研究目的
Investigating the antimicrobial activity of a chitosan-based hydrogel embedded with gold nanorods (Ch/AuNRs) using plasmonic photothermal therapy (PPTT) against a panel of pathogenic bacteria and fungi, including clinical multidrug-resistant isolates, and assessing its cytotoxicity, cellular proliferation, and immune response in a murine macrophage model.
研究成果
The Ch/AuNR hydrogel combined with PPTT demonstrated potent antimicrobial activity against a wide range of pathogens, including multidrug-resistant strains, with minimal cytotoxicity and no adverse effects on macrophage proliferation. The hydrogel also elicited a balanced immune response, suggesting its potential as a local treatment for chronic nosocomial infections. Further in vivo studies are recommended to validate these findings.
研究不足
The study's limitations include the in vitro nature of the experiments, which may not fully replicate in vivo conditions. Additionally, the cytotoxicity at higher concentrations of Ch/AuNRs and the need for further in vivo studies to ensure safety and efficacy were noted.
1：Experimental Design and Method Selection:
The study involved the synthesis and characterization of gold nanorods (AuNRs) and their embedding into a chitosan-based hydrogel (Ch/AuNRs). The antimicrobial activity was assessed against Gram-positive and Gram-negative bacteria, including multidrug-resistant strains, and pathogenic fungi. The cytotoxic effect, cellular proliferation, and cytokine expression were evaluated in a murine macrophage model.
2：Sample Selection and Data Sources:
Bacterial and fungal strains included ATCC strains and clinical isolates. The murine macrophage cell line Raw
3：7 was used for cytotoxicity and proliferation assays. List of Experimental Equipment and Materials:
2 Equipment included a UV-VIS spectrophotometer, transmission electron microscope (TEM), environmental scanning electron microscope (ESEM), and energy-dispersive X-ray spectroscopy (EDX). Materials included chitosan, gold nanorods, and an infrared diode laser.
4：Experimental Procedures and Operational Workflow:
AuNRs were synthesized and characterized. Hydrogels were prepared and characterized. Antimicrobial activity was measured via MIC and MBC/MFC assays. Cytotoxicity and proliferation were assessed using MTT and BrdU assays, respectively. Cytokine levels were measured using ELISA.
5：Data Analysis Methods:
Statistical significance was determined using one-way ANOVA and a Bonferroni post hoc test.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
