR imaging. NIR measurements were carried out in vitro and in situ.
5：Data Analysis Methods:
Principal Component Analysis (PCA) was used for NIR data, and k-means cluster analysis (KMC) and unsupervised hierarchical cluster analysis (UHCA) were employed for Raman and MIR data, respectively.
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